Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog.
For many years, modifications of the uterine extracellular matrix (ECM) during gestation have not been considered as critical for successful canine (Canis lupus familiaris) pregnancy. However previous reports indicated an effect of free-floating blastocysts on the composition of the uterine ECM. Her...
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ftunivbern:oai:boris.unibe.ch:114640 2023-08-20T04:05:49+02:00 Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P 2018-05 application/pdf https://boris.unibe.ch/114640/1/REP-17-0761.full.pdf https://boris.unibe.ch/114640/ eng eng BioScientifica https://boris.unibe.ch/114640/ info:eu-repo/semantics/openAccess Graubner, Felix R; Boos, Alois; Aslan, Selim; Kücükaslan, Ibrahim; Kowalewski, Mariusz P (2018). Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. Reproduction, 155(5), pp. 403-421. BioScientifica 10.1530/REP-17-0761 <http://dx.doi.org/10.1530/REP-17-0761> 570 Life sciences biology 610 Medicine & health info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion PeerReviewed 2018 ftunivbern https://doi.org/10.1530/REP-17-0761 2023-07-31T21:43:06Z For many years, modifications of the uterine extracellular matrix (ECM) during gestation have not been considered as critical for successful canine (Canis lupus familiaris) pregnancy. However previous reports indicated an effect of free-floating blastocysts on the composition of the uterine ECM. Here, the expression of selected genes involved in structural functions, cell-to-cell communication and inhibition of matrix metalloproteinases were targeted utilizing qPCR and immunohistochemistry. We found that canine free-floating embryos affect gene expression of FN1, ECM1 and TIMP4. This seems to be associated with modulation of trophoblast invasion, and proliferative and adhesive functions of the uterus. Although not modulated at the beginning of pregnancy, the decrease of structural ECM components (i.e., COL1, -3, -4 and LAMA) from pre-implantation towards post-implantation at placentation sites appears to be associated with softening of the tissue in preparation for trophoblast invasion. The further decrease of these components at placentation sites at the time of prepartum luteolysis seems to be associated with preparation for the release of fetal membranes. Reflecting a high degree of communication, intercellular cell adhesion molecules are induced following placentation (Cx26), or increase gradually towards prepartum luteolysis (Cx43). The spatio-temporal expression of TIMPs suggests their active involvement in modulating of fetal invasiveness, and together with ECM1 they appear to protect deeper endometrial structures from trophoblast invasion. With this, the dog appears to be an interesting model for investigating placental functions in other species, e.g., in humans in which Placenta accreta appears to share several similarities with canine subinvolution of placental sites (SIPS). Article in Journal/Newspaper Canis lupus BORIS (Bern Open Repository and Information System, University of Bern) Reproduction 155 5 403 421 |
institution |
Open Polar |
collection |
BORIS (Bern Open Repository and Information System, University of Bern) |
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ftunivbern |
language |
English |
topic |
570 Life sciences biology 610 Medicine & health |
spellingShingle |
570 Life sciences biology 610 Medicine & health Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. |
topic_facet |
570 Life sciences biology 610 Medicine & health |
description |
For many years, modifications of the uterine extracellular matrix (ECM) during gestation have not been considered as critical for successful canine (Canis lupus familiaris) pregnancy. However previous reports indicated an effect of free-floating blastocysts on the composition of the uterine ECM. Here, the expression of selected genes involved in structural functions, cell-to-cell communication and inhibition of matrix metalloproteinases were targeted utilizing qPCR and immunohistochemistry. We found that canine free-floating embryos affect gene expression of FN1, ECM1 and TIMP4. This seems to be associated with modulation of trophoblast invasion, and proliferative and adhesive functions of the uterus. Although not modulated at the beginning of pregnancy, the decrease of structural ECM components (i.e., COL1, -3, -4 and LAMA) from pre-implantation towards post-implantation at placentation sites appears to be associated with softening of the tissue in preparation for trophoblast invasion. The further decrease of these components at placentation sites at the time of prepartum luteolysis seems to be associated with preparation for the release of fetal membranes. Reflecting a high degree of communication, intercellular cell adhesion molecules are induced following placentation (Cx26), or increase gradually towards prepartum luteolysis (Cx43). The spatio-temporal expression of TIMPs suggests their active involvement in modulating of fetal invasiveness, and together with ECM1 they appear to protect deeper endometrial structures from trophoblast invasion. With this, the dog appears to be an interesting model for investigating placental functions in other species, e.g., in humans in which Placenta accreta appears to share several similarities with canine subinvolution of placental sites (SIPS). |
format |
Article in Journal/Newspaper |
author |
Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P |
author_facet |
Graubner, Felix R Boos, Alois Aslan, Selim Kücükaslan, Ibrahim Kowalewski, Mariusz P |
author_sort |
Graubner, Felix R |
title |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. |
title_short |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. |
title_full |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. |
title_fullStr |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. |
title_full_unstemmed |
Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. |
title_sort |
uterine and placental distribution of selected extracellular matrix (ecm) components in the dog. |
publisher |
BioScientifica |
publishDate |
2018 |
url |
https://boris.unibe.ch/114640/1/REP-17-0761.full.pdf https://boris.unibe.ch/114640/ |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Graubner, Felix R; Boos, Alois; Aslan, Selim; Kücükaslan, Ibrahim; Kowalewski, Mariusz P (2018). Uterine and placental distribution of selected extracellular matrix (ECM) components in the dog. Reproduction, 155(5), pp. 403-421. BioScientifica 10.1530/REP-17-0761 <http://dx.doi.org/10.1530/REP-17-0761> |
op_relation |
https://boris.unibe.ch/114640/ |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1530/REP-17-0761 |
container_title |
Reproduction |
container_volume |
155 |
container_issue |
5 |
container_start_page |
403 |
op_container_end_page |
421 |
_version_ |
1774716555189288960 |