Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts
Mouthrot, caused by Tenacibaculum maritimum is a significant disease of farmed Atlantic salmon, Salmo salar on the West Coast of North America. Smolts recently transferred into saltwater are the most susceptible and affected fish die with little internal or external clinical signs other than the cha...
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ftunivbergen:oai:bora.uib.no:1956/18883 2023-05-15T15:31:40+02:00 Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts Frisch, Kathleen Marie Småge, Sverre Bang Johansen, Renate Duesund, Henrik Brevik, Øyvind Jakobsen Nylund, Are 2018-11-01 application/pdf https://hdl.handle.net/1956/18883 https://doi.org/10.1371/journal.pone.0206951 eng eng PLoS urn:issn:1932-6203 https://hdl.handle.net/1956/18883 https://doi.org/10.1371/journal.pone.0206951 Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2018 The Author(s) e0206951 PloS ONE 13 11 Peer reviewed Journal article 2018 ftunivbergen https://doi.org/10.1371/journal.pone.0206951 2023-03-14T17:39:57Z Mouthrot, caused by Tenacibaculum maritimum is a significant disease of farmed Atlantic salmon, Salmo salar on the West Coast of North America. Smolts recently transferred into saltwater are the most susceptible and affected fish die with little internal or external clinical signs other than the characteristic small (usually < 5 mm) yellow plaques that are present inside the mouth. The mechanism by which these smolts die is unknown. This study investigated the microscopic pathology (histology and scanning electron microscopy) of bath infected smolts with Western Canadian T. maritimum isolates TmarCan15-1, TmarCan16-1 and TmarCan16-5 and compared the findings to what is seen in a natural outbreak of mouthrot. A real-time RT-PCR assay based on the outer membrane protein A specific for T. maritimum was designed and used to investigate the tissue tropism of the bacteria. The results from this showed that T. maritimum is detectable internally by real-time RT-PCR. This combined with the fact that the bacteria can be isolated from the kidney suggests that T. maritimum becomes systemic. The pathology in the infected smolts is primarily mouth lesions, including damaged tissues surrounding the teeth; the disease is similar to periodontal disease in mammals. The pathological changes are focal, severe, and occur very rapidly with little associated inflammation. Skin lesions are more common in experimentally infected smolts than in natural outbreaks, but this could be an artefact of the challenge dose, handling and tank used during the experiments. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) PLOS ONE 13 11 e0206951 |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
language |
English |
description |
Mouthrot, caused by Tenacibaculum maritimum is a significant disease of farmed Atlantic salmon, Salmo salar on the West Coast of North America. Smolts recently transferred into saltwater are the most susceptible and affected fish die with little internal or external clinical signs other than the characteristic small (usually < 5 mm) yellow plaques that are present inside the mouth. The mechanism by which these smolts die is unknown. This study investigated the microscopic pathology (histology and scanning electron microscopy) of bath infected smolts with Western Canadian T. maritimum isolates TmarCan15-1, TmarCan16-1 and TmarCan16-5 and compared the findings to what is seen in a natural outbreak of mouthrot. A real-time RT-PCR assay based on the outer membrane protein A specific for T. maritimum was designed and used to investigate the tissue tropism of the bacteria. The results from this showed that T. maritimum is detectable internally by real-time RT-PCR. This combined with the fact that the bacteria can be isolated from the kidney suggests that T. maritimum becomes systemic. The pathology in the infected smolts is primarily mouth lesions, including damaged tissues surrounding the teeth; the disease is similar to periodontal disease in mammals. The pathological changes are focal, severe, and occur very rapidly with little associated inflammation. Skin lesions are more common in experimentally infected smolts than in natural outbreaks, but this could be an artefact of the challenge dose, handling and tank used during the experiments. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Frisch, Kathleen Marie Småge, Sverre Bang Johansen, Renate Duesund, Henrik Brevik, Øyvind Jakobsen Nylund, Are |
spellingShingle |
Frisch, Kathleen Marie Småge, Sverre Bang Johansen, Renate Duesund, Henrik Brevik, Øyvind Jakobsen Nylund, Are Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts |
author_facet |
Frisch, Kathleen Marie Småge, Sverre Bang Johansen, Renate Duesund, Henrik Brevik, Øyvind Jakobsen Nylund, Are |
author_sort |
Frisch, Kathleen Marie |
title |
Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts |
title_short |
Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts |
title_full |
Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts |
title_fullStr |
Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts |
title_full_unstemmed |
Pathology of experimentally induced mouthrot caused by Tenacibaculum maritimum in Atlantic salmon smolts |
title_sort |
pathology of experimentally induced mouthrot caused by tenacibaculum maritimum in atlantic salmon smolts |
publisher |
PLoS |
publishDate |
2018 |
url |
https://hdl.handle.net/1956/18883 https://doi.org/10.1371/journal.pone.0206951 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
e0206951 PloS ONE 13 11 |
op_relation |
urn:issn:1932-6203 https://hdl.handle.net/1956/18883 https://doi.org/10.1371/journal.pone.0206951 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2018 The Author(s) |
op_doi |
https://doi.org/10.1371/journal.pone.0206951 |
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PLOS ONE |
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13 |
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11 |
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e0206951 |
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1766362189813252096 |