Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts
Prolonged production of Atlantic salmon (Salmo salar) post-smolts in closed-containment systems has prompted research into biological requirements under higher production intensities. This study examined the effect of fish density and specific water flow on skin health in post post-smolts particular...
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Format: | Article in Journal/Newspaper |
Language: | English |
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Online Access: | https://hdl.handle.net/1956/17352 https://doi.org/10.1016/j.aquaculture.2016.08.012 |
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ftunivbergen:oai:bora.uib.no:1956/17352 2023-05-15T15:32:13+02:00 Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts Sveen, Lene Rydal Timmerhaus, Gerrit Torgersen, Jacob Seilø Ytteborg, Elisabeth Jørgensen, Sven Martin Handeland, Sigurd Olav Stefansson, Sigurd Olav Nilsen, Tom Ole Calabrese, Sara Ebbesson, Lars O.E. Terjesen, Bendik Fyhn Takle, Harald Rune 2017-09-29T13:04:52Z application/pdf https://hdl.handle.net/1956/17352 https://doi.org/10.1016/j.aquaculture.2016.08.012 eng eng Elsevier Norges forskningsråd: 217502 Norges forskningsråd: 233870 Fiskeri- og havbruksnæringens forskningsfond: 900816 urn:issn:1873-5622 urn:issn:0044-8486 https://hdl.handle.net/1956/17352 https://doi.org/10.1016/j.aquaculture.2016.08.012 cristin:1389265 Attribution CC BY-NC-ND http://creativecommons.org/licenses/by-nc-nd/4.0/ Copyright 2016 Elsevier Aquaculture Closed-containment systems Skin health Fish density Specific water flow Fish welfare Peer reviewed Journal article 2017 ftunivbergen https://doi.org/10.1016/j.aquaculture.2016.08.012 2023-03-14T17:39:50Z Prolonged production of Atlantic salmon (Salmo salar) post-smolts in closed-containment systems has prompted research into biological requirements under higher production intensities. This study examined the effect of fish density and specific water flow on skin health in post post-smolts particularly focusing on epithelial cell morphology and gene expression. In the density experiment, post-smolts were kept at five different fish densities (25, 50, 75, 100 and 125 kg/m3) at a specific water flow rate of 0.6 l/kg/min. Microscopic examination of fluorescence stained whole-mount skin samples demonstrated differences in epithelial cell morphology with increased spacing between epithelial cells at 50 kg/m3 and 125 kg/m3. Gene expression analysis revealed increased transcription of mucin-like 2, cathepsins B, -D, -L, matrix metalloproteinase 9 and claudin 10 in fish reared at a density of 125 kg/m3, while only matrix metalloproteinase 9 and claudin 10 had increased transcription at a density of 100 kg/m3. Together, these results suggest structural alterations in the skin epithelium at densities ≥ 100 kg/m3. In the specific water flow experiment, four different water flow levels were established (0.2, 0.3, 0.4 and 0.5 l/kg/min) while the fish density was kept constant at 75 kg/m3. After eight weeks, transcription of mucin-like 2 and -5ac, inducible nitric oxide synthase, lysozyme and cathepsin B and -L increased in skin samples from fish reared in tanks with a specific water flow of ≤ 0.3 l/kg/min. Increased transcription of these genes implies activation of stress and immune responses in skin at low specific water flow. Results from this study suggests that skin is a sensitive organ for environmental changes, and suggests several molecular indicators that may be valuable in predicting the effects of varying rearing conditions on skin health. Further validation through long-term studies, combined with other health parameters is required for practical recommendations regarding critical fish density and water flow for ... Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) Aquaculture 464 629 637 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Closed-containment systems Skin health Fish density Specific water flow Fish welfare |
spellingShingle |
Closed-containment systems Skin health Fish density Specific water flow Fish welfare Sveen, Lene Rydal Timmerhaus, Gerrit Torgersen, Jacob Seilø Ytteborg, Elisabeth Jørgensen, Sven Martin Handeland, Sigurd Olav Stefansson, Sigurd Olav Nilsen, Tom Ole Calabrese, Sara Ebbesson, Lars O.E. Terjesen, Bendik Fyhn Takle, Harald Rune Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts |
