Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge
A bacterial strain, designated BAR1T, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1T were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10–37 °C (optimum 27–35 °C), pH 5.5...
Published in: | International Journal of Systematic and Evolutionary Microbiology |
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Language: | English |
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2020
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Online Access: | https://hdl.handle.net/1956/23504 https://doi.org/10.1099/ijsem.0.003234 |
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ftunivbergen:oai:bora.uib.no:1956/23504 2023-05-15T15:08:33+02:00 Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge Le Moine Bauer, Sven Sjøberg, Andreas Gilje L´Haridon, Stephane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon 2020-01-02T12:29:49Z application/pdf https://hdl.handle.net/1956/23504 https://doi.org/10.1099/ijsem.0.003234 eng eng Microbiology Society urn:issn:1466-5034 urn:issn:1466-5026 https://hdl.handle.net/1956/23504 https://doi.org/10.1099/ijsem.0.003234 cristin:1677450 Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2019 IUMS International Journal of Systematic and Evolutionary Microbiology Peer reviewed Journal article 2020 ftunivbergen https://doi.org/10.1099/ijsem.0.003234 2023-03-14T17:41:20Z A bacterial strain, designated BAR1T, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1T were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10–37 °C (optimum 27–35 °C), pH 5.5–8.0 (optimum pH 6.5–7.5) and 0.5–5.0 % NaCl (optimum 2 %). The DNA G+C content was 57.38 mol%. The membrane-associated major ubiquinone was Q-10, the fatty acid profile was dominated by C18 : 1ω7c (91 %), and the polar lipids detected were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, one unidentified lipid and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BAR1T clustered together with Rhodobacterales bacterium PRT1, as well as the genera Halocynthiibacter and Pseudohalocynthiibacter in a polyphyletic clade within the Roseobacter clade. Several characteristics differentiate strain BAR1T from the aforementioned genera, including its motility, its piezophilic behaviour and its ability to grow at 35 °C and under anaerobic conditions. Accordingly, strain BAR1T is considered to represent a novel genus and species within the Roseobacter clade, for which the name Profundibacter amoris gen. nov., sp. nov. is proposed. The type strain is Profundibacter amoris BAR1T (=JCM 31874T=DSM 104147T). acceptedVersion Article in Journal/Newspaper Arctic University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic International Journal of Systematic and Evolutionary Microbiology 69 4 975 981 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
A bacterial strain, designated BAR1T, was isolated from a microbial mat growing on the surface of a barite chimney at the Loki’s Castle Vent Field, at a depth of 2216 m. Cells of strain BAR1T were rod-shaped, Gram-reaction-negative and grew on marine broth 2216 at 10–37 °C (optimum 27–35 °C), pH 5.5–8.0 (optimum pH 6.5–7.5) and 0.5–5.0 % NaCl (optimum 2 %). The DNA G+C content was 57.38 mol%. The membrane-associated major ubiquinone was Q-10, the fatty acid profile was dominated by C18 : 1ω7c (91 %), and the polar lipids detected were phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, one unidentified lipid and one unidentified phospholipid. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain BAR1T clustered together with Rhodobacterales bacterium PRT1, as well as the genera Halocynthiibacter and Pseudohalocynthiibacter in a polyphyletic clade within the Roseobacter clade. Several characteristics differentiate strain BAR1T from the aforementioned genera, including its motility, its piezophilic behaviour and its ability to grow at 35 °C and under anaerobic conditions. Accordingly, strain BAR1T is considered to represent a novel genus and species within the Roseobacter clade, for which the name Profundibacter amoris gen. nov., sp. nov. is proposed. The type strain is Profundibacter amoris BAR1T (=JCM 31874T=DSM 104147T). acceptedVersion |
format |
Article in Journal/Newspaper |
author |
Le Moine Bauer, Sven Sjøberg, Andreas Gilje L´Haridon, Stephane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon |
spellingShingle |
Le Moine Bauer, Sven Sjøberg, Andreas Gilje L´Haridon, Stephane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
author_facet |
Le Moine Bauer, Sven Sjøberg, Andreas Gilje L´Haridon, Stephane Stokke, Runar Roalkvam, Irene Steen, Ida Helene Dahle, Håkon |
author_sort |
Le Moine Bauer, Sven |
title |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_short |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_full |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_fullStr |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_full_unstemmed |
Profundibacter amoris gen. nov., sp. nov., a new member of the Roseobacter clade isolated from Loki's Castle Vent Field on the Arctic Mid-Ocean Ridge |
title_sort |
profundibacter amoris gen. nov., sp. nov., a new member of the roseobacter clade isolated from loki's castle vent field on the arctic mid-ocean ridge |
publisher |
Microbiology Society |
publishDate |
2020 |
url |
https://hdl.handle.net/1956/23504 https://doi.org/10.1099/ijsem.0.003234 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
International Journal of Systematic and Evolutionary Microbiology |
op_relation |
urn:issn:1466-5034 urn:issn:1466-5026 https://hdl.handle.net/1956/23504 https://doi.org/10.1099/ijsem.0.003234 cristin:1677450 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2019 IUMS |
op_doi |
https://doi.org/10.1099/ijsem.0.003234 |
container_title |
International Journal of Systematic and Evolutionary Microbiology |
container_volume |
69 |
container_issue |
4 |
container_start_page |
975 |
op_container_end_page |
981 |
_version_ |
1766339893070397440 |