The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts
Water homeostasis and the structural integrity of the vertebrate lens is partially mediated by AQP0 channels. Emerging evidence indicates that external pH may be involved in channel gating. Here we show that a tetraploid teleost, the Atlantic salmon, retains 4 aqp0 genes (aqp0a1, -0a2, -0b1, and -0b...
Published in: | The FASEB Journal |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Federation of American Society of Experimental Biology
2015
|
Subjects: | |
Online Access: | http://hdl.handle.net/1956/11046 https://doi.org/10.1096/fj.14-267625 |
id |
ftunivbergen:oai:bora.uib.no:1956/11046 |
---|---|
record_format |
openpolar |
spelling |
ftunivbergen:oai:bora.uib.no:1956/11046 2023-05-15T15:32:44+02:00 The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts Chauvigné, Francois Zapater, Cinta Stavang, Jon Anders Taranger, Geir Lasse Cerdà, Joan Finn, Roderick Nigel 2015-12-21T20:13:49Z application/pdf http://hdl.handle.net/1956/11046 https://doi.org/10.1096/fj.14-267625 eng eng Federation of American Society of Experimental Biology urn:issn:0892-6638 http://hdl.handle.net/1956/11046 https://doi.org/10.1096/fj.14-267625 cristin:1263517 Attribution CC BY http://creativecommons.org/licenses/by/4.0/ Copyright 2015 The Authors aquaporin water transport permeability lens cataract VDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Marine biology: 497 Peer reviewed Journal article 2015 ftunivbergen https://doi.org/10.1096/fj.14-267625 2023-03-14T17:39:54Z Water homeostasis and the structural integrity of the vertebrate lens is partially mediated by AQP0 channels. Emerging evidence indicates that external pH may be involved in channel gating. Here we show that a tetraploid teleost, the Atlantic salmon, retains 4 aqp0 genes (aqp0a1, -0a2, -0b1, and -0b2), which are highly, but not exclusively, expressed in the lens. Functional characterization reveals that, although each paralog permeates water efficiently, the permeability is respectively shifted to the neutral, alkaline, or acidic pH in Aqp0a1, -0a2, and -0b1, whereas that of Aqp0b2 is not regulated by external pH. Mutagenesis studies demonstrate that Ser38, His39, and His40 residues in the extracellular transmembrane domain of α-helix 2 facing the water pore are critical for the pH modulation of water transport. To validate these findings, we show that both zebrafish Aqp0a and -0b are functional water channels with respective pH sensitivities toward alkaline or acid pH ranges and that an N-terminal allelic variant (Ser19) of Aqp0b exists that abolishes water transport in Xenopus laevis oocytes. The data suggest that the alkaline pH sensitivity is a conserved trait in teleost Aqp0 a-type channels, whereas mammalian AQP0 and some teleost Aqp0 b-type channels display an acidic pH permeation preference.—Chauvigné, F., Zapater, C., Stavang, J. A., Taranger, G. L., Cerdà, J., Finn, R. N. The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts. publishedVersion Article in Journal/Newspaper Atlantic salmon University of Bergen: Bergen Open Research Archive (BORA-UiB) Finn ENVELOPE(12.739,12.739,65.935,65.935) The FASEB Journal 29 5 2172 2184 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
aquaporin water transport permeability lens cataract VDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Marine biology: 497 |
spellingShingle |
aquaporin water transport permeability lens cataract VDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Marine biology: 497 Chauvigné, Francois Zapater, Cinta Stavang, Jon Anders Taranger, Geir Lasse Cerdà, Joan Finn, Roderick Nigel The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts |
topic_facet |
aquaporin water transport permeability lens cataract VDP::Matematikk og naturvitenskap: 400::Zoologiske og botaniske fag: 480::Marinbiologi: 497 VDP::Mathematics and natural scienses: 400::Zoology and botany: 480::Marine biology: 497 |
description |
Water homeostasis and the structural integrity of the vertebrate lens is partially mediated by AQP0 channels. Emerging evidence indicates that external pH may be involved in channel gating. Here we show that a tetraploid teleost, the Atlantic salmon, retains 4 aqp0 genes (aqp0a1, -0a2, -0b1, and -0b2), which are highly, but not exclusively, expressed in the lens. Functional characterization reveals that, although each paralog permeates water efficiently, the permeability is respectively shifted to the neutral, alkaline, or acidic pH in Aqp0a1, -0a2, and -0b1, whereas that of Aqp0b2 is not regulated by external pH. Mutagenesis studies demonstrate that Ser38, His39, and His40 residues in the extracellular transmembrane domain of α-helix 2 facing the water pore are critical for the pH modulation of water transport. To validate these findings, we show that both zebrafish Aqp0a and -0b are functional water channels with respective pH sensitivities toward alkaline or acid pH ranges and that an N-terminal allelic variant (Ser19) of Aqp0b exists that abolishes water transport in Xenopus laevis oocytes. The data suggest that the alkaline pH sensitivity is a conserved trait in teleost Aqp0 a-type channels, whereas mammalian AQP0 and some teleost Aqp0 b-type channels display an acidic pH permeation preference.—Chauvigné, F., Zapater, C., Stavang, J. A., Taranger, G. L., Cerdà, J., Finn, R. N. The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Chauvigné, Francois Zapater, Cinta Stavang, Jon Anders Taranger, Geir Lasse Cerdà, Joan Finn, Roderick Nigel |
author_facet |
Chauvigné, Francois Zapater, Cinta Stavang, Jon Anders Taranger, Geir Lasse Cerdà, Joan Finn, Roderick Nigel |
author_sort |
Chauvigné, Francois |
title |
The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts |
title_short |
The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts |
title_full |
The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts |
title_fullStr |
The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts |
title_full_unstemmed |
The pH sensitivity of Aqp0 channels in tetraploid and diploid teleosts |
title_sort |
ph sensitivity of aqp0 channels in tetraploid and diploid teleosts |
publisher |
Federation of American Society of Experimental Biology |
publishDate |
2015 |
url |
http://hdl.handle.net/1956/11046 https://doi.org/10.1096/fj.14-267625 |
long_lat |
ENVELOPE(12.739,12.739,65.935,65.935) |
geographic |
Finn |
geographic_facet |
Finn |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
urn:issn:0892-6638 http://hdl.handle.net/1956/11046 https://doi.org/10.1096/fj.14-267625 cristin:1263517 |
op_rights |
Attribution CC BY http://creativecommons.org/licenses/by/4.0/ Copyright 2015 The Authors |
op_doi |
https://doi.org/10.1096/fj.14-267625 |
container_title |
The FASEB Journal |
container_volume |
29 |
container_issue |
5 |
container_start_page |
2172 |
op_container_end_page |
2184 |
_version_ |
1766363224069898240 |