Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon

Arginine and methionine are indispensable amino acids (AA) for Atlantic salmon, meaning that salmon is unable to produce these AA endogenously and is fully dependent on dietary supply. In addition to be substrates for protein synthesis, these AA are involved in several metabolic pathways in the fish...

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Published in:British Journal of Nutrition
Main Author: Andersen, Synne Marte
Format: Doctoral or Postdoctoral Thesis
Language:English
Published: The University of Bergen 2014
Subjects:
Online Access:http://hdl.handle.net/1956/8651
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description Arginine and methionine are indispensable amino acids (AA) for Atlantic salmon, meaning that salmon is unable to produce these AA endogenously and is fully dependent on dietary supply. In addition to be substrates for protein synthesis, these AA are involved in several metabolic pathways in the fish. Arginine is used for production of nitric oxide, creatine, urea and polyamines, while methionine is converted to the methyl donor S-adenosyl methionine (SAM), which is important for polyamine production by supplying the aminopropyl donor decarboxylated SAM. Thus, both arginine and methionine may influence polyamine production. Polyamines are essential for cell growth and differentiation and can modulate gene expression and energy metabolism, stabilize proteins and cell membranes. Polyamines can also induce apoptosis and formation of reactive oxygen species under too high concentrations. Dietary arginine supplementation have been demonstrated to reduce visceral mass while increasing lean mass in pigs and rodents, which has been linked to increased energy consumption caused by increased polyamine production. Hence we aimed to investigate whether arginine supplementation to Atlantic salmon could affect growth and deposition pattern, and to what extent any effects was linked to increased production and catabolism of polyamines. We also assessed if methionine affected polyamine production by modulating SAM availability. Two feeding trials were conducted, in juvenile and adult Atlantic salmon, with graded supplementation of arginine beyond the established requirement for growth. Tissue samples were taken to analyse gene expression, amino acids, polyamines and other metabolic parameters. Liver cells were isolated from the adult salmon fed graded inclusions of arginine and cultured in vitro together with activator/inhibitor of polyamine metabolism, in order to assess the importance of polyamine metabolism for cell survival and lipid metabolism. Metabolomic analysis applying HPLC-QTOF MS were performed on liver and plasma ...
format Doctoral or Postdoctoral Thesis
author Andersen, Synne Marte
spellingShingle Andersen, Synne Marte
Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon
author_facet Andersen, Synne Marte
author_sort Andersen, Synne Marte
title Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon
title_short Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon
title_full Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon
title_fullStr Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon
title_full_unstemmed Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon
title_sort interactions of arginine and polyamines on growth and metabolism in atlantic salmon
publisher The University of Bergen
publishDate 2014
url http://hdl.handle.net/1956/8651
genre Atlantic salmon
genre_facet Atlantic salmon
op_relation Paper I: Andersen, S.M., Holen, E., Aksnes, A., Rønnestad, I., Zerrahn, J.E. and Espe, M. (2013). "Dietary arginine affects energy metabolism through polyamine turnover in juvenile Atlantic salmon (Salmo salar)." British Journal of Nutrition 110, 1968-1977. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1017/S0007114513001402 .
Paper II: Andersen, S.M., Holen, E., Aksnes, A., Rønnestad, I., Zerrahn, J.E. and Espe, M. (2014). ”Adult Atlantic salmon (Salmo salar L.) adapts to long-term surplus dietary arginine supplementation.” Aquaculture Nutrition. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1111/anu.12168 .
Paper III: Andersen, S.M., Taylor, R., Holen, E., Aksnes, A. and Espe, M. (2014). “Arginine concentration and exposure time affects polyamine and glucose metabolism in primary liver cells isolated from Atlantic salmon.” Amino Acids 46, 1225-1233. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1007/s00726-014-1684-4 .
Paper IV: Andersen, S.M., Assaad, H.I., Lin, G., Wang, J., Aksnes, A., Wu, G. and Espe, M. (2014). ”Metabolomic analysis of plasma and liver from surplus arginine fed Atlantic salmon.” Frontiers in Bioscience. Full text not available in BORA.
