Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species
Background: In the marine environment, where there are few absolute physical barriers, contemporary contact between previously isolated species can occur across great distances, and in some cases, may be inter-oceanic. An example of this can be seen in the minke whale species complex. Antarctic mink...
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Online Access: | https://hdl.handle.net/1956/17007 https://doi.org/10.1186/s12864-016-3416-5 |
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ftunivbergen:oai:bora.uib.no:1956/17007 2023-05-15T13:41:29+02:00 Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species Malde, Ketil Seliussen, Bjørghild Breistein Sanchez, Maria Quintela Dahle, Geir Besnier, Francois Skaug, Hans J. Øien, Nils Inge Solvang, Hiroko Kato Haug, Tore Skern-Mauritzen, Rasmus Kanda, Naohisa Pastene, Luis A. Jonassen, Inge Glover, Kevin 2017-11-07T13:58:38Z application/pdf https://hdl.handle.net/1956/17007 https://doi.org/10.1186/s12864-016-3416-5 eng eng BioMed Central urn:issn:1471-2164 https://hdl.handle.net/1956/17007 https://doi.org/10.1186/s12864-016-3416-5 cristin:1468529 Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2017 The Author(s) BMC Genomics Peer reviewed Journal article 2017 ftunivbergen https://doi.org/10.1186/s12864-016-3416-5 2023-03-14T17:41:39Z Background: In the marine environment, where there are few absolute physical barriers, contemporary contact between previously isolated species can occur across great distances, and in some cases, may be inter-oceanic. An example of this can be seen in the minke whale species complex. Antarctic minke whales are genetically and morphologically distinct from the common minke found in the north Atlantic and Pacific oceans, and the two species are estimated to have been isolated from each other for 5 million years or more. Recent atypical migrations from the southern to the northern hemisphere have been documented and fertile hybrids and back-crossed individuals between both species have also been identified. However, it is not known whether this represents a contemporary event, potentially driven by ecosystem changes in the Antarctic, or a sporadic occurrence happening over an evolutionary time-scale. We successfully used whole genome resequencing to identify a panel of diagnostic SNPs which now enable us address this evolutionary question. Results: A large number of SNPs displaying fixed or nearly fixed allele frequency differences among the minke whale species were identified from the sequence data. Five panels of putatively diagnostic markers were established on a genotyping platform for validation of allele frequencies; two panels (26 and 24 SNPs) separating the two species of minke whale, and three panels (22, 23, and 24 SNPs) differentiating the three subspecies of common minke whale. The panels were validated against a set of reference samples, demonstrating the ability to accurately identify back-crossed whales up to three generations. Conclusions: This work has resulted in the development of a panel of novel diagnostic genetic markers to address inter-oceanic and global contact among the genetically isolated minke whale species and sub-species. These markers, including a globally relevant genetic reference data set for this species complex, are now openly available for researchers interested in identifying ... Article in Journal/Newspaper Antarc* Antarctic minke whale North Atlantic University of Bergen: Bergen Open Research Archive (BORA-UiB) Antarctic Pacific The Antarctic BMC Genomics 18 1 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
Background: In the marine environment, where there are few absolute physical barriers, contemporary contact between previously isolated species can occur across great distances, and in some cases, may be inter-oceanic. An example of this can be seen in the minke whale species complex. Antarctic minke whales are genetically and morphologically distinct from the common minke found in the north Atlantic and Pacific oceans, and the two species are estimated to have been isolated from each other for 5 million years or more. Recent atypical migrations from the southern to the northern hemisphere have been documented and fertile hybrids and back-crossed individuals between both species have also been identified. However, it is not known whether this represents a contemporary event, potentially driven by ecosystem changes in the Antarctic, or a sporadic occurrence happening over an evolutionary time-scale. We successfully used whole genome resequencing to identify a panel of diagnostic SNPs which now enable us address this evolutionary question. Results: A large number of SNPs displaying fixed or nearly fixed allele frequency differences among the minke whale species were identified from the sequence data. Five panels of putatively diagnostic markers were established on a genotyping platform for validation of allele frequencies; two panels (26 and 24 SNPs) separating the two species of minke whale, and three panels (22, 23, and 24 SNPs) differentiating the three subspecies of common minke whale. The panels were validated against a set of reference samples, demonstrating the ability to accurately identify back-crossed whales up to three generations. Conclusions: This work has resulted in the development of a panel of novel diagnostic genetic markers to address inter-oceanic and global contact among the genetically isolated minke whale species and sub-species. These markers, including a globally relevant genetic reference data set for this species complex, are now openly available for researchers interested in identifying ... |
format |
Article in Journal/Newspaper |
author |
Malde, Ketil Seliussen, Bjørghild Breistein Sanchez, Maria Quintela Dahle, Geir Besnier, Francois Skaug, Hans J. Øien, Nils Inge Solvang, Hiroko Kato Haug, Tore Skern-Mauritzen, Rasmus Kanda, Naohisa Pastene, Luis A. Jonassen, Inge Glover, Kevin |
spellingShingle |
Malde, Ketil Seliussen, Bjørghild Breistein Sanchez, Maria Quintela Dahle, Geir Besnier, Francois Skaug, Hans J. Øien, Nils Inge Solvang, Hiroko Kato Haug, Tore Skern-Mauritzen, Rasmus Kanda, Naohisa Pastene, Luis A. Jonassen, Inge Glover, Kevin Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species |
author_facet |
Malde, Ketil Seliussen, Bjørghild Breistein Sanchez, Maria Quintela Dahle, Geir Besnier, Francois Skaug, Hans J. Øien, Nils Inge Solvang, Hiroko Kato Haug, Tore Skern-Mauritzen, Rasmus Kanda, Naohisa Pastene, Luis A. Jonassen, Inge Glover, Kevin |
author_sort |
Malde, Ketil |
title |
Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species |
title_short |
Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species |
title_full |
Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species |
title_fullStr |
Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species |
title_full_unstemmed |
Whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species |
title_sort |
whole genome resequencing reveals diagnostic markers for investigating global migration and hybridization between minke whale species |
publisher |
BioMed Central |
publishDate |
2017 |
url |
https://hdl.handle.net/1956/17007 https://doi.org/10.1186/s12864-016-3416-5 |
geographic |
Antarctic Pacific The Antarctic |
geographic_facet |
Antarctic Pacific The Antarctic |
genre |
Antarc* Antarctic minke whale North Atlantic |
genre_facet |
Antarc* Antarctic minke whale North Atlantic |
op_source |
BMC Genomics |
op_relation |
urn:issn:1471-2164 https://hdl.handle.net/1956/17007 https://doi.org/10.1186/s12864-016-3416-5 cristin:1468529 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0 Copyright 2017 The Author(s) |
op_doi |
https://doi.org/10.1186/s12864-016-3416-5 |
container_title |
BMC Genomics |
container_volume |
18 |
container_issue |
1 |
_version_ |
1766151432251113472 |