The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges
As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of t...
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Online Access: | https://hdl.handle.net/1956/15240 https://doi.org/10.3389/fmicb.2016.01781 |
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ftunivbergen:oai:bora.uib.no:1956/15240 2023-05-15T14:24:23+02:00 The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges Hestetun, Jon Thomassen Dahle, Håkon Jørgensen, Steffen Leth Olsen, Bernt Rydland Rapp, Hans Tore 2016-11-09 application/pdf https://hdl.handle.net/1956/15240 https://doi.org/10.3389/fmicb.2016.01781 eng eng Frontiers Carnivorous sponges of the Atlantic and Arctic Oceans. Phylogeny, taxonomy, distribution and microbial associations of the Cladorhizidae (Demospongiae, Poecilosclerida) urn:issn:1664-302X https://hdl.handle.net/1956/15240 https://doi.org/10.3389/fmicb.2016.01781 cristin:1394645 Attribution CC BY http://creativecommons.org/licenses/by/4.0/ Copyright 2016 Hestetun, Dahle, Jørgensen, Olsen and Rapp. 1781 Frontiers in Microbiology 7 Porifera Cladorhizidae Barbados Arctic mid-ocean ridge hydrothermal vent methane seep isotope Cladorhiza methanophila Peer reviewed Journal article 2016 ftunivbergen https://doi.org/10.3389/fmicb.2016.01781 2023-03-14T17:44:23Z As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of the methanotrophic carnivorous sponge Cladorhiza methanophila from a mud volcano-rich area at the Barbados Accretionary Prism, and five carnivorous species from the Jan Mayen Vent Field (JMVF) at the Arctic Mid-Ocean Ridge. Results from 16S rRNA microbiome data indicate the presence of a diverse assemblage of associated microorganisms in carnivorous sponges mainly from the Gamma- and Alphaproteobacteria, Flavobacteriaceae, and Thaumarchaeota. While the abundance of particular groups varied throughout the dataset, we found interesting similarities to previous microbiome results from non-carnivorous deep sea sponges, suggesting that the carnivorous sponges share characteristics of a previously hypothesized putative deep-sea sponge microbial community. Chemolithoautotrophic symbiosis was confirmed for C. methanophila through a microbial community with a high abundance of Methylococcales and very light isotopic δ13C and δ15N ratios (-60 to -66‰/3.5 to 5.2‰) compared to the other cladorhizid species (-22 to -24‰/8.5 to 10.5‰). We provide evidence for the presence of putative sulfur-oxidizing Gammaproteobacteria in the arctic cladorhizids; however, δ13C and δ15N signatures did not provide evidence for significant chemoautotrophic symbiosis in this case, and the slightly higher abundance of cladorhizids at the JMVF site compared to the nearby deep sea likely stem from an increased abundance of prey rather than a more direct vent association. The phylogenetic position of C. methanophila in relation to other carnivorous sponges was established using a three-gene phylogenetic analysis, and it was found to be closely related to other non-methanotrophic Cladorhiza species with a similar morphology included in the dataset, ... Article in Journal/Newspaper Arctic Arctic Jan Mayen University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic Jan Mayen Frontiers in Microbiology 7 |
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Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
Porifera Cladorhizidae Barbados Arctic mid-ocean ridge hydrothermal vent methane seep isotope Cladorhiza methanophila |
spellingShingle |
Porifera Cladorhizidae Barbados Arctic mid-ocean ridge hydrothermal vent methane seep isotope Cladorhiza methanophila Hestetun, Jon Thomassen Dahle, Håkon Jørgensen, Steffen Leth Olsen, Bernt Rydland Rapp, Hans Tore The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
topic_facet |
Porifera Cladorhizidae Barbados Arctic mid-ocean ridge hydrothermal vent methane seep isotope Cladorhiza methanophila |
description |
As shown by recent studies, filter-feeding sponges are known to host a wide variety of microorganisms. However, the microbial community of the non-filtering carnivorous sponges (Porifera, Cladorhizidae) has been the subject of less scrutiny. Here, we present the results from a comparative study of the methanotrophic carnivorous sponge Cladorhiza methanophila from a mud volcano-rich area at the Barbados Accretionary Prism, and five carnivorous species from the Jan Mayen Vent Field (JMVF) at the Arctic Mid-Ocean Ridge. Results from 16S rRNA microbiome data indicate the presence of a diverse assemblage of associated microorganisms in carnivorous sponges mainly from the Gamma- and Alphaproteobacteria, Flavobacteriaceae, and Thaumarchaeota. While the abundance of particular groups varied throughout the dataset, we found interesting similarities to previous microbiome results from non-carnivorous deep sea sponges, suggesting that the carnivorous sponges share characteristics of a previously hypothesized putative deep-sea sponge microbial community. Chemolithoautotrophic symbiosis was confirmed for C. methanophila through a microbial community with a high abundance of Methylococcales and very light isotopic δ13C and δ15N ratios (-60 to -66‰/3.5 to 5.2‰) compared to the other cladorhizid species (-22 to -24‰/8.5 to 10.5‰). We provide evidence for the presence of putative sulfur-oxidizing Gammaproteobacteria in the arctic cladorhizids; however, δ13C and δ15N signatures did not provide evidence for significant chemoautotrophic symbiosis in this case, and the slightly higher abundance of cladorhizids at the JMVF site compared to the nearby deep sea likely stem from an increased abundance of prey rather than a more direct vent association. The phylogenetic position of C. methanophila in relation to other carnivorous sponges was established using a three-gene phylogenetic analysis, and it was found to be closely related to other non-methanotrophic Cladorhiza species with a similar morphology included in the dataset, ... |
format |
Article in Journal/Newspaper |
author |
Hestetun, Jon Thomassen Dahle, Håkon Jørgensen, Steffen Leth Olsen, Bernt Rydland Rapp, Hans Tore |
author_facet |
Hestetun, Jon Thomassen Dahle, Håkon Jørgensen, Steffen Leth Olsen, Bernt Rydland Rapp, Hans Tore |
author_sort |
Hestetun, Jon Thomassen |
title |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_short |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_full |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_fullStr |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_full_unstemmed |
The Microbiome and Occurrence of Methanotrophy in Carnivorous Sponges |
title_sort |
microbiome and occurrence of methanotrophy in carnivorous sponges |
publisher |
Frontiers |
publishDate |
2016 |
url |
https://hdl.handle.net/1956/15240 https://doi.org/10.3389/fmicb.2016.01781 |
geographic |
Arctic Jan Mayen |
geographic_facet |
Arctic Jan Mayen |
genre |
Arctic Arctic Jan Mayen |
genre_facet |
Arctic Arctic Jan Mayen |
op_source |
1781 Frontiers in Microbiology 7 |
op_relation |
Carnivorous sponges of the Atlantic and Arctic Oceans. Phylogeny, taxonomy, distribution and microbial associations of the Cladorhizidae (Demospongiae, Poecilosclerida) urn:issn:1664-302X https://hdl.handle.net/1956/15240 https://doi.org/10.3389/fmicb.2016.01781 cristin:1394645 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0/ Copyright 2016 Hestetun, Dahle, Jørgensen, Olsen and Rapp. |
op_doi |
https://doi.org/10.3389/fmicb.2016.01781 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
_version_ |
1766296801192706048 |