Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions
With regard to predicted oceanic warming, we studied the effects of heat stress on the redox system during embryonic development of Atlantic cod (Gadus morhua), with emphasis on the glutathione balance, activities of key antioxidant enzymes, and their mRNA levels. The embryos were incubated at optim...
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Online Access: | http://hdl.handle.net/1956/9755 https://doi.org/10.1016/j.freeradbiomed.2012.11.022 |
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ftunivbergen:oai:bora.uib.no:1956/9755 2023-05-15T15:27:03+02:00 Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions Skjærven, Kaja Helvik Penglase, Samuel Olsvik, Pål Asgeir Hamre, Kristin 2015-04-01T08:27:47Z application/pdf http://hdl.handle.net/1956/9755 https://doi.org/10.1016/j.freeradbiomed.2012.11.022 eng eng Elsevier Norges forskningsråd: 199482 EU: LARVANET – COST action FA0801 urn:issn:0891-5849 http://hdl.handle.net/1956/9755 https://doi.org/10.1016/j.freeradbiomed.2012.11.022 cristin:1030462 Attribution-NonCommercial-NoDerivs CC BY-NC-ND http://creativecommons.org/licenses/by-nc-nd/3.0/ Copyright 2012 Elsevier Inc. Free Radical Biology & Medicine 57 29-38 Atlantic cod Embryo Redox Glutathione Glutathione peroxidase Superoxide dismutase Catalase Free radicals VDP::Agriculture and fisheries science: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og fiskerifag: 900::Fiskerifag: 920::Fiskehelse : 923 Peer reviewed Journal article 2015 ftunivbergen https://doi.org/10.1016/j.freeradbiomed.2012.11.022 2023-03-14T17:39:16Z With regard to predicted oceanic warming, we studied the effects of heat stress on the redox system during embryonic development of Atlantic cod (Gadus morhua), with emphasis on the glutathione balance, activities of key antioxidant enzymes, and their mRNA levels. The embryos were incubated at optimal temperature for development (6 °C) or slightly above the threshold temperature (10 °C). The regulation of all the redox-related parameters measured at optimum development was highly dynamic and complex, indicating the importance of both maternal and zygotic contributions to maintaining redox equilibrium. Development at 10 °C caused a significantly higher mortality at the blastula and early gastrula stages, indicating severe stress. Measures of the glutathione redox couple showed a significantly more reduced state in embryos at 10 °C compared to 6 °C at the post-gastrula stages. Mean normalized expression of nrf2, trxred, g6pd, gclc, nox1, CuZnsod, and mt in embryos kept at 10 °C revealed stage-specific significantly reduced mRNA levels. Activities of antioxidant enzymes changed both during ontogenesis and in response to temperature, but did not correlate with mRNA levels. As the embryos need a tightly regulated redox environment to coordinate between growth and differentiation, these findings suggest that the altered redox balance might participate in inducing phenotypic changes caused by elevated temperature. publishedVersion Article in Journal/Newspaper atlantic cod Gadus morhua University of Bergen: Bergen Open Research Archive (BORA-UiB) Free Radical Biology and Medicine 57 29 38 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Atlantic cod Embryo Redox Glutathione Glutathione peroxidase Superoxide dismutase Catalase Free radicals VDP::Agriculture and fisheries science: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og fiskerifag: 900::Fiskerifag: 920::Fiskehelse : 923 |
spellingShingle |
Atlantic cod Embryo Redox Glutathione Glutathione peroxidase Superoxide dismutase Catalase Free radicals VDP::Agriculture and fisheries science: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og fiskerifag: 900::Fiskerifag: 920::Fiskehelse : 923 Skjærven, Kaja Helvik Penglase, Samuel Olsvik, Pål Asgeir Hamre, Kristin Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions |
topic_facet |
Atlantic cod Embryo Redox Glutathione Glutathione peroxidase Superoxide dismutase Catalase Free radicals VDP::Agriculture and fisheries science: 900::Fisheries science: 920::Fish health: 923 VDP::Landbruks- og fiskerifag: 900::Fiskerifag: 920::Fiskehelse : 923 |
description |
With regard to predicted oceanic warming, we studied the effects of heat stress on the redox system during embryonic development of Atlantic cod (Gadus morhua), with emphasis on the glutathione balance, activities of key antioxidant enzymes, and their mRNA levels. The embryos were incubated at optimal temperature for development (6 °C) or slightly above the threshold temperature (10 °C). The regulation of all the redox-related parameters measured at optimum development was highly dynamic and complex, indicating the importance of both maternal and zygotic contributions to maintaining redox equilibrium. Development at 10 °C caused a significantly higher mortality at the blastula and early gastrula stages, indicating severe stress. Measures of the glutathione redox couple showed a significantly more reduced state in embryos at 10 °C compared to 6 °C at the post-gastrula stages. Mean normalized expression of nrf2, trxred, g6pd, gclc, nox1, CuZnsod, and mt in embryos kept at 10 °C revealed stage-specific significantly reduced mRNA levels. Activities of antioxidant enzymes changed both during ontogenesis and in response to temperature, but did not correlate with mRNA levels. As the embryos need a tightly regulated redox environment to coordinate between growth and differentiation, these findings suggest that the altered redox balance might participate in inducing phenotypic changes caused by elevated temperature. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Skjærven, Kaja Helvik Penglase, Samuel Olsvik, Pål Asgeir Hamre, Kristin |
author_facet |
Skjærven, Kaja Helvik Penglase, Samuel Olsvik, Pål Asgeir Hamre, Kristin |
author_sort |
Skjærven, Kaja Helvik |
title |
Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions |
title_short |
Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions |
title_full |
Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions |
title_fullStr |
Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions |
title_full_unstemmed |
Redox regulation in Atlantic cod (Gadus morhua) embryos developing under normal and heat-stressed conditions |
title_sort |
redox regulation in atlantic cod (gadus morhua) embryos developing under normal and heat-stressed conditions |
publisher |
Elsevier |
publishDate |
2015 |
url |
http://hdl.handle.net/1956/9755 https://doi.org/10.1016/j.freeradbiomed.2012.11.022 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Free Radical Biology & Medicine 57 29-38 |
op_relation |
Norges forskningsråd: 199482 EU: LARVANET – COST action FA0801 urn:issn:0891-5849 http://hdl.handle.net/1956/9755 https://doi.org/10.1016/j.freeradbiomed.2012.11.022 cristin:1030462 |
op_rights |
Attribution-NonCommercial-NoDerivs CC BY-NC-ND http://creativecommons.org/licenses/by-nc-nd/3.0/ Copyright 2012 Elsevier Inc. |
op_doi |
https://doi.org/10.1016/j.freeradbiomed.2012.11.022 |
container_title |
Free Radical Biology and Medicine |
container_volume |
57 |
container_start_page |
29 |
op_container_end_page |
38 |
_version_ |
1766357511229669376 |