Paper V: Espe, M., Andersen, S.M., Holen, E., Rønnestad, I., Veiseth-Kent, E., Zerrahn, J.E. and Aksnes, A. (2014). ” Methionine deficiency does not increase polyamine turnover through depletion of liver S-adenosylmethionine (SAM) in juvenile Atlantic salmon.” British Journal of Nutrition. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1017/S0007114514002062 .
urn:isbn:978-82-308-2823-6
http://hdl.handle.net/1956/8651
op_rights Copyright the author. All rights reserved
op_doi https://doi.org/10.1017/S000711451300140210.1111/anu.1216810.1007/s00726-014-1684-410.1017/S0007114514002062
container_title British Journal of Nutrition
container_volume 110
container_issue 11
container_start_page 1968
op_container_end_page 1977
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spelling ftunivbergen:oai:bora.uib.no:1956/8651 2023-05-15T15:30:32+02:00 Interactions of arginine and polyamines on growth and metabolism in Atlantic salmon Andersen, Synne Marte 2014-10-09 application/pdf http://hdl.handle.net/1956/8651 eng eng The University of Bergen Paper I: Andersen, S.M., Holen, E., Aksnes, A., Rønnestad, I., Zerrahn, J.E. and Espe, M. (2013). "Dietary arginine affects energy metabolism through polyamine turnover in juvenile Atlantic salmon (Salmo salar)." British Journal of Nutrition 110, 1968-1977. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1017/S0007114513001402 . Paper II: Andersen, S.M., Holen, E., Aksnes, A., Rønnestad, I., Zerrahn, J.E. and Espe, M. (2014). ”Adult Atlantic salmon (Salmo salar L.) adapts to long-term surplus dietary arginine supplementation.” Aquaculture Nutrition. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1111/anu.12168 . Paper III: Andersen, S.M., Taylor, R., Holen, E., Aksnes, A. and Espe, M. (2014). “Arginine concentration and exposure time affects polyamine and glucose metabolism in primary liver cells isolated from Atlantic salmon.” Amino Acids 46, 1225-1233. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1007/s00726-014-1684-4 . Paper IV: Andersen, S.M., Assaad, H.I., Lin, G., Wang, J., Aksnes, A., Wu, G. and Espe, M. (2014). ”Metabolomic analysis of plasma and liver from surplus arginine fed Atlantic salmon.” Frontiers in Bioscience. Full text not available in BORA. Paper V: Espe, M., Andersen, S.M., Holen, E., Rønnestad, I., Veiseth-Kent, E., Zerrahn, J.E. and Aksnes, A. (2014). ” Methionine deficiency does not increase polyamine turnover through depletion of liver S-adenosylmethionine (SAM) in juvenile Atlantic salmon.” British Journal of Nutrition. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1017/S0007114514002062 . urn:isbn:978-82-308-2823-6 http://hdl.handle.net/1956/8651 Copyright the author. All rights reserved Doctoral thesis 2014 ftunivbergen https://doi.org/10.1017/S000711451300140210.1111/anu.1216810.1007/s00726-014-1684-410.1017/S0007114514002062 2023-03-14T17:39:24Z Arginine and methionine are indispensable amino acids (AA) for Atlantic salmon, meaning that salmon is unable to produce these AA endogenously and is fully dependent on dietary supply. In addition to be substrates for protein synthesis, these AA are involved in several metabolic pathways in the fish. Arginine is used for production of nitric oxide, creatine, urea and polyamines, while methionine is converted to the methyl donor S-adenosyl methionine (SAM), which is important for polyamine production by supplying the aminopropyl donor decarboxylated SAM. Thus, both arginine and methionine may influence polyamine production. Polyamines are essential for cell growth and differentiation and can modulate gene expression and energy metabolism, stabilize proteins and cell membranes. Polyamines can also induce apoptosis and formation of reactive oxygen species under too high concentrations. Dietary arginine supplementation have been demonstrated to reduce visceral mass while increasing lean mass in pigs and rodents, which has been linked to increased energy consumption caused by increased polyamine production. Hence we aimed to investigate whether arginine supplementation to Atlantic salmon could affect growth and deposition pattern, and to what extent any effects was linked to increased production and catabolism of polyamines. We also assessed if methionine affected polyamine production by modulating SAM availability. Two feeding trials were conducted, in juvenile and adult Atlantic salmon, with graded supplementation of arginine beyond the established requirement for growth. Tissue samples were taken to analyse gene expression, amino acids, polyamines and other metabolic parameters. Liver cells were isolated from the adult salmon fed graded inclusions of arginine and cultured in vitro together with activator/inhibitor of polyamine metabolism, in order to assess the importance of polyamine metabolism for cell survival and lipid metabolism. Metabolomic analysis applying HPLC-QTOF MS were performed on liver and plasma ... Doctoral or Postdoctoral Thesis Atlantic salmon University of Bergen: Bergen Open Research Archive (BORA-UiB) British Journal of Nutrition 110 11 1968 1977