Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice
A large fraction of the n-3 polyunsaturated fatty acids (PUFAs) in cod fillet is present in the form of phospholipids (PLs). Freezing initiates hydrolysis of the PLs present in the fillet. Here, we compared the effects of Western diets based on frozen cod, fresh cod or pork with a diet based on case...
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ftunivbergen:oai:bora.uib.no:1956/18887 2023-05-15T15:27:41+02:00 Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice Fauske, Kristin Røen Bernhard, Annette Fjære, Even Myrmel, Lene Secher Frøyland, Livar Kristiansen, Karsten Liaset, Bjørn Madsen, Lise 2018-05-30 application/pdf https://hdl.handle.net/1956/18887 https://doi.org/10.3390/nu10060695 eng eng MDPI urn:issn:2072-6643 https://hdl.handle.net/1956/18887 https://doi.org/10.3390/nu10060695 cristin:1593671 Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2018 The Author(s) 695 Nutrients 10 6 Obesity Western diet cod pork free fatty acids phospholipids triacylglycerol n-3 polyunsaturated fatty acids mice Peer reviewed Journal article 2018 ftunivbergen https://doi.org/10.3390/nu10060695 2023-03-14T17:39:31Z A large fraction of the n-3 polyunsaturated fatty acids (PUFAs) in cod fillet is present in the form of phospholipids (PLs). Freezing initiates hydrolysis of the PLs present in the fillet. Here, we compared the effects of Western diets based on frozen cod, fresh cod or pork with a diet based on casein in male C57BL/6J mice fed for 12 weeks at thermoneutrality. Diets based on fresh cod contained more PL-bound n-3 PUFAs (3.12 mg/g diet) than diets based on frozen cod (1.9 mg/g diet). Mice fed diets containing pork and fresh cod, but not frozen cod, gained more body and fat mass than casein-fed mice. Additionally, the bioavailability of n-3 PUFAs present in the cod fillets was not influenced by storage conditions. In a second experiment, diets with pork as the protein source were supplemented with n-3 PUFAs in the form of PL or triacylglycerol (TAG) to match the levels of the diet containing fresh cod. Adding PL-bound, but not TAG-bound, n-3 PUFAs, to the pork-based diet increased body and fat mass gain. Thus, supplementation with PL-bound n-3 PUFAs did not protect against, but rather promoted, obesity development in mice fed a pork-based diet. publishedVersion Article in Journal/Newspaper atlantic cod University of Bergen: Bergen Open Research Archive (BORA-UiB) Nutrients 10 6 695 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Obesity Western diet cod pork free fatty acids phospholipids triacylglycerol n-3 polyunsaturated fatty acids mice |
spellingShingle |
Obesity Western diet cod pork free fatty acids phospholipids triacylglycerol n-3 polyunsaturated fatty acids mice Fauske, Kristin Røen Bernhard, Annette Fjære, Even Myrmel, Lene Secher Frøyland, Livar Kristiansen, Karsten Liaset, Bjørn Madsen, Lise Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice |
topic_facet |
Obesity Western diet cod pork free fatty acids phospholipids triacylglycerol n-3 polyunsaturated fatty acids mice |
description |
A large fraction of the n-3 polyunsaturated fatty acids (PUFAs) in cod fillet is present in the form of phospholipids (PLs). Freezing initiates hydrolysis of the PLs present in the fillet. Here, we compared the effects of Western diets based on frozen cod, fresh cod or pork with a diet based on casein in male C57BL/6J mice fed for 12 weeks at thermoneutrality. Diets based on fresh cod contained more PL-bound n-3 PUFAs (3.12 mg/g diet) than diets based on frozen cod (1.9 mg/g diet). Mice fed diets containing pork and fresh cod, but not frozen cod, gained more body and fat mass than casein-fed mice. Additionally, the bioavailability of n-3 PUFAs present in the cod fillets was not influenced by storage conditions. In a second experiment, diets with pork as the protein source were supplemented with n-3 PUFAs in the form of PL or triacylglycerol (TAG) to match the levels of the diet containing fresh cod. Adding PL-bound, but not TAG-bound, n-3 PUFAs, to the pork-based diet increased body and fat mass gain. Thus, supplementation with PL-bound n-3 PUFAs did not protect against, but rather promoted, obesity development in mice fed a pork-based diet. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Fauske, Kristin Røen Bernhard, Annette Fjære, Even Myrmel, Lene Secher Frøyland, Livar Kristiansen, Karsten Liaset, Bjørn Madsen, Lise |
author_facet |
Fauske, Kristin Røen Bernhard, Annette Fjære, Even Myrmel, Lene Secher Frøyland, Livar Kristiansen, Karsten Liaset, Bjørn Madsen, Lise |
author_sort |
Fauske, Kristin Røen |
title |
Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice |
title_short |
Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice |
title_full |
Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice |
title_fullStr |
Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice |
title_full_unstemmed |
Effects of Frozen Storage on Phospholipid Content in Atlantic Cod Fillets and the Influence on Diet-Induced Obesity in Mice |
title_sort |
effects of frozen storage on phospholipid content in atlantic cod fillets and the influence on diet-induced obesity in mice |
publisher |
MDPI |
publishDate |
2018 |
url |
https://hdl.handle.net/1956/18887 https://doi.org/10.3390/nu10060695 |
genre |
atlantic cod |
genre_facet |
atlantic cod |
op_source |
695 Nutrients 10 6 |
op_relation |
urn:issn:2072-6643 https://hdl.handle.net/1956/18887 https://doi.org/10.3390/nu10060695 cristin:1593671 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2018 The Author(s) |
op_doi |
https://doi.org/10.3390/nu10060695 |
container_title |
Nutrients |
container_volume |
10 |
container_issue |
6 |
container_start_page |
695 |
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1766358099686326272 |