Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses
One of the most abundant archaeal groups on Earth is the Thaumarchaeota. They are recognized as major contributors to marine ammonia oxidation, a crucial step in the biogeochemical cycling of nitrogen. Their universal success is attributed to a high genomic flexibility and niche adaptability. Based...
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ftunivbergen:oai:bora.uib.no:1956/18529 2023-05-15T14:24:02+02:00 Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses Müller, Oliver Wilson, Bryan Paulsen, Maria Lund Rumińska, Agnieszka Armo, Hilde R. Bratbak, Gunnar Øverås, Lise 2018-01-23 application/pdf https://hdl.handle.net/1956/18529 https://doi.org/10.3389/fmicb.2018.00024 eng eng Frontiers Implications of a changing Arctic on microbial communities: Following the effects of thawing permafrost from land to sea urn:issn:1664-302X https://hdl.handle.net/1956/18529 https://doi.org/10.3389/fmicb.2018.00024 Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ Copyright the Author(s) 24 Frontiers in Microbiology 9 VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 Peer reviewed Journal article 2018 ftunivbergen https://doi.org/10.3389/fmicb.2018.00024 2023-03-14T17:39:39Z One of the most abundant archaeal groups on Earth is the Thaumarchaeota. They are recognized as major contributors to marine ammonia oxidation, a crucial step in the biogeochemical cycling of nitrogen. Their universal success is attributed to a high genomic flexibility and niche adaptability. Based on differences in the gene coding for ammonia monooxygenase subunit A (amoA), two different ecotypes with distinct distribution patterns in the water column have been identified. We used high-throughput sequencing of 16S rRNA genes combined with archaeal amoA functional gene clone libraries to investigate which environmental factors are driving the distribution of Thaumarchaeota ecotypes in the Atlantic gateway to the Arctic Ocean through an annual cycle in 2014. We observed the characteristic vertical pattern of Thaumarchaeota abundance with high values in the mesopelagic (>200 m) water throughout the entire year, but also in the epipelagic (<200 m) water during the dark winter months (January, March and November). The Thaumarchaeota community was dominated by three OTUs which on average comprised 76% ± 11 and varied in relative abundance according to water mass characteristics and not to depth or ammonium concentration, as suggested in previous studies. The ratios of the abundance of the different OTU types were similar to that of the functional amoA water cluster types. Together, this suggests a strong selection of ecotypes within different water masses, supporting the general idea of water mass characteristics as an important factor in defining microbial community structure. If indeed, as suggested in this study, Thaumarchaeota population dynamics are controlled by a set of factors, described here as water mass characteristics and not just depth alone, then changes in water mass flow will inevitably affect the distribution of the different ecotypes. publishedVersion Article in Journal/Newspaper Arctic Arctic Arctic Ocean University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic Arctic Ocean Frontiers in Microbiology 9 |
institution |
Open Polar |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 |
spellingShingle |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 Müller, Oliver Wilson, Bryan Paulsen, Maria Lund Rumińska, Agnieszka Armo, Hilde R. Bratbak, Gunnar Øverås, Lise Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses |
topic_facet |
VDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 |
description |
One of the most abundant archaeal groups on Earth is the Thaumarchaeota. They are recognized as major contributors to marine ammonia oxidation, a crucial step in the biogeochemical cycling of nitrogen. Their universal success is attributed to a high genomic flexibility and niche adaptability. Based on differences in the gene coding for ammonia monooxygenase subunit A (amoA), two different ecotypes with distinct distribution patterns in the water column have been identified. We used high-throughput sequencing of 16S rRNA genes combined with archaeal amoA functional gene clone libraries to investigate which environmental factors are driving the distribution of Thaumarchaeota ecotypes in the Atlantic gateway to the Arctic Ocean through an annual cycle in 2014. We observed the characteristic vertical pattern of Thaumarchaeota abundance with high values in the mesopelagic (>200 m) water throughout the entire year, but also in the epipelagic (<200 m) water during the dark winter months (January, March and November). The Thaumarchaeota community was dominated by three OTUs which on average comprised 76% ± 11 and varied in relative abundance according to water mass characteristics and not to depth or ammonium concentration, as suggested in previous studies. The ratios of the abundance of the different OTU types were similar to that of the functional amoA water cluster types. Together, this suggests a strong selection of ecotypes within different water masses, supporting the general idea of water mass characteristics as an important factor in defining microbial community structure. If indeed, as suggested in this study, Thaumarchaeota population dynamics are controlled by a set of factors, described here as water mass characteristics and not just depth alone, then changes in water mass flow will inevitably affect the distribution of the different ecotypes. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Müller, Oliver Wilson, Bryan Paulsen, Maria Lund Rumińska, Agnieszka Armo, Hilde R. Bratbak, Gunnar Øverås, Lise |
author_facet |
Müller, Oliver Wilson, Bryan Paulsen, Maria Lund Rumińska, Agnieszka Armo, Hilde R. Bratbak, Gunnar Øverås, Lise |
author_sort |
Müller, Oliver |
title |
Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses |
title_short |
Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses |
title_full |
Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses |
title_fullStr |
Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses |
title_full_unstemmed |
Spatiotemporal Dynamics of Ammonia-Oxidizing Thaumarchaeota in Distinct Arctic Water Masses |
title_sort |
spatiotemporal dynamics of ammonia-oxidizing thaumarchaeota in distinct arctic water masses |
publisher |
Frontiers |
publishDate |
2018 |
url |
https://hdl.handle.net/1956/18529 https://doi.org/10.3389/fmicb.2018.00024 |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Arctic Ocean |
op_source |
24 Frontiers in Microbiology 9 |
op_relation |
Implications of a changing Arctic on microbial communities: Following the effects of thawing permafrost from land to sea urn:issn:1664-302X https://hdl.handle.net/1956/18529 https://doi.org/10.3389/fmicb.2018.00024 |
op_rights |
Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/ Copyright the Author(s) |
op_doi |
https://doi.org/10.3389/fmicb.2018.00024 |
container_title |
Frontiers in Microbiology |
container_volume |
9 |
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1766296498308382720 |