Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.)
Background: Under-dimensioned hearts causing functional problems are associated with higher mortality rates in intensive Atlantic salmon aquaculture. Previous studies have indicated that tetradecylthioacetic acid (TTA) induces cardiac growth and also stimulates transcription of peroxisome proliferat...
Published in: | BMC Genomics |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BioMed Central
2013
|
Subjects: | |
Online Access: | https://hdl.handle.net/1956/7898 https://doi.org/10.1186/1471-2164-13-180 |
id |
ftunivbergen:oai:bora.uib.no:1956/7898 |
---|---|
record_format |
openpolar |
spelling |
ftunivbergen:oai:bora.uib.no:1956/7898 2023-05-15T15:29:29+02:00 Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.) Grammes, Fabian Rørvik, Kjell-Arne Thomassen, Magny S. Berge, Rolf Kristian Takle, Harald Rune 2013-08-23T09:19:43Z application/pdf text/csv text/xml https://hdl.handle.net/1956/7898 https://doi.org/10.1186/1471-2164-13-180 eng eng BioMed Central urn:issn:1471-2164 https://hdl.handle.net/1956/7898 https://doi.org/10.1186/1471-2164-13-180 cristin:974535 Attribution CC BY http://creativecommons.org/licenses/by/2.0 Copyright 2012 Grammes et al.; licensee BioMed Central Ltd. Fabian Grammes et al.; licensee BioMed Central Ltd. 180 BMC Genomics 13 Peer reviewed Journal article 2013 ftunivbergen https://doi.org/10.1186/1471-2164-13-180 2023-03-14T17:42:28Z Background: Under-dimensioned hearts causing functional problems are associated with higher mortality rates in intensive Atlantic salmon aquaculture. Previous studies have indicated that tetradecylthioacetic acid (TTA) induces cardiac growth and also stimulates transcription of peroxisome proliferator activated receptors (PPAR) α and β in the Atlantic salmon heart. Since cardiac and transcriptional responses to feed are of high interest in aquaculture, the objective of this study was to characterize the transcriptional mechanisms induced by TTA in the heart of Atlantic salmon. Results: Atlantic salmon were kept at sea for 17 weeks. During the first 8 weeks the fish received a TTA supplemented diet. Using microarrays, profound transcriptional effects were observed in the heart at the end of the experiment, 9 weeks after the feeding of TTA stopped. Approximately 90% of the significant genes were expressed higher in the TTA group. Hypergeometric testing revealed the over-representation of 35 gene ontology terms in the TTA fed group. The GO terms were generally categorized into cardiac performance, lipid catabolism, glycolysis and TCA cycle. Conclusions: Our results indicate that TTA has profound effects on cardiac performance based on results from microarray and qRT-PCR analysis. The gene expression profile favors a scenario of ”physiological”lright hypertrophy recognized by increased oxidative fatty acid metabolism, glycolysis and TCA cycle activity as well as cardiac growth and contractility in the heart ventricle. Increased cardiac efficiency may offer significant benefits in the demanding Aquaculture situations. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) BMC Genomics 13 1 180 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
Background: Under-dimensioned hearts causing functional problems are associated with higher mortality rates in intensive Atlantic salmon aquaculture. Previous studies have indicated that tetradecylthioacetic acid (TTA) induces cardiac growth and also stimulates transcription of peroxisome proliferator activated receptors (PPAR) α and β in the Atlantic salmon heart. Since cardiac and transcriptional responses to feed are of high interest in aquaculture, the objective of this study was to characterize the transcriptional mechanisms induced by TTA in the heart of Atlantic salmon. Results: Atlantic salmon were kept at sea for 17 weeks. During the first 8 weeks the fish received a TTA supplemented diet. Using microarrays, profound transcriptional effects were observed in the heart at the end of the experiment, 9 weeks after the feeding of TTA stopped. Approximately 90% of the significant genes were expressed higher in the TTA group. Hypergeometric testing revealed the over-representation of 35 gene ontology terms in the TTA fed group. The GO terms were generally categorized into cardiac performance, lipid catabolism, glycolysis and TCA cycle. Conclusions: Our results indicate that TTA has profound effects on cardiac performance based on results from microarray and qRT-PCR analysis. The gene expression profile favors a scenario of ”physiological”lright hypertrophy recognized by increased oxidative fatty acid metabolism, glycolysis and TCA cycle activity as well as cardiac growth and contractility in the heart ventricle. Increased cardiac efficiency may offer significant benefits in the demanding Aquaculture situations. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Grammes, Fabian Rørvik, Kjell-Arne Thomassen, Magny S. Berge, Rolf Kristian Takle, Harald Rune |
spellingShingle |
Grammes, Fabian Rørvik, Kjell-Arne Thomassen, Magny S. Berge, Rolf Kristian Takle, Harald Rune Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.) |
author_facet |
Grammes, Fabian Rørvik, Kjell-Arne Thomassen, Magny S. Berge, Rolf Kristian Takle, Harald Rune |
author_sort |
Grammes, Fabian |
title |
Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.) |
title_short |
Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.) |
title_full |
Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.) |
title_fullStr |
Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.) |
title_full_unstemmed |
Genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of Atlantic Salmon (Salmo salar L.) |
title_sort |
genome wide response to dietary tetradecylthioacetic acid supplementation in the heart of atlantic salmon (salmo salar l.) |
publisher |
BioMed Central |
publishDate |
2013 |
url |
https://hdl.handle.net/1956/7898 https://doi.org/10.1186/1471-2164-13-180 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
180 BMC Genomics 13 |
op_relation |
urn:issn:1471-2164 https://hdl.handle.net/1956/7898 https://doi.org/10.1186/1471-2164-13-180 cristin:974535 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/2.0 Copyright 2012 Grammes et al.; licensee BioMed Central Ltd. Fabian Grammes et al.; licensee BioMed Central Ltd. |
op_doi |
https://doi.org/10.1186/1471-2164-13-180 |
container_title |
BMC Genomics |
container_volume |
13 |
container_issue |
1 |
container_start_page |
180 |
_version_ |
1766359899660353536 |