Spatial transcription of CYP1A in fish liver
Background: The aim of this work was to study how evenly detoxifying genes are transcribed spatially in liver tissue of fish. Ten Atlantic salmon Salmo salar were intraperitoneally injected with 50 mg/kg of the strong CYP1A inducer β-naphthoflavone and liver tissue harvested seven days later. The li...
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Online Access: | http://hdl.handle.net/1956/2675 https://doi.org/10.1186/1472-6793-7-12 |
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ftunivbergen:oai:bora.uib.no:1956/2675 2023-05-15T15:31:56+02:00 Spatial transcription of CYP1A in fish liver Olsvik, Pål A. Lie, Kai K. Sæle, Øystein Sanden, Monica 2007-12-05 application/pdf http://hdl.handle.net/1956/2675 https://doi.org/10.1186/1472-6793-7-12 eng eng BioMed Central urn:issn:1472-6793 http://hdl.handle.net/1956/2675 https://doi.org/10.1186/1472-6793-7-12 VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471 Peer reviewed Journal article 2007 ftunivbergen https://doi.org/10.1186/1472-6793-7-12 2023-03-14T17:43:00Z Background: The aim of this work was to study how evenly detoxifying genes are transcribed spatially in liver tissue of fish. Ten Atlantic salmon Salmo salar were intraperitoneally injected with 50 mg/kg of the strong CYP1A inducer β-naphthoflavone and liver tissue harvested seven days later. The liver from 10 control and 10 exposed fish were split into eight sections, RNA extracted and three reference (β-actin, elongation factor 1AB (EF1AB)) and two detoxifying genes (CYP1A and GST) quantified with real-time RT-PCR. The cellular localization of the EF1AB and CYP1A mRNA in the liver of control and β-naphthoflavone treated fish was then determined by in situ hybridization (ISH) using EF1AB and CYP1A biotinylated oligonucleotide probes. Results: The study shows that genes encoding phase I and phase II conjugating enzymes are unevenly transcribed in different parts of the liver of Atlantic salmon seven days after a single-dose of β-naphthoflavone exposure. Transcription of CYP1A and GST was higher in the middle section of the liver compared to the distal and proximal parts of the organ. The ISH data suggest that CYP1A transcription happens mainly in hepatocyte cells in the liver, and that hepatocytes in the vicinity of blood vessels respond stronger to β-naphthoflavone than cells further away from the blood supply. Conclusion: Overall, the qRT-PCR and ISH results reported here suggest that gene expression analysis should be performed on as pure cell populations as possible. If bulk tissue samples are to be used, one should always check how evenly the target genes are expressed in tissue sections and organs in every study. Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) BMC Physiology 7 1 12 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471 |
spellingShingle |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471 Olsvik, Pål A. Lie, Kai K. Sæle, Øystein Sanden, Monica Spatial transcription of CYP1A in fish liver |
topic_facet |
VDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920::Fiskehelse: 923 VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471 |
description |
Background: The aim of this work was to study how evenly detoxifying genes are transcribed spatially in liver tissue of fish. Ten Atlantic salmon Salmo salar were intraperitoneally injected with 50 mg/kg of the strong CYP1A inducer β-naphthoflavone and liver tissue harvested seven days later. The liver from 10 control and 10 exposed fish were split into eight sections, RNA extracted and three reference (β-actin, elongation factor 1AB (EF1AB)) and two detoxifying genes (CYP1A and GST) quantified with real-time RT-PCR. The cellular localization of the EF1AB and CYP1A mRNA in the liver of control and β-naphthoflavone treated fish was then determined by in situ hybridization (ISH) using EF1AB and CYP1A biotinylated oligonucleotide probes. Results: The study shows that genes encoding phase I and phase II conjugating enzymes are unevenly transcribed in different parts of the liver of Atlantic salmon seven days after a single-dose of β-naphthoflavone exposure. Transcription of CYP1A and GST was higher in the middle section of the liver compared to the distal and proximal parts of the organ. The ISH data suggest that CYP1A transcription happens mainly in hepatocyte cells in the liver, and that hepatocytes in the vicinity of blood vessels respond stronger to β-naphthoflavone than cells further away from the blood supply. Conclusion: Overall, the qRT-PCR and ISH results reported here suggest that gene expression analysis should be performed on as pure cell populations as possible. If bulk tissue samples are to be used, one should always check how evenly the target genes are expressed in tissue sections and organs in every study. |
format |
Article in Journal/Newspaper |
author |
Olsvik, Pål A. Lie, Kai K. Sæle, Øystein Sanden, Monica |
author_facet |
Olsvik, Pål A. Lie, Kai K. Sæle, Øystein Sanden, Monica |
author_sort |
Olsvik, Pål A. |
title |
Spatial transcription of CYP1A in fish liver |
title_short |
Spatial transcription of CYP1A in fish liver |
title_full |
Spatial transcription of CYP1A in fish liver |
title_fullStr |
Spatial transcription of CYP1A in fish liver |
title_full_unstemmed |
Spatial transcription of CYP1A in fish liver |
title_sort |
spatial transcription of cyp1a in fish liver |
publisher |
BioMed Central |
publishDate |
2007 |
url |
http://hdl.handle.net/1956/2675 https://doi.org/10.1186/1472-6793-7-12 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
urn:issn:1472-6793 http://hdl.handle.net/1956/2675 https://doi.org/10.1186/1472-6793-7-12 |
op_doi |
https://doi.org/10.1186/1472-6793-7-12 |
container_title |
BMC Physiology |
container_volume |
7 |
container_issue |
1 |
container_start_page |
12 |
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1766362427144798208 |