Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability
Although cold environments are major contributors to global biogeochemical cycles, comparatively little is known about their microbial community function, structure, and limits of activity. In this study a microcosm based approach was used to investigate the effects of temperature, and methanogenic...
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ftunivbergen:oai:bora.uib.no:1956/10084 2023-05-15T15:00:30+02:00 Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil 2015-06-26T11:18:16Z application/pdf http://hdl.handle.net/1956/10084 https://doi.org/10.1371/journal.pone.0129733 eng eng PLoS urn:issn:1932-6203 http://hdl.handle.net/1956/10084 https://doi.org/10.1371/journal.pone.0129733 cristin:1250824 Copyright 2015 Blake et al. http://creativecommons.org/licenses/by/4.0/ e0129733 PLoS ONE 10 6 Peer reviewed Journal article 2015 ftunivbergen https://doi.org/10.1371/journal.pone.0129733 2023-03-14T17:44:40Z Although cold environments are major contributors to global biogeochemical cycles, comparatively little is known about their microbial community function, structure, and limits of activity. In this study a microcosm based approach was used to investigate the effects of temperature, and methanogenic substrate amendment, (acetate, methanol and H2/CO2) on methanogen activity and methanogen community structure in high Arctic wetlands (Solvatnet and Stuphallet, Svalbard). Methane production was not detected in Stuphallet sediment microcosms (over a 150 day period) and occurred within Solvatnet sediments microcosms (within 24 hours) at temperatures from 5 to 40°C, the maximum temperature being at far higher than in situ maximum temperatures (which range from air temperatures of -1.4 to 14.1°C during summer months). Distinct responses were observed in the Solvatnet methanogen community under different short term incubation conditions. Specifically, different communities were selected at higher and lower temperatures. At lower temperatures (5°C) addition of exogenous substrates (acetate, methanol or H2/CO2) had no stimulatory effect on the rate of methanogenesis or on methanogen community structure. The community in these incubations was dominated by members of the Methanoregulaceae/WCHA2-08 family-level group, which were most similar to the psychrotolerant hydrogenotrophic methanogen Methanosphaerula palustris strain E1-9c. In contrast, at higher temperatures, substrate amendment enhanced methane production in H2/CO2 amended microcosms, and played a clear role in structuring methanogen communities. Specifically, at 30°C members of the Methanoregulaceae/WCHA2-08 predominated following incubation with H2/CO2, and Methanosarcinaceaeand Methanosaetaceae were enriched in response to acetate addition. These results may indicate that in transiently cold environments, methanogen communities can rapidly respond to moderate short term increases in temperature, but not necessarily to the seasonal release of previously frozen ... Article in Journal/Newspaper Arctic Svalbard University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic Svalbard PLOS ONE 10 6 e0129733 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
Although cold environments are major contributors to global biogeochemical cycles, comparatively little is known about their microbial community function, structure, and limits of activity. In this study a microcosm based approach was used to investigate the effects of temperature, and methanogenic substrate amendment, (acetate, methanol and H2/CO2) on methanogen activity and methanogen community structure in high Arctic wetlands (Solvatnet and Stuphallet, Svalbard). Methane production was not detected in Stuphallet sediment microcosms (over a 150 day period) and occurred within Solvatnet sediments microcosms (within 24 hours) at temperatures from 5 to 40°C, the maximum temperature being at far higher than in situ maximum temperatures (which range from air temperatures of -1.4 to 14.1°C during summer months). Distinct responses were observed in the Solvatnet methanogen community under different short term incubation conditions. Specifically, different communities were selected at higher and lower temperatures. At lower temperatures (5°C) addition of exogenous substrates (acetate, methanol or H2/CO2) had no stimulatory effect on the rate of methanogenesis or on methanogen community structure. The community in these incubations was dominated by members of the Methanoregulaceae/WCHA2-08 family-level group, which were most similar to the psychrotolerant hydrogenotrophic methanogen Methanosphaerula palustris strain E1-9c. In contrast, at higher temperatures, substrate amendment enhanced methane production in H2/CO2 amended microcosms, and played a clear role in structuring methanogen communities. Specifically, at 30°C members of the Methanoregulaceae/WCHA2-08 predominated following incubation with H2/CO2, and Methanosarcinaceaeand Methanosaetaceae were enriched in response to acetate addition. These results may indicate that in transiently cold environments, methanogen communities can rapidly respond to moderate short term increases in temperature, but not necessarily to the seasonal release of previously frozen ... |
format |
Article in Journal/Newspaper |
author |
Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil |
spellingShingle |
Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
author_facet |
Blake, Lynsay Tveit, Alexander Tøsdal Øvreås, Lise Head, Ian M. Gray, Neil |
author_sort |
Blake, Lynsay |
title |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_short |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_full |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_fullStr |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_full_unstemmed |
Response of Methanogens in Arctic Sediments to Temperature and Methanogenic Substrate Availability |
title_sort |
response of methanogens in arctic sediments to temperature and methanogenic substrate availability |
publisher |
PLoS |
publishDate |
2015 |
url |
http://hdl.handle.net/1956/10084 https://doi.org/10.1371/journal.pone.0129733 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Svalbard |
genre_facet |
Arctic Svalbard |
op_source |
e0129733 PLoS ONE 10 6 |
op_relation |
urn:issn:1932-6203 http://hdl.handle.net/1956/10084 https://doi.org/10.1371/journal.pone.0129733 cristin:1250824 |
op_rights |
Copyright 2015 Blake et al. http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1371/journal.pone.0129733 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
6 |
container_start_page |
e0129733 |
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1766332599049912320 |