Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin
Background: Ten generations of domestication selection has caused farmed Atlantic salmon Salmo salar L. to deviate from wild salmon in a range of traits. Each year hundreds of thousands of farmed salmon escape into the wild. Thus, interbreeding between farmed escapees and wild conspecifics represent...
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ftunivbergen:oai:bora.uib.no:1956/6675 2023-05-15T15:31:29+02:00 Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin Solberg, Monica F. Kvamme, Bjørn Olav Nilsen, Frank Glover, Kevin 2012-12-05 application/pdf text/xml application/vnd.ms-excel http://hdl.handle.net/1956/6675 https://doi.org/10.1186/1756-0500-5-672 eng eng BioMed Central urn:issn:1756-0500 http://hdl.handle.net/1956/6675 https://doi.org/10.1186/1756-0500-5-672 cristin:1003266 Attribution CC BY http://creativecommons.org/licenses/by/2.0/ Copyright 2012 Solberg et al.; licensee BioMed Central Ltd. BMC Research Notes 5 Atlantic salmon Farmed escapees Introgression Common-garden mRNA leve Insulin-like growth factor Oxidative stress Non additive inheritance Peer reviewed Journal article 2012 ftunivbergen https://doi.org/10.1186/1756-0500-5-672 2023-03-14T17:40:05Z Background: Ten generations of domestication selection has caused farmed Atlantic salmon Salmo salar L. to deviate from wild salmon in a range of traits. Each year hundreds of thousands of farmed salmon escape into the wild. Thus, interbreeding between farmed escapees and wild conspecifics represents a significant threat to the genetic integrity of wild salmon populations. In a previous study we demonstrated how domestication has inadvertently selected for reduced responsiveness to stress in farmed salmon. To complement that study, we have evaluated the expression of seven stress-related genes in head kidney of salmon of farmed, hybrid and wild origin exposed to environmentally induced stress. Results: In general, the crowding stressor used to induce environmental stress did not have a strong impact on mRNA expression levels of the seven genes, except for insulin-like growth factor-1 (IGF-1) that was downregulated in the stress treatment relative to the control treatment. mRNA expression levels of glutathione reductase (GR), Cu/Zn superoxide dismutase (Cu/Zn SOD), Mn superoxide dismutase (Mn SOD), glutathione peroxidase (GP) and IGF-1 were affected by genetic origin, thus expressed significantly different between the salmon of farmed, hybrid or wild origin. A positive relationship was detected between body size of wild salmon and mRNA expression level of the IGF-1 gene, in both environments. No such relationship was observed for the hybrid or farmed salmon. Conclusion: Farmed salmon in this study displayed significantly elevated mRNA levels of the IGF-1 gene relative to the wild salmon, in both treatments, while hybrids displayed a non additive pattern of inheritance. As IGF-1 mRNA levels are positively correlated to growth rate, the observed positive relationship between body size and IGF-1 mRNA levels detected in the wild but neither in the farmed nor the hybrid salmon, could indicate that growth selection has increased IGF-1 levels in farmed salmon to the extent that they may not be limiting growth rate. ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) BMC Research Notes 5 1 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Atlantic salmon Farmed escapees Introgression Common-garden mRNA leve Insulin-like growth factor Oxidative stress Non additive inheritance |
spellingShingle |
Atlantic salmon Farmed escapees Introgression Common-garden mRNA leve Insulin-like growth factor Oxidative stress Non additive inheritance Solberg, Monica F. Kvamme, Bjørn Olav Nilsen, Frank Glover, Kevin Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin |
topic_facet |
Atlantic salmon Farmed escapees Introgression Common-garden mRNA leve Insulin-like growth factor Oxidative stress Non additive inheritance |
description |
Background: Ten generations of domestication selection has caused farmed Atlantic salmon Salmo salar L. to deviate from wild salmon in a range of traits. Each year hundreds of thousands of farmed salmon escape into the wild. Thus, interbreeding between farmed escapees and wild conspecifics represents a significant threat to the genetic integrity of wild salmon populations. In a previous study we demonstrated how domestication has inadvertently selected for reduced responsiveness to stress in farmed salmon. To complement that study, we have evaluated the expression of seven stress-related genes in head kidney of salmon of farmed, hybrid and wild origin exposed to environmentally induced stress. Results: In general, the crowding stressor used to induce environmental stress did not have a strong impact on mRNA expression levels of the seven genes, except for insulin-like growth factor-1 (IGF-1) that was downregulated in the stress treatment relative to the control treatment. mRNA expression levels of glutathione reductase (GR), Cu/Zn superoxide dismutase (Cu/Zn SOD), Mn superoxide dismutase (Mn SOD), glutathione peroxidase (GP) and IGF-1 were affected by genetic origin, thus expressed significantly different between the salmon of farmed, hybrid or wild origin. A positive relationship was detected between body size of wild salmon and mRNA expression level of the IGF-1 gene, in both environments. No such relationship was observed for the hybrid or farmed salmon. Conclusion: Farmed salmon in this study displayed significantly elevated mRNA levels of the IGF-1 gene relative to the wild salmon, in both treatments, while hybrids displayed a non additive pattern of inheritance. As IGF-1 mRNA levels are positively correlated to growth rate, the observed positive relationship between body size and IGF-1 mRNA levels detected in the wild but neither in the farmed nor the hybrid salmon, could indicate that growth selection has increased IGF-1 levels in farmed salmon to the extent that they may not be limiting growth rate. ... |
format |
Article in Journal/Newspaper |
author |
Solberg, Monica F. Kvamme, Bjørn Olav Nilsen, Frank Glover, Kevin |
author_facet |
Solberg, Monica F. Kvamme, Bjørn Olav Nilsen, Frank Glover, Kevin |
author_sort |
Solberg, Monica F. |
title |
Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin |
title_short |
Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin |
title_full |
Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin |
title_fullStr |
Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin |
title_full_unstemmed |
Effects of environmental stress on mRNA expression levels of seven genes related to oxidative stress and growth in Atlantic salmon Salmo salar L. of farmed, hybrid and wild origin |
title_sort |
effects of environmental stress on mrna expression levels of seven genes related to oxidative stress and growth in atlantic salmon salmo salar l. of farmed, hybrid and wild origin |
publisher |
BioMed Central |
publishDate |
2012 |
url |
http://hdl.handle.net/1956/6675 https://doi.org/10.1186/1756-0500-5-672 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
BMC Research Notes 5 |
op_relation |
urn:issn:1756-0500 http://hdl.handle.net/1956/6675 https://doi.org/10.1186/1756-0500-5-672 cristin:1003266 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/2.0/ Copyright 2012 Solberg et al.; licensee BioMed Central Ltd. |
op_doi |
https://doi.org/10.1186/1756-0500-5-672 |
container_title |
BMC Research Notes |
container_volume |
5 |
container_issue |
1 |
_version_ |
1766361999013314560 |