Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism
Background: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the most abundant among PCBs detected in animal tissues, its mechanism of toxicity is not well understood. Only few studies have been conducted...
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Online Access: | https://hdl.handle.net/1956/8467 https://doi.org/10.1186/1471-2164-15-481 |
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ftunivbergen:oai:bora.uib.no:1956/8467 2023-05-15T15:27:16+02:00 Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism Yadetie, Fekadu Karlsen, Odd André Eide, Marta Hogstrand, Christer Goksøyr, Anders 2014-08-27T11:32:29Z text/xml application/pdf application/vnd.ms-excel application/msword application/octet-stream https://hdl.handle.net/1956/8467 https://doi.org/10.1186/1471-2164-15-481 eng eng BioMed Central urn:issn:1471-2164 https://hdl.handle.net/1956/8467 https://doi.org/10.1186/1471-2164-15-481 cristin:1156946 Attribution CC BY http://creativecommons.org/licenses/by/4.0 Fekadu Yadetie et al.; licensee BioMed Central Ltd. Copyright 2014 Yadetie et al.; licensee BioMed Central Ltd. 481 BMC Genomics 15 Peer reviewed Journal article 2014 ftunivbergen https://doi.org/10.1186/1471-2164-15-481 2023-03-14T17:39:52Z Background: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the most abundant among PCBs detected in animal tissues, its mechanism of toxicity is not well understood. Only few studies have been conducted to explore genes and pathways affected by PCB 153 by using high throughput transcriptomics approaches. To obtain better insights into toxicity mechanisms, we treated juvenile Atlantic cod (Gadus morhua) with PCB 153 (0.5, 2 and 8 mg/kg body weight) for 2 weeks and performed gene expression analysis in the liver using oligonucleotide arrays. Results: Whole-genome gene expression analysis detected about 160 differentially regulated genes. Functional enrichment, interactome, network and gene set enrichment analysis of the differentially regulated genes suggested that pathways associated with cell cycle, lipid metabolism, immune response, apoptosis and stress response were among the top significantly enriched. Particularly, genes coding for proteins in DNA replication/cell cycle pathways and enzymes of lipid biosynthesis were up-regulated suggesting increased cell proliferation and lipogenesis, respectively. Conclusions: PCB 153 appears to activate cell proliferation and lipogenic genes in cod liver. Transcriptional up-regulation of marker genes for lipid biosynthesis resembles lipogenic effects previously reported for persistent organic pollutants (POPs) and other environmental chemicals. Our results provide new insights into mechanisms of PCB 153 induced toxicity. Peer Reviewed publishedVersion Article in Journal/Newspaper atlantic cod Gadus morhua University of Bergen: Bergen Open Research Archive (BORA-UiB) BMC Genomics 15 1 481 |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
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English |
description |
Background: Polychlorinated biphenyls (PCBs) are persistent organic pollutants (POPs) with harmful effects in animals and humans. Although PCB 153 is one of the most abundant among PCBs detected in animal tissues, its mechanism of toxicity is not well understood. Only few studies have been conducted to explore genes and pathways affected by PCB 153 by using high throughput transcriptomics approaches. To obtain better insights into toxicity mechanisms, we treated juvenile Atlantic cod (Gadus morhua) with PCB 153 (0.5, 2 and 8 mg/kg body weight) for 2 weeks and performed gene expression analysis in the liver using oligonucleotide arrays. Results: Whole-genome gene expression analysis detected about 160 differentially regulated genes. Functional enrichment, interactome, network and gene set enrichment analysis of the differentially regulated genes suggested that pathways associated with cell cycle, lipid metabolism, immune response, apoptosis and stress response were among the top significantly enriched. Particularly, genes coding for proteins in DNA replication/cell cycle pathways and enzymes of lipid biosynthesis were up-regulated suggesting increased cell proliferation and lipogenesis, respectively. Conclusions: PCB 153 appears to activate cell proliferation and lipogenic genes in cod liver. Transcriptional up-regulation of marker genes for lipid biosynthesis resembles lipogenic effects previously reported for persistent organic pollutants (POPs) and other environmental chemicals. Our results provide new insights into mechanisms of PCB 153 induced toxicity. Peer Reviewed publishedVersion |
format |
Article in Journal/Newspaper |
author |
Yadetie, Fekadu Karlsen, Odd André Eide, Marta Hogstrand, Christer Goksøyr, Anders |
spellingShingle |
Yadetie, Fekadu Karlsen, Odd André Eide, Marta Hogstrand, Christer Goksøyr, Anders Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
author_facet |
Yadetie, Fekadu Karlsen, Odd André Eide, Marta Hogstrand, Christer Goksøyr, Anders |
author_sort |
Yadetie, Fekadu |
title |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_short |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_full |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_fullStr |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_full_unstemmed |
Liver transcriptome analysis of Atlantic cod (Gadus morhua) exposed to PCB 153 indicates effects on cell cycle regulation and lipid metabolism |
title_sort |
liver transcriptome analysis of atlantic cod (gadus morhua) exposed to pcb 153 indicates effects on cell cycle regulation and lipid metabolism |
publisher |
BioMed Central |
publishDate |
2014 |
url |
https://hdl.handle.net/1956/8467 https://doi.org/10.1186/1471-2164-15-481 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
481 BMC Genomics 15 |
op_relation |
urn:issn:1471-2164 https://hdl.handle.net/1956/8467 https://doi.org/10.1186/1471-2164-15-481 cristin:1156946 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0 Fekadu Yadetie et al.; licensee BioMed Central Ltd. Copyright 2014 Yadetie et al.; licensee BioMed Central Ltd. |
op_doi |
https://doi.org/10.1186/1471-2164-15-481 |
container_title |
BMC Genomics |
container_volume |
15 |
container_issue |
1 |
container_start_page |
481 |
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1766357712091742208 |