Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard
Extreme changes in light and cold water temperatures throughout the annual cycle in the Arctic Ocean create a unique habitat that selects for particular microorganisms - including marine viruses. This study investigated diversity of ecologically significant viral groups at two marine sampling statio...
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The University of Bergen
2015
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ftunivbergen:oai:bora.uib.no:1956/10976 2023-05-15T14:37:40+02:00 Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard Olesin, Emily Maria 2015-10-22 5104976 bytes application/pdf http://hdl.handle.net/1956/10976 eng eng The University of Bergen http://hdl.handle.net/1956/10976 Copyright the Author. All rights reserved virus Arctic sequencing g23 phoH MCP 751999 Master thesis 2015 ftunivbergen 2023-03-14T17:39:36Z Extreme changes in light and cold water temperatures throughout the annual cycle in the Arctic Ocean create a unique habitat that selects for particular microorganisms - including marine viruses. This study investigated diversity of ecologically significant viral groups at two marine sampling stations during the dark period in the Arctic Ocean north of the Svalbard archipelago through pyrosequencing of signature genes. Sequence data for three viral signature genes (g23, phoH, and MCP) were examined within the context of physical and biological environmental parameters to characterize the viral communities within several Arctic Ocean water masses of differing origin. Genotypic fingerprinting information from previous T4- like virus diversity investigations was used to explore phylogenetic relationships between Arctic Ocean g23 genotypes examined in this thesis to a global diversity of T4-like viruses isolated from various environments. Our findings show that marine viral communities exhibit dominant and rare types that vary proportionally in abundance between water masses, and that the available prokaryotic host communities vary similarly. The biogeographic examination showed that many of the dominant Arctic Ocean T4-like genotypes from this study are possibly endemic to the arctic, while others show similarity to globally distributed types, supporting the paradigm that local viral diversity may be high while also being low globally. Additionally, this study compared sequenced datasets of g23 amplicons from the same water samples generated using three widely- implemented sequencing platforms (Roche/454, Illumina, and Ion Torrent) in order to assess comparability of data from newer platforms for viral diversity investigations to pyrosequencing data. The platform comparison revealed that clustering of signature gene sequences into OTUs based on 90% similarity resulted in preservation of broad patterns in between-sample diversity, and also that sequence read data generated using Illumina appear most similar to ... Master Thesis Arctic Arctic Ocean Svalbard University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic Arctic Ocean Svalbard Svalbard Archipelago |
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Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
topic |
virus Arctic sequencing g23 phoH MCP 751999 |
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virus Arctic sequencing g23 phoH MCP 751999 Olesin, Emily Maria Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard |
topic_facet |
virus Arctic sequencing g23 phoH MCP 751999 |
description |
Extreme changes in light and cold water temperatures throughout the annual cycle in the Arctic Ocean create a unique habitat that selects for particular microorganisms - including marine viruses. This study investigated diversity of ecologically significant viral groups at two marine sampling stations during the dark period in the Arctic Ocean north of the Svalbard archipelago through pyrosequencing of signature genes. Sequence data for three viral signature genes (g23, phoH, and MCP) were examined within the context of physical and biological environmental parameters to characterize the viral communities within several Arctic Ocean water masses of differing origin. Genotypic fingerprinting information from previous T4- like virus diversity investigations was used to explore phylogenetic relationships between Arctic Ocean g23 genotypes examined in this thesis to a global diversity of T4-like viruses isolated from various environments. Our findings show that marine viral communities exhibit dominant and rare types that vary proportionally in abundance between water masses, and that the available prokaryotic host communities vary similarly. The biogeographic examination showed that many of the dominant Arctic Ocean T4-like genotypes from this study are possibly endemic to the arctic, while others show similarity to globally distributed types, supporting the paradigm that local viral diversity may be high while also being low globally. Additionally, this study compared sequenced datasets of g23 amplicons from the same water samples generated using three widely- implemented sequencing platforms (Roche/454, Illumina, and Ion Torrent) in order to assess comparability of data from newer platforms for viral diversity investigations to pyrosequencing data. The platform comparison revealed that clustering of signature gene sequences into OTUs based on 90% similarity resulted in preservation of broad patterns in between-sample diversity, and also that sequence read data generated using Illumina appear most similar to ... |
format |
Master Thesis |
author |
Olesin, Emily Maria |
author_facet |
Olesin, Emily Maria |
author_sort |
Olesin, Emily Maria |
title |
Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard |
title_short |
Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard |
title_full |
Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard |
title_fullStr |
Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard |
title_full_unstemmed |
Diversity of dsDNA marine viral groups during winter in the Arctic Ocean north of Svalbard |
title_sort |
diversity of dsdna marine viral groups during winter in the arctic ocean north of svalbard |
publisher |
The University of Bergen |
publishDate |
2015 |
url |
http://hdl.handle.net/1956/10976 |
geographic |
Arctic Arctic Ocean Svalbard Svalbard Archipelago |
geographic_facet |
Arctic Arctic Ocean Svalbard Svalbard Archipelago |
genre |
Arctic Arctic Ocean Svalbard |
genre_facet |
Arctic Arctic Ocean Svalbard |
op_relation |
http://hdl.handle.net/1956/10976 |
op_rights |
Copyright the Author. All rights reserved |
_version_ |
1766309883741732864 |