Dietary linoleic acid induces obesity through excessive endocannabinoid activity
Background: Dietary intakes of the n-6 fatty acid linoleic acid (LA, 18:2n-6) have increased dramatically during the 20th century. Replacing fish oil (FO) with vegetable oil (VO) in feed for farmed fish introduces LA and alters the fatty acid composition in Atlantic salmon (Salmo salar L.). LA is th...
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Language: | English |
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The University of Bergen
2012
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ftunivbergen:oai:bora.uib.no:1956/5962 2023-05-15T15:32:17+02:00 Dietary linoleic acid induces obesity through excessive endocannabinoid activity Alvheim, Anita Røyneberg 2012-03-23 application/pdf https://hdl.handle.net/1956/5962 eng eng The University of Bergen Paper I: Anita R. Alvheim, Marian K. Malde, Douglas Osei-Hyiaman, Yuhong Lin, Robert Pawlosky, Lise Madsen, Karsten Kristiansen, Livar Frøyland, Joseph R. Hibbeln. Dietary linoleic acid elevates endogenous 2- AG and anandamide and induces obesity. Obesity e-print ahead of paper, 2012. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1038/oby.2012.38 Paper II: Anita R. Alvheim, Bente E. Torstensen, Yu Hong Lin, Haldis H. Lillefosse, Erik-Jan Lock, Lise Madsen, Livar Frøyland, Joseph R. Hibbeln, Marian K. Malde. Dietary linoleic acid elevates the endocannabinoids 2-AG and AEA, and induces weight gain and inflammation in mice fed a low fat diet. Full text not available in BORA. Paper III: Anita R. Alvheim, Bente E. Torstensen, Yu Hong Lin, Haldis H. Lillefosse, Erik-Jan Lock, Lise Madsen, Joseph R. Hibbeln and Marian K. Malde. Dietary linoleic acid elevates endogenous 2-AG and andandamide in Atlantic salmon (Salmo salar L.) and mice, and induces weight gain and inflammation in mice. Full text not available in BORA. urn:isbn:978-82-308-1999-9 https://hdl.handle.net/1956/5962 Copyright the author. All rights reserved VDP::Medical disciplines: 700::Health sciences: 800::Nutrition: 811 Doctoral thesis 2012 ftunivbergen https://doi.org/10.1038/oby.2012.38 2023-03-14T17:40:33Z Background: Dietary intakes of the n-6 fatty acid linoleic acid (LA, 18:2n-6) have increased dramatically during the 20th century. Replacing fish oil (FO) with vegetable oil (VO) in feed for farmed fish introduces LA and alters the fatty acid composition in Atlantic salmon (Salmo salar L.). LA is the precursor of arachidonic acid (AA, 20:4n-6) the backbone of the endocannabinoids 2-arachidonoylglycerol (2-AG) and anandamide (AEA). A sustained hyperactivity of the endocannabinoid system is believed to play a causal role in the development of obesity and associated metabolic disorders. Here we posit that excessive dietary intake of LA, the precursor of AA, would induce endocannabinoid hyperactivity and promote obesity. Design: LA was isolated as an independent variable to reflect the dietary increase in LA from 1 percent of energy (en%) to 8 en% occurring in the US during the 20th century. Male C57BL/6j mice were exposed to 1 en% LA and 8 en% LA in diets of 35 en% and 60 en% fat from last week of gestation and 14 weeks from weaning (Paper I), and in diets of 12.5 en% and 35 en% fat for 16 weeks from 6 weeks of age (Paper II). To reduce tissue n-6 highly unsaturated fatty acids (HUFA), 1 en% eicosapentaenoic acid (EPA, 20:5n-3)/docosahexaenoic acid (DHA, 22:6n-3) were supplemented to the 8 en% LA diets in Paper I. Atlantic salmon, 340 g, was fed fish oil and soybean oil (SO) for 6 months. Male C57BL76j mice, 6 weeks of age, were fed diets of 35 en% fat based on FO salmon fillet (1 en% LA) and SO salmon fillet (8 en% LA) for 16 weeks (Paper III). Results: Increasing dietary LA from 1 en% to 8 en% elevated AA in phospholipids (AA -PL) with a subsequent elevation in liver 2-AG and anandamide associated with higher food intake, feed efficiency, weight gain and adiposity and increased hypertrophy and inflammation of adipose tissue. Selectively reducing LA to 1 en% reversed the obesogenic properties of a high fat diet. Reducing AA -PL by EPA/DHA supplementation resulted in metabolic patterns resembling 1 en% LA diets. ... Doctoral or Postdoctoral Thesis Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) Obesity 20 10 1984 1994 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
VDP::Medical disciplines: 700::Health sciences: 800::Nutrition: 811 |
spellingShingle |
VDP::Medical disciplines: 700::Health sciences: 800::Nutrition: 811 Alvheim, Anita Røyneberg Dietary linoleic acid induces obesity through excessive endocannabinoid activity |