topic_facet |
Closed-containment systems Skin health Fish density Specific water flow Fish welfare |
description |
Prolonged production of Atlantic salmon (Salmo salar) post-smolts in closed-containment systems has prompted research into biological requirements under higher production intensities. This study examined the effect of fish density and specific water flow on skin health in post post-smolts particularly focusing on epithelial cell morphology and gene expression. In the density experiment, post-smolts were kept at five different fish densities (25, 50, 75, 100 and 125 kg/m3) at a specific water flow rate of 0.6 l/kg/min. Microscopic examination of fluorescence stained whole-mount skin samples demonstrated differences in epithelial cell morphology with increased spacing between epithelial cells at 50 kg/m3 and 125 kg/m3. Gene expression analysis revealed increased transcription of mucin-like 2, cathepsins B, -D, -L, matrix metalloproteinase 9 and claudin 10 in fish reared at a density of 125 kg/m3, while only matrix metalloproteinase 9 and claudin 10 had increased transcription at a density of 100 kg/m3. Together, these results suggest structural alterations in the skin epithelium at densities ≥ 100 kg/m3. In the specific water flow experiment, four different water flow levels were established (0.2, 0.3, 0.4 and 0.5 l/kg/min) while the fish density was kept constant at 75 kg/m3. After eight weeks, transcription of mucin-like 2 and -5ac, inducible nitric oxide synthase, lysozyme and cathepsin B and -L increased in skin samples from fish reared in tanks with a specific water flow of ≤ 0.3 l/kg/min. Increased transcription of these genes implies activation of stress and immune responses in skin at low specific water flow. Results from this study suggests that skin is a sensitive organ for environmental changes, and suggests several molecular indicators that may be valuable in predicting the effects of varying rearing conditions on skin health. Further validation through long-term studies, combined with other health parameters is required for practical recommendations regarding critical fish density and water flow for ... |
format |
Article in Journal/Newspaper |
author |
Sveen, Lene Rydal Timmerhaus, Gerrit Torgersen, Jacob Seilø Ytteborg, Elisabeth Jørgensen, Sven Martin Handeland, Sigurd Olav Stefansson, Sigurd Olav Nilsen, Tom Ole Calabrese, Sara Ebbesson, Lars O.E. Terjesen, Bendik Fyhn Takle, Harald Rune |
author_facet |
Sveen, Lene Rydal Timmerhaus, Gerrit Torgersen, Jacob Seilø Ytteborg, Elisabeth Jørgensen, Sven Martin Handeland, Sigurd Olav Stefansson, Sigurd Olav Nilsen, Tom Ole Calabrese, Sara Ebbesson, Lars O.E. Terjesen, Bendik Fyhn Takle, Harald Rune |
author_sort |
Sveen, Lene Rydal |
title |
Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts |
title_short |
Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts |
title_full |
Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts |
title_fullStr |
Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts |
title_full_unstemmed |
Impact of fish density and specific water flow on skin properties in Atlantic salmon (Salmo salar L.) post-smolts |
title_sort |
impact of fish density and specific water flow on skin properties in atlantic salmon (salmo salar l.) post-smolts |
publisher |
Elsevier |
publishDate |
2017 |
url |
https://hdl.handle.net/1956/17352 https://doi.org/10.1016/j.aquaculture.2016.08.012 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Aquaculture |
op_relation |
Norges forskningsråd: 217502 Norges forskningsråd: 233870 Fiskeri- og havbruksnæringens forskningsfond: 900816 urn:issn:1873-5622 urn:issn:0044-8486 https://hdl.handle.net/1956/17352 https://doi.org/10.1016/j.aquaculture.2016.08.012 cristin:1389265 |
op_rights |
Attribution CC BY-NC-ND http://creativecommons.org/licenses/by-nc-nd/4.0/ Copyright 2016 Elsevier |
op_doi |
https://doi.org/10.1016/j.aquaculture.2016.08.012 |
container_title |
Aquaculture |
container_volume |
464 |
container_start_page |
629 |
op_container_end_page |
637 |
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1766362716295921664 |