Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors
Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase i...
Published in: | Frontiers in Plant Science |
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Online Access: | https://hdl.handle.net/1956/17720 https://doi.org/10.3389/fpls.2018.00491 |
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ftunivbergen:oai:bora.uib.no:1956/17720 2023-05-15T14:51:36+02:00 Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors Steinrücken, Pia Mjøs, Svein Are Prestegard, Siv Kristin Erga, Svein Rune 2018-05-28T06:54:29Z application/pdf https://hdl.handle.net/1956/17720 https://doi.org/10.3389/fpls.2018.00491 eng eng Frontiers High-value fatty acids from microalgae. Bioprospecting and outdoor cultivation at northern latitudes urn:issn:1664-462X https://hdl.handle.net/1956/17720 https://doi.org/10.3389/fpls.2018.00491 cristin:1579768 Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2018 The Author(s) Eicosapentaenoic acid (EPA) arctic diatom factorial design Salinity growth phase interactive effects microalgal biotechnology Peer reviewed Journal article 2018 ftunivbergen https://doi.org/10.3389/fpls.2018.00491 2023-03-14T17:45:02Z Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase its EPA content from 3.0 to 4.6% of dry weight (DW) in the nutrient-replete exponential phase and nutrient-depleted stationary phase, respectively. In the present study, a factorial-design experiment was used, to investigate this effect in more detail and in combination with varying salinities and irradiances. A mathematical model revealed that both growth phase and salinity, alone and in combination, influenced the EPA content significantly. Maximum EPA values of 7.1% DW were obtained at a salinity of 22 and after 5 days in stationary phase, and might be related to a decreased silica content, an accumulation of storage lipids containing EPA, or both. However, growth rates were lower for low salinity (0.54 and 0.57 d−1) than high salinity (0.77 and 0.98 d−1) cultures. publishedVersion Article in Journal/Newspaper Arctic University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic Frontiers in Plant Science 9 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Eicosapentaenoic acid (EPA) arctic diatom factorial design Salinity growth phase interactive effects microalgal biotechnology |
spellingShingle |
Eicosapentaenoic acid (EPA) arctic diatom factorial design Salinity growth phase interactive effects microalgal biotechnology Steinrücken, Pia Mjøs, Svein Are Prestegard, Siv Kristin Erga, Svein Rune Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
topic_facet |
Eicosapentaenoic acid (EPA) arctic diatom factorial design Salinity growth phase interactive effects microalgal biotechnology |
description |
Microalgae with a high content of the omega-3 polyunsaturated fatty acids (PUFAs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are of great demand for microalgae-based technologies. An Arctic strain of the diatom Attheya septentrionalis was shown in previous experiments to increase its EPA content from 3.0 to 4.6% of dry weight (DW) in the nutrient-replete exponential phase and nutrient-depleted stationary phase, respectively. In the present study, a factorial-design experiment was used, to investigate this effect in more detail and in combination with varying salinities and irradiances. A mathematical model revealed that both growth phase and salinity, alone and in combination, influenced the EPA content significantly. Maximum EPA values of 7.1% DW were obtained at a salinity of 22 and after 5 days in stationary phase, and might be related to a decreased silica content, an accumulation of storage lipids containing EPA, or both. However, growth rates were lower for low salinity (0.54 and 0.57 d−1) than high salinity (0.77 and 0.98 d−1) cultures. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Steinrücken, Pia Mjøs, Svein Are Prestegard, Siv Kristin Erga, Svein Rune |
author_facet |
Steinrücken, Pia Mjøs, Svein Are Prestegard, Siv Kristin Erga, Svein Rune |
author_sort |
Steinrücken, Pia |
title |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_short |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_full |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_fullStr |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_full_unstemmed |
Enhancing EPA Content in an Arctic Diatom: A Factorial Design Study to Evaluate Interactive Effects of Growth Factors |
title_sort |
enhancing epa content in an arctic diatom: a factorial design study to evaluate interactive effects of growth factors |
publisher |
Frontiers |
publishDate |
2018 |
url |
https://hdl.handle.net/1956/17720 https://doi.org/10.3389/fpls.2018.00491 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
High-value fatty acids from microalgae. Bioprospecting and outdoor cultivation at northern latitudes urn:issn:1664-462X https://hdl.handle.net/1956/17720 https://doi.org/10.3389/fpls.2018.00491 cristin:1579768 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2018 The Author(s) |
op_doi |
https://doi.org/10.3389/fpls.2018.00491 |
container_title |
Frontiers in Plant Science |
container_volume |
9 |
_version_ |
1766322740959117312 |