topic_facet |
VDP::Medical disciplines: 700::Health sciences: 800::Nutrition: 811 |
description |
Background: Dietary intakes of the n-6 fatty acid linoleic acid (LA, 18:2n-6) have increased dramatically during the 20th century. Replacing fish oil (FO) with vegetable oil (VO) in feed for farmed fish introduces LA and alters the fatty acid composition in Atlantic salmon (Salmo salar L.). LA is the precursor of arachidonic acid (AA, 20:4n-6) the backbone of the endocannabinoids 2-arachidonoylglycerol (2-AG) and anandamide (AEA). A sustained hyperactivity of the endocannabinoid system is believed to play a causal role in the development of obesity and associated metabolic disorders. Here we posit that excessive dietary intake of LA, the precursor of AA, would induce endocannabinoid hyperactivity and promote obesity. Design: LA was isolated as an independent variable to reflect the dietary increase in LA from 1 percent of energy (en%) to 8 en% occurring in the US during the 20th century. Male C57BL/6j mice were exposed to 1 en% LA and 8 en% LA in diets of 35 en% and 60 en% fat from last week of gestation and 14 weeks from weaning (Paper I), and in diets of 12.5 en% and 35 en% fat for 16 weeks from 6 weeks of age (Paper II). To reduce tissue n-6 highly unsaturated fatty acids (HUFA), 1 en% eicosapentaenoic acid (EPA, 20:5n-3)/docosahexaenoic acid (DHA, 22:6n-3) were supplemented to the 8 en% LA diets in Paper I. Atlantic salmon, 340 g, was fed fish oil and soybean oil (SO) for 6 months. Male C57BL76j mice, 6 weeks of age, were fed diets of 35 en% fat based on FO salmon fillet (1 en% LA) and SO salmon fillet (8 en% LA) for 16 weeks (Paper III). Results: Increasing dietary LA from 1 en% to 8 en% elevated AA in phospholipids (AA -PL) with a subsequent elevation in liver 2-AG and anandamide associated with higher food intake, feed efficiency, weight gain and adiposity and increased hypertrophy and inflammation of adipose tissue. Selectively reducing LA to 1 en% reversed the obesogenic properties of a high fat diet. Reducing AA -PL by EPA/DHA supplementation resulted in metabolic patterns resembling 1 en% LA diets. ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Alvheim, Anita Røyneberg |
author_facet |
Alvheim, Anita Røyneberg |
author_sort |
Alvheim, Anita Røyneberg |
title |
Dietary linoleic acid induces obesity through excessive endocannabinoid activity |
title_short |
Dietary linoleic acid induces obesity through excessive endocannabinoid activity |
title_full |
Dietary linoleic acid induces obesity through excessive endocannabinoid activity |
title_fullStr |
Dietary linoleic acid induces obesity through excessive endocannabinoid activity |
title_full_unstemmed |
Dietary linoleic acid induces obesity through excessive endocannabinoid activity |
title_sort |
dietary linoleic acid induces obesity through excessive endocannabinoid activity |
publisher |
The University of Bergen |
publishDate |
2012 |
url |
https://hdl.handle.net/1956/5962 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Paper I: Anita R. Alvheim, Marian K. Malde, Douglas Osei-Hyiaman, Yuhong Lin, Robert Pawlosky, Lise Madsen, Karsten Kristiansen, Livar Frøyland, Joseph R. Hibbeln. Dietary linoleic acid elevates endogenous 2- AG and anandamide and induces obesity. Obesity e-print ahead of paper, 2012. Full text not available in BORA due to publisher restrictions. The article is available at: http://dx.doi.org/10.1038/oby.2012.38 Paper II: Anita R. Alvheim, Bente E. Torstensen, Yu Hong Lin, Haldis H. Lillefosse, Erik-Jan Lock, Lise Madsen, Livar Frøyland, Joseph R. Hibbeln, Marian K. Malde. Dietary linoleic acid elevates the endocannabinoids 2-AG and AEA, and induces weight gain and inflammation in mice fed a low fat diet. Full text not available in BORA. Paper III: Anita R. Alvheim, Bente E. Torstensen, Yu Hong Lin, Haldis H. Lillefosse, Erik-Jan Lock, Lise Madsen, Joseph R. Hibbeln and Marian K. Malde. Dietary linoleic acid elevates endogenous 2-AG and andandamide in Atlantic salmon (Salmo salar L.) and mice, and induces weight gain and inflammation in mice. Full text not available in BORA. urn:isbn:978-82-308-1999-9 https://hdl.handle.net/1956/5962 |
op_rights |
Copyright the author. All rights reserved |
op_doi |
https://doi.org/10.1038/oby.2012.38 |
container_title |
Obesity |
container_volume |
20 |
container_issue |
10 |
container_start_page |
1984 |
op_container_end_page |
1994 |
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1766362788776640512 |