Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism
Background: Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver prote...
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ftunivbergen:oai:bora.uib.no:1956/12520 2023-05-15T15:27:23+02:00 Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism Yadetie, Fekadu Bjørneklett, Silje Garberg, Hilde K Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd André 2016-08-05T16:03:09Z application/pdf application/octet-stream https://hdl.handle.net/1956/12520 https://doi.org/10.1186/s12864-016-2864-2 eng eng BioMed Central Ltd. https://hdl.handle.net/1956/12520 https://doi.org/10.1186/s12864-016-2864-2 This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. http://creativecommons.org/licenses/by/4.0/ The Author(s). VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Peer reviewed Journal article 2016 ftunivbergen https://doi.org/10.1186/s12864-016-2864-2 2023-03-14T17:41:43Z Background: Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Results: Out of a toltal of 1143 proteins quantified, 125 proteins were differentially regulated between MeHg-treated samples and controls. Using various bioinformatics tools, we performed gene ontology, pathway and network enrichment analysis, which indicated that proteins and pathways mainly related to energy metabolism, antioxidant defense, cytoskeleton remodeling, and protein synthesis were regulated in the hepatic proteome after MeHg exposure. Comparison with previous gene expression data strengthened these results, and further supported that MeHg predominantly affects many energy metabolism pathways, presumably through its strong induction of oxidative stress. Some enzymes known to have functionally important oxidation-sensitive cysteine residues in other animals are among the differentially regulated proteins, suggesting their modulations by MeHg-induced oxidative stress. Integrated analysis of the proteomics dataset combined with previous gene expression dataset showed a more pronounced effect of MeHg on amino acid, glucose and fatty acid metabolic pathways, and suggested possible interactions of the cellular energy metabolism and antioxidant defense pathways. Conclusions: MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver. The energy pathways appear to be modulated through MeHg-induced oxidative stress, possibly mediated by oxidation sensitive enzymes. publishedVersion Article in Journal/Newspaper atlantic cod Gadus morhua University of Bergen: Bergen Open Research Archive (BORA-UiB) BMC Genomics 17 1 |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
language |
English |
topic |
VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
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VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 Yadetie, Fekadu Bjørneklett, Silje Garberg, Hilde K Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd André Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
topic_facet |
VDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470 |
description |
Background: Methylmecury (MeHg) is a widely distributed environmental pollutant with considerable risk to both human health and wildlife. To gain better insight into the underlying mechanisms of MeHg-mediated toxicity, we have used label-free quantitative mass spectrometry to analyze the liver proteome of Atlantic cod (Gadus morhua) exposed in vivo to MeHg (0, 0.5, 2 mg/kg body weight) for 2 weeks. Results: Out of a toltal of 1143 proteins quantified, 125 proteins were differentially regulated between MeHg-treated samples and controls. Using various bioinformatics tools, we performed gene ontology, pathway and network enrichment analysis, which indicated that proteins and pathways mainly related to energy metabolism, antioxidant defense, cytoskeleton remodeling, and protein synthesis were regulated in the hepatic proteome after MeHg exposure. Comparison with previous gene expression data strengthened these results, and further supported that MeHg predominantly affects many energy metabolism pathways, presumably through its strong induction of oxidative stress. Some enzymes known to have functionally important oxidation-sensitive cysteine residues in other animals are among the differentially regulated proteins, suggesting their modulations by MeHg-induced oxidative stress. Integrated analysis of the proteomics dataset combined with previous gene expression dataset showed a more pronounced effect of MeHg on amino acid, glucose and fatty acid metabolic pathways, and suggested possible interactions of the cellular energy metabolism and antioxidant defense pathways. Conclusions: MeHg disrupts mainly redox homeostasis and energy generating metabolic pathways in cod liver. The energy pathways appear to be modulated through MeHg-induced oxidative stress, possibly mediated by oxidation sensitive enzymes. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Yadetie, Fekadu Bjørneklett, Silje Garberg, Hilde K Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd André |
author_facet |
Yadetie, Fekadu Bjørneklett, Silje Garberg, Hilde K Oveland, Eystein Berven, Frode Goksøyr, Anders Karlsen, Odd André |
author_sort |
Yadetie, Fekadu |
title |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_short |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_full |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_fullStr |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_full_unstemmed |
Quantitative analyses of the hepatic proteome of methylmercury-exposed Atlantic cod (Gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
title_sort |
quantitative analyses of the hepatic proteome of methylmercury-exposed atlantic cod (gadus morhua) suggest oxidative stress-mediated effects on cellular energy metabolism |
publisher |
BioMed Central Ltd. |
publishDate |
2016 |
url |
https://hdl.handle.net/1956/12520 https://doi.org/10.1186/s12864-016-2864-2 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://hdl.handle.net/1956/12520 https://doi.org/10.1186/s12864-016-2864-2 |
op_rights |
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. http://creativecommons.org/licenses/by/4.0/ The Author(s). |
op_doi |
https://doi.org/10.1186/s12864-016-2864-2 |
container_title |
BMC Genomics |
container_volume |
17 |
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1 |
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1766357825593802752 |