Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean
Sponges are known to frequently contain a great number of symbiotic organisms. Symbiosis is also a characteristic trait of vent and seep animals worldwide. Sponges in the deep sea family Cladorhizidae, otherwise also known for their surprising ability to capture invertebrate prey, are among the most...
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The University of Bergen
2009
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ftunivbergen:oai:bora.uib.no:1956/3934 2023-05-15T15:02:00+02:00 Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean Thomassen, Jon Hestetun 2009-09-30 6673686 bytes application/pdf http://hdl.handle.net/1956/3934 eng eng The University of Bergen http://hdl.handle.net/1956/3934 The author Copyright the author. All rights reserved Cladorhizidae 759906 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Master thesis 2009 ftunivbergen 2023-03-14T17:38:41Z Sponges are known to frequently contain a great number of symbiotic organisms. Symbiosis is also a characteristic trait of vent and seep animals worldwide. Sponges in the deep sea family Cladorhizidae, otherwise also known for their surprising ability to capture invertebrate prey, are among the most common sponges found in proximity to vents, and in one reported case methanotrophic symbiotic bacteria have been described in a cladorhizid species at a mud volcano. The phylogenetic relationships within the Cladorhizidae and between the cladorhizids and sister families are uncertain. New species of Cladorhizidae are still discovered, even in comparatively well studied areas such as the North Atlantic, and their affinity to vent systems make them likely candidates for harboring methanotrophic symbionts. The 2006-2009 R/V G.O. Sars" cruises to methane rich habitats in the Norwegian and Arctic Seas have yielded a number of cladorhizid specimens. This thesis contains a taxonomic inventory of these specimens using traditional morphological methods, and the results of sequencing the Folmer COI, and ITS partitions of the identified species. Lastly, the pmoA partition of the gene coding for particulate methane monooxygenase in known methanotrophic bacteria, was sequenced as a method to determine the presence of any methanotrophic symbionts from the specimens sampled. Morphological results showed that the R/V G.O. Sars" material contained six Asbestopluma and three Cladorhiza species. With the exception of two species affiliated to A. lycopodium, all were previously described species from the North Atlantic and Arctic. Though obtaining sequences proved challenging due to contamination from other organisms, cloning allowed the separation of cladorhizid and contaminant sequences, and it proved possible to get COI and ITS sequences from nearly all morphologically identified species. ITS sequences proved too divergent for phylogenetic analysis; however Folmer COI sequences had a level of resolution applicable both to place the ... Master Thesis Arctic North Atlantic University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic Folmer ENVELOPE(12.601,12.601,64.648,64.648) |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Cladorhizidae 759906 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
spellingShingle |
Cladorhizidae 759906 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Thomassen, Jon Hestetun Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean |
topic_facet |
Cladorhizidae 759906 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
description |
Sponges are known to frequently contain a great number of symbiotic organisms. Symbiosis is also a characteristic trait of vent and seep animals worldwide. Sponges in the deep sea family Cladorhizidae, otherwise also known for their surprising ability to capture invertebrate prey, are among the most common sponges found in proximity to vents, and in one reported case methanotrophic symbiotic bacteria have been described in a cladorhizid species at a mud volcano. The phylogenetic relationships within the Cladorhizidae and between the cladorhizids and sister families are uncertain. New species of Cladorhizidae are still discovered, even in comparatively well studied areas such as the North Atlantic, and their affinity to vent systems make them likely candidates for harboring methanotrophic symbionts. The 2006-2009 R/V G.O. Sars" cruises to methane rich habitats in the Norwegian and Arctic Seas have yielded a number of cladorhizid specimens. This thesis contains a taxonomic inventory of these specimens using traditional morphological methods, and the results of sequencing the Folmer COI, and ITS partitions of the identified species. Lastly, the pmoA partition of the gene coding for particulate methane monooxygenase in known methanotrophic bacteria, was sequenced as a method to determine the presence of any methanotrophic symbionts from the specimens sampled. Morphological results showed that the R/V G.O. Sars" material contained six Asbestopluma and three Cladorhiza species. With the exception of two species affiliated to A. lycopodium, all were previously described species from the North Atlantic and Arctic. Though obtaining sequences proved challenging due to contamination from other organisms, cloning allowed the separation of cladorhizid and contaminant sequences, and it proved possible to get COI and ITS sequences from nearly all morphologically identified species. ITS sequences proved too divergent for phylogenetic analysis; however Folmer COI sequences had a level of resolution applicable both to place the ... |
format |
Master Thesis |
author |
Thomassen, Jon Hestetun |
author_facet |
Thomassen, Jon Hestetun |
author_sort |
Thomassen, Jon Hestetun |
title |
Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean |
title_short |
Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean |
title_full |
Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean |
title_fullStr |
Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean |
title_full_unstemmed |
Cladorhizid sponges from hydrothermal vents and cold seeps in the NE Atlantic Ocean |
title_sort |
cladorhizid sponges from hydrothermal vents and cold seeps in the ne atlantic ocean |
publisher |
The University of Bergen |
publishDate |
2009 |
url |
http://hdl.handle.net/1956/3934 |
long_lat |
ENVELOPE(12.601,12.601,64.648,64.648) |
geographic |
Arctic Folmer |
geographic_facet |
Arctic Folmer |
genre |
Arctic North Atlantic |
genre_facet |
Arctic North Atlantic |
op_relation |
http://hdl.handle.net/1956/3934 |
op_rights |
The author Copyright the author. All rights reserved |
_version_ |
1766334009477955584 |