Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-l...
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Online Access: | https://hdl.handle.net/11250/3112081 https://doi.org/10.3390/molecules28124728 |
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ftunivbergen:oai:bora.uib.no:11250/3112081 2024-02-11T10:02:05+01:00 Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth 2023 application/pdf https://hdl.handle.net/11250/3112081 https://doi.org/10.3390/molecules28124728 eng eng MDPI urn:issn:1431-5157 https://hdl.handle.net/11250/3112081 https://doi.org/10.3390/molecules28124728 cristin:2171072 Molecules. 2023, 28 (12), 4728. Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2023 The Author(s) 4728 Molecules 28 12 Journal article Peer reviewed 2023 ftunivbergen https://doi.org/10.3390/molecules28124728 2024-01-25T00:08:37Z A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL−1), limits of detection and quantification (0.05 and 0.1 ng mL−1, respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. publishedVersion Article in Journal/Newspaper Atlantic salmon University of Bergen: Bergen Open Research Archive (BORA-UiB) Molecules 28 12 4728 |
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Open Polar |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
language |
English |
description |
A simple and rapid method for the extraction of D-series resolvins (RvD1, RvD2, RvD3, RvD4, RvD5) released into Leibovitz’s L-15 complete medium by head kidney cells from Atlantic salmon and the further determination of liquid chromatography triple quadrupole mass spectