Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar)
The commercial importance of Atlantic salmon (Salmo salar) in aquaculture has fuelled much research into fish health since outbreaks of infectious disease cause major financial losses. All aspects of immunology are therefore currently of great interest. The aim of this study was to extend the charac...
Published in: | Developmental & Comparative Immunology |
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Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
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The University of Bergen
2009
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ftunivbergen:oai:bora.uib.no:1956/3833 2023-05-15T15:30:25+02:00 Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar) Moore, Lindsey J. 2009-03-27 application/pdf http://hdl.handle.net/1956/3833 eng eng The University of Bergen Paper I: Developmental and Comparative Immunology 32(1), Liu, Y.; Moore, L. J.; Koppang, E. O.; Hordvik, I., Characterization of the CD3ζ, CD3γδ and CD3ε subunits of the T cell receptor complex in Atlantic salmon, pp. 26-35. Copyright 2007 Elsevier Ltd. Full text not available in BORA due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1016/j.dci.2007.03.015 Paper II: Molecular Immunology 42(10), Moore, L. J.; Somamoto, T.; Lie, K. K.; Dijkstra, J. M.; Hordvik, I., Characterisation of salmon and trout CD8α and CD8β, pp. 1225-1234. Copyright 2004 Elsevier Ltd. Full text not available in BORA due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1016/j.molimm.2004.11.017 Paper III: Fish and Shellfish Immunology 26(1), Moore, L. J.; Dijkstra, J. M.; Koppang, E. O.;.Hordvik, I., CD4 Homologues in Atlantic Salmon, pp. 10-18. Copyright 2008 Elsevier Ltd. Full text not available in BORA due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1016/j.fsi.2008.09.019 Paper IV: Moore, L. J.; Koppang, E. O.; Fischer, U.; Tranulis, M.; Hordvik, I., 2009, Distribution of T cells in Atlantic salmon tissue using an antibody to CD3ε. Full text not available in BORA. urn:isbn:978-82-308-0761-3 (print version) http://hdl.handle.net/1956/3833 Lindsey J. Moore VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 Doctoral thesis 2009 ftunivbergen https://doi.org/10.1016/j.dci.2007.03.01510.1016/j.molimm.2004.11.01710.1016/j.fsi.2008.09.019 2023-03-14T17:42:24Z The commercial importance of Atlantic salmon (Salmo salar) in aquaculture has fuelled much research into fish health since outbreaks of infectious disease cause major financial losses. All aspects of immunology are therefore currently of great interest. The aim of this study was to extend the characterisation of T cell markers to enable antibody production and design of expression assays, to study the immune system and immune responses of salmon. Therefore, the genes and cDNAs for the T cell coreceptors CD3, CD8 and CD4 were cloned using a combination of synteny analysis and homology cloning. CD3 in Atlantic salmon consists of three different molecules; a γδ chain, the forerunner of separate γ and δ chains in mammals, an ε chain and a ζ chain (mammals also have an η chain, which is a splice variant of the ζ gene). The translated sequences have low identities to mammalian CD3 sequences (12-34%), but they exhibit similar characteristics with single immunoglobulin-like domains in the γδ and ε genes and immunoreceptor tyrosine-based activation motifs in all cytoplasmic domains. Two copies of the CD3ζ gene were cloned, but these are considered to be alleles. The CD3ε gene has a second copy, but it is a pseudogene containing frame-shifts and stop codons and was poorly expressed compared to the intact CD3ε gene. The CD3γδ gene also appeared to be duplicated and the variants are named CD3γδ-A and CD3γδ -B. Further evidence for the homology of CD3γδ and CD3ε genes was found in their genomic orientation where the pseudo-CD3ε gene is located tail to tail with a CD3γδ gene, similar to the gene organisation in higher vertebrates. There are two CD8 genes in Atlantic salmon, CD8α and CD8β. The synteny of these genes in humans is conserved with the publically available fugu genome sequence, allowing the subsequent identification of the CD8β gene in Atlantic salmon. There are two main transcripts and two alternative transcripts which could result in a putative truncated cytoplasmic domain in CD8α and a severely truncated version ... Doctoral or Postdoctoral Thesis Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) Developmental & Comparative Immunology 32 1 26 35 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 |
