Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies
Microalgae are considered to be an important and sustainable alternative to fish oil as a source for the polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Due to their health benefits, there is an increasing interest in the commercial application of thes...
Published in: | Algal Research |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2017
|
Subjects: | |
Online Access: | https://hdl.handle.net/1956/17719 https://doi.org/10.1016/j.algal.2017.07.030 |
id |
ftunivbergen:oai:bora.uib.no:1956/17719 |
---|---|
record_format |
openpolar |
spelling |
ftunivbergen:oai:bora.uib.no:1956/17719 2023-05-15T17:32:05+02:00 Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies Steinrücken, Pia Erga, Svein Rune Mjøs, Svein Are Kleivdal, Hans Torstein Prestegard, Siv Kristin 2017-09 application/pdf https://hdl.handle.net/1956/17719 https://doi.org/10.1016/j.algal.2017.07.030 eng eng Elsevier High-value fatty acids from microalgae. Bioprospecting and outdoor cultivation at northern latitudes urn:issn:2211-9264 https://hdl.handle.net/1956/17719 https://doi.org/10.1016/j.algal.2017.07.030 cristin:1519353 Attribution CC BY-NC-ND http://creativecommons.org/licenses/by-nc-nd/4.0/ Copyright 2017 The Author(s) 392 Algal Research 26 401- Bioprospecting Docosahexaenoic acid (DHA) Eicosapentaenoic acid (EPA) Microalgae Northern high latitudes Omega-3 fatty acids Peer reviewed Journal article 2017 ftunivbergen https://doi.org/10.1016/j.algal.2017.07.030 2023-03-14T17:43:21Z Microalgae are considered to be an important and sustainable alternative to fish oil as a source for the polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Due to their health benefits, there is an increasing interest in the commercial application of these fatty acids (FA) to health and dietary products, and to aquaculture feeds. However, FA from microalgae are still expensive to produce compared to fish or plant oils. With only a few microalgal strains being cultivated on a large scale for commercial PUFA production, prospecting for new, robust and fast-growing strains with increased PUFA content is essential in order to reduce production costs. Microalgae from northern high latitudes, exposed to cold temperatures, may be especially promising candidates as previous studies have shown increasing unsaturation of FA in response to decreasing growth temperatures in different microalgae, most likely to maintain membrane fluidity and function. We have designed a screening pipeline, targeting a focused search and selection for marine microalgal strains from extreme North Atlantic locations with high robustness and biomass production, and increased levels of EPA and DHA. The pipeline includes a rational sampling plan, isolation and cultivation of clonal strains, followed by a batch growth experiment designed to obtain information on robustness, growth characteristics, and the FA content of selected isolates during both nutrient replete exponential cultivation and nutrient limited stationary cultivation. A number of clonal cultures (N = 149) have been established, and twenty of these strains have been screened for growth and FA content and composition. Among those strains, three showed growth rates ≥ 0.7 d− 1 at temperatures of 15 °C or below, and high amounts of EPA (> 3% DW), suggesting their potential as candidates for large scale production. publishedVersion Article in Journal/Newspaper North Atlantic University of Bergen: Bergen Open Research Archive (BORA-UiB) Algal Research 26 392 401 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Bioprospecting Docosahexaenoic acid (DHA) Eicosapentaenoic acid (EPA) Microalgae Northern high latitudes Omega-3 fatty acids |
spellingShingle |
Bioprospecting Docosahexaenoic acid (DHA) Eicosapentaenoic acid (EPA) Microalgae Northern high latitudes Omega-3 fatty acids Steinrücken, Pia Erga, Svein Rune Mjøs, Svein Are Kleivdal, Hans Torstein Prestegard, Siv Kristin Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies |
topic_facet |
Bioprospecting Docosahexaenoic acid (DHA) Eicosapentaenoic acid (EPA) Microalgae Northern high latitudes Omega-3 fatty acids |
description |
Microalgae are considered to be an important and sustainable alternative to fish oil as a source for the polyunsaturated fatty acids (PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Due to their health benefits, there is an increasing interest in the commercial application of these fatty acids (FA) to health and dietary products, and to aquaculture feeds. However, FA from microalgae are still expensive to produce compared to fish or plant oils. With only a few microalgal strains being cultivated on a large scale for commercial PUFA production, prospecting for new, robust and fast-growing strains with increased PUFA content is essential in order to reduce production costs. Microalgae from northern high latitudes, exposed to cold temperatures, may be especially promising candidates as previous studies have shown increasing unsaturation of FA in response to decreasing growth temperatures in different microalgae, most likely to maintain membrane fluidity and function. We have designed a screening pipeline, targeting a focused search and selection for marine microalgal strains from extreme North Atlantic locations with high robustness and biomass production, and increased levels of EPA and DHA. The pipeline includes a rational sampling plan, isolation and cultivation of clonal strains, followed by a batch growth experiment designed to obtain information on robustness, growth characteristics, and the FA content of selected isolates during both nutrient replete exponential cultivation and nutrient limited stationary cultivation. A number of clonal cultures (N = 149) have been established, and twenty of these strains have been screened for growth and FA content and composition. Among those strains, three showed growth rates ≥ 0.7 d− 1 at temperatures of 15 °C or below, and high amounts of EPA (> 3% DW), suggesting their potential as candidates for large scale production. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Steinrücken, Pia Erga, Svein Rune Mjøs, Svein Are Kleivdal, Hans Torstein Prestegard, Siv Kristin |
author_facet |
Steinrücken, Pia Erga, Svein Rune Mjøs, Svein Are Kleivdal, Hans Torstein Prestegard, Siv Kristin |
author_sort |
Steinrücken, Pia |
title |
Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies |
title_short |
Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies |
title_full |
Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies |
title_fullStr |
Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies |
title_full_unstemmed |
Bioprospecting North Atlantic microalgae with fast growth and high polyunsaturated fatty acid (PUFA) content for microalgae-based technologies |
title_sort |
bioprospecting north atlantic microalgae with fast growth and high polyunsaturated fatty acid (pufa) content for microalgae-based technologies |
publisher |
Elsevier |
publishDate |
2017 |
url |
https://hdl.handle.net/1956/17719 https://doi.org/10.1016/j.algal.2017.07.030 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
392 Algal Research 26 401- |
op_relation |
High-value fatty acids from microalgae. Bioprospecting and outdoor cultivation at northern latitudes urn:issn:2211-9264 https://hdl.handle.net/1956/17719 https://doi.org/10.1016/j.algal.2017.07.030 cristin:1519353 |
op_rights |
Attribution CC BY-NC-ND http://creativecommons.org/licenses/by-nc-nd/4.0/ Copyright 2017 The Author(s) |
op_doi |
https://doi.org/10.1016/j.algal.2017.07.030 |
container_title |
Algal Research |
container_volume |
26 |
container_start_page |
392 |
op_container_end_page |
401 |
_version_ |
1766130036629307392 |