Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances
The dopaminergic effect of PAH and PFAS mixtures, prepared according to environmentally relevant concentrations, has been studied in juvenile female Atlantic cod (Gadus morhua). Benzo[a]pyrene, dibenzothiophene, fluorene, naphthalene, phenanthrene, and pyrene were used to prepare a PAH mixture, whil...
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Online Access: | http://hdl.handle.net/1956/22362 https://doi.org/10.1021/acs.est.9b00637 |
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ftunivbergen:oai:bora.uib.no:1956/22362 2023-05-15T15:27:14+02:00 Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances Khan, Essa Ahsan Bertotto, Luisa B. Dale, Karina Lille-Langøy, Roger Yadetie, Fekadu Karlsen, Odd Andre Goksøyr, Anders Schlenk, Daniel Arukwe, Augustine 2020-02-10T08:13:10Z application/pdf http://hdl.handle.net/1956/22362 https://doi.org/10.1021/acs.est.9b00637 eng eng ACS Norges forskningsråd: 248840 urn:issn:1520-5851 urn:issn:0013-936X http://hdl.handle.net/1956/22362 https://doi.org/10.1021/acs.est.9b00637 cristin:1709933 Copyright 2019 American Chemical Society Environmental Science and Technology Peer reviewed Journal article 2020 ftunivbergen https://doi.org/10.1021/acs.est.9b00637 2023-03-14T17:42:37Z The dopaminergic effect of PAH and PFAS mixtures, prepared according to environmentally relevant concentrations, has been studied in juvenile female Atlantic cod (Gadus morhua). Benzo[a]pyrene, dibenzothiophene, fluorene, naphthalene, phenanthrene, and pyrene were used to prepare a PAH mixture, while PFNA, PFOA, PFOS, and PFTrA were used to prepare a PFAS mixture. Cod were injected intraperitoneally twice, with either a low (1×) or high (20×) dose of each compound mixture or their combinations. After 2 weeks of exposure, levels of plasma 17β-estradiol (E2) were significantly elevated in high PAH/high PFAS treated group. Brain dopamine/metabolite ratios (DOPAC/dopamine and HVA+DOPAC/dopamine) changed with E2 plasma levels, except for high PAH/low PFAS and low PAH/high PFAS treated groups. On the transcript levels, th mRNA inversely correlated with dopamine/metabolite ratios and gnrh2 mRNA levels. Respective decreases and increases of drd1 and drd2a after exposure to the high PAH dose were observed. Specifically, high PFAS exposure decreased both drds, leading to high plasma E2 concentrations. Other studied end points suggest that these compounds, at different doses and combinations, have different toxicity threshold and modes of action. These effects indicate potential alterations in the feedback signaling processes within the dopaminergic pathway by these contaminant mixtures. acceptedVersion Article in Journal/Newspaper atlantic cod Gadus morhua University of Bergen: Bergen Open Research Archive (BORA-UiB) Environmental Science & Technology 53 12 7036 7044 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
The dopaminergic effect of PAH and PFAS mixtures, prepared according to environmentally relevant concentrations, has been studied in juvenile female Atlantic cod (Gadus morhua). Benzo[a]pyrene, dibenzothiophene, fluorene, naphthalene, phenanthrene, and pyrene were used to prepare a PAH mixture, while PFNA, PFOA, PFOS, and PFTrA were used to prepare a PFAS mixture. Cod were injected intraperitoneally twice, with either a low (1×) or high (20×) dose of each compound mixture or their combinations. After 2 weeks of exposure, levels of plasma 17β-estradiol (E2) were significantly elevated in high PAH/high PFAS treated group. Brain dopamine/metabolite ratios (DOPAC/dopamine and HVA+DOPAC/dopamine) changed with E2 plasma levels, except for high PAH/low PFAS and low PAH/high PFAS treated groups. On the transcript levels, th mRNA inversely correlated with dopamine/metabolite ratios and gnrh2 mRNA levels. Respective decreases and increases of drd1 and drd2a after exposure to the high PAH dose were observed. Specifically, high PFAS exposure decreased both drds, leading to high plasma E2 concentrations. Other studied end points suggest that these compounds, at different doses and combinations, have different toxicity threshold and modes of action. These effects indicate potential alterations in the feedback signaling processes within the dopaminergic pathway by these contaminant mixtures. acceptedVersion |
format |
Article in Journal/Newspaper |
author |
Khan, Essa Ahsan Bertotto, Luisa B. Dale, Karina Lille-Langøy, Roger Yadetie, Fekadu Karlsen, Odd Andre Goksøyr, Anders Schlenk, Daniel Arukwe, Augustine |
spellingShingle |
Khan, Essa Ahsan Bertotto, Luisa B. Dale, Karina Lille-Langøy, Roger Yadetie, Fekadu Karlsen, Odd Andre Goksøyr, Anders Schlenk, Daniel Arukwe, Augustine Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances |
author_facet |
Khan, Essa Ahsan Bertotto, Luisa B. Dale, Karina Lille-Langøy, Roger Yadetie, Fekadu Karlsen, Odd Andre Goksøyr, Anders Schlenk, Daniel Arukwe, Augustine |
author_sort |
Khan, Essa Ahsan |
title |
Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances |
title_short |
Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances |
title_full |
Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances |
title_fullStr |
Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances |
title_full_unstemmed |
Modulation of neuro-dopamine homeostasis in juvenile female Atlantic cod (Gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances |
title_sort |
modulation of neuro-dopamine homeostasis in juvenile female atlantic cod (gadus morhua) exposed to polycyclic aromatic hydrocarbons and perfluoroalkyl substances |
publisher |
ACS |
publishDate |
2020 |
url |
http://hdl.handle.net/1956/22362 https://doi.org/10.1021/acs.est.9b00637 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Environmental Science and Technology |
op_relation |
Norges forskningsråd: 248840 urn:issn:1520-5851 urn:issn:0013-936X http://hdl.handle.net/1956/22362 https://doi.org/10.1021/acs.est.9b00637 cristin:1709933 |
op_rights |
Copyright 2019 American Chemical Society |
op_doi |
https://doi.org/10.1021/acs.est.9b00637 |
container_title |
Environmental Science & Technology |
container_volume |
53 |
container_issue |
12 |
container_start_page |
7036 |
op_container_end_page |
7044 |
_version_ |
1766357682488344576 |