spellingShingle |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 Moore, Lindsey J. Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar) |
topic_facet |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 |
description |
The commercial importance of Atlantic salmon (Salmo salar) in aquaculture has fuelled much research into fish health since outbreaks of infectious disease cause major financial losses. All aspects of immunology are therefore currently of great interest. The aim of this study was to extend the characterisation of T cell markers to enable antibody production and design of expression assays, to study the immune system and immune responses of salmon. Therefore, the genes and cDNAs for the T cell coreceptors CD3, CD8 and CD4 were cloned using a combination of synteny analysis and homology cloning. CD3 in Atlantic salmon consists of three different molecules; a γδ chain, the forerunner of separate γ and δ chains in mammals, an ε chain and a ζ chain (mammals also have an η chain, which is a splice variant of the ζ gene). The translated sequences have low identities to mammalian CD3 sequences (12-34%), but they exhibit similar characteristics with single immunoglobulin-like domains in the γδ and ε genes and immunoreceptor tyrosine-based activation motifs in all cytoplasmic domains. Two copies of the CD3ζ gene were cloned, but these are considered to be alleles. The CD3ε gene has a second copy, but it is a pseudogene containing frame-shifts and stop codons and was poorly expressed compared to the intact CD3ε gene. The CD3γδ gene also appeared to be duplicated and the variants are named CD3γδ-A and CD3γδ -B. Further evidence for the homology of CD3γδ and CD3ε genes was found in their genomic orientation where the pseudo-CD3ε gene is located tail to tail with a CD3γδ gene, similar to the gene organisation in higher vertebrates. There are two CD8 genes in Atlantic salmon, CD8α and CD8β. The synteny of these genes in humans is conserved with the publically available fugu genome sequence, allowing the subsequent identification of the CD8β gene in Atlantic salmon. There are two main transcripts and two alternative transcripts which could result in a putative truncated cytoplasmic domain in CD8α and a severely truncated version ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Moore, Lindsey J. |
author_facet |
Moore, Lindsey J. |
author_sort |
Moore, Lindsey J. |
title |
Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar) |
title_short |
Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar) |
title_full |
Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar) |
title_fullStr |
Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar) |
title_full_unstemmed |
Molecular characterisaton of T cell co-receptors CD3, CD8 and CD4 in Atlantic salmon (Salmo salar) |
title_sort |
molecular characterisaton of t cell co-receptors cd3, cd8 and cd4 in atlantic salmon (salmo salar) |
publisher |
The University of Bergen |
publishDate |
2009 |
url |
http://hdl.handle.net/1956/3833 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
Paper I: Developmental and Comparative Immunology 32(1), Liu, Y.; Moore, L. J.; Koppang, E. O.; Hordvik, I., Characterization of the CD3ζ, CD3γδ and CD3ε subunits of the T cell receptor complex in Atlantic salmon, pp. 26-35. Copyright 2007 Elsevier Ltd. Full text not available in BORA due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1016/j.dci.2007.03.015 Paper II: Molecular Immunology 42(10), Moore, L. J.; Somamoto, T.; Lie, K. K.; Dijkstra, J. M.; Hordvik, I., Characterisation of salmon and trout CD8α and CD8β, pp. 1225-1234. Copyright 2004 Elsevier Ltd. Full text not available in BORA due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1016/j.molimm.2004.11.017 Paper III: Fish and Shellfish Immunology 26(1), Moore, L. J.; Dijkstra, J. M.; Koppang, E. O.;.Hordvik, I., CD4 Homologues in Atlantic Salmon, pp. 10-18. Copyright 2008 Elsevier Ltd. Full text not available in BORA due to publisher restrictions. The published version is available at: http://dx.doi.org/10.1016/j.fsi.2008.09.019 Paper IV: Moore, L. J.; Koppang, E. O.; Fischer, U.; Tranulis, M.; Hordvik, I., 2009, Distribution of T cells in Atlantic salmon tissue using an antibody to CD3ε. Full text not available in BORA. urn:isbn:978-82-308-0761-3 (print version) http://hdl.handle.net/1956/3833 |
op_rights |
Lindsey J. Moore |
op_doi |
https://doi.org/10.1016/j.dci.2007.03.01510.1016/j.molimm.2004.11.01710.1016/j.fsi.2008.09.019 |
container_title |
Developmental & Comparative Immunology |
container_volume |
32 |
container_issue |
1 |
container_start_page |
26 |
op_container_end_page |
35 |
_version_ |
1766360872898265088 |