Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels
In the present experiment, different levels of pressure were investigated to see if it was possible to induce triploidy at a lower pressure level than previously used for Atlantic cod (Gadus morhua). Newly fertilized eggs (offspring of one male and one female), were exposed to different levels of pr...
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The University of Bergen
2011
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ftunivbergen:oai:bora.uib.no:1956/5572 2023-05-15T15:26:56+02:00 Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels Håvardstun, Susanne 2011-10-31 2022545 bytes application/pdf http://hdl.handle.net/1956/5572 eng eng The University of Bergen http://hdl.handle.net/1956/5572 Copyright the author. All rights reserved Triploid Atlantic cod 751599 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Master thesis 2011 ftunivbergen 2023-03-14T17:42:50Z In the present experiment, different levels of pressure were investigated to see if it was possible to induce triploidy at a lower pressure level than previously used for Atlantic cod (Gadus morhua). Newly fertilized eggs (offspring of one male and one female), were exposed to different levels of pressure and accordingly divided into four experimental groups: 400, 500 and 600 bar, and one control group. Each pressure group received the desired pressure for 5 min, beginning at 180 ºC min post-fertilization. Induction of triploidy occurred at each pressure level used in this experiment. Blood cell diameter analysis showed that pressure treatment had an effect on blood cell diameter; resulting in increased mean blood cell diameter that was correlated with increasing pressure level used. Furthermore, microsatellite loci analysis revealed over 90 % triploid outcome in each experimental group, whereas the remaining part could not be identified as triploids. Overall this study demonstrated successfully triploid induction at both low (400 bar) and medium (500 bar) pressure. Further studies are recommended in order to reveal the optimal pressure level for triploid induction in Atlantic cod in accordance with less severe deformities, reduced effect of sexual maturation, high growth rate and a high flesh quality. This, in combination with all-female production, will potentially be a promising approach for triploidy in large-scale aquaculture production. Master i Havbruksbiologi - Generell havbruksbiologi MAMN-HAV HAV399 Master Thesis atlantic cod Gadus morhua University of Bergen: Bergen Open Research Archive (BORA-UiB) |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Triploid Atlantic cod 751599 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 |
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Triploid Atlantic cod 751599 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 Håvardstun, Susanne Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels |
topic_facet |
Triploid Atlantic cod 751599 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920 VDP::Agriculture and fishery disciplines: 900::Fisheries science: 920::Aquaculture: 922 |
description |
In the present experiment, different levels of pressure were investigated to see if it was possible to induce triploidy at a lower pressure level than previously used for Atlantic cod (Gadus morhua). Newly fertilized eggs (offspring of one male and one female), were exposed to different levels of pressure and accordingly divided into four experimental groups: 400, 500 and 600 bar, and one control group. Each pressure group received the desired pressure for 5 min, beginning at 180 ºC min post-fertilization. Induction of triploidy occurred at each pressure level used in this experiment. Blood cell diameter analysis showed that pressure treatment had an effect on blood cell diameter; resulting in increased mean blood cell diameter that was correlated with increasing pressure level used. Furthermore, microsatellite loci analysis revealed over 90 % triploid outcome in each experimental group, whereas the remaining part could not be identified as triploids. Overall this study demonstrated successfully triploid induction at both low (400 bar) and medium (500 bar) pressure. Further studies are recommended in order to reveal the optimal pressure level for triploid induction in Atlantic cod in accordance with less severe deformities, reduced effect of sexual maturation, high growth rate and a high flesh quality. This, in combination with all-female production, will potentially be a promising approach for triploidy in large-scale aquaculture production. Master i Havbruksbiologi - Generell havbruksbiologi MAMN-HAV HAV399 |
format |
Master Thesis |
author |
Håvardstun, Susanne |
author_facet |
Håvardstun, Susanne |
author_sort |
Håvardstun, Susanne |
title |
Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels |
title_short |
Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels |
title_full |
Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels |
title_fullStr |
Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels |
title_full_unstemmed |
Triploid induction in Atlantic cod (Gadus morhua L.) by the use of different pressure levels |
title_sort |
triploid induction in atlantic cod (gadus morhua l.) by the use of different pressure levels |
publisher |
The University of Bergen |
publishDate |
2011 |
url |
http://hdl.handle.net/1956/5572 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
http://hdl.handle.net/1956/5572 |
op_rights |
Copyright the author. All rights reserved |
_version_ |
1766357406341660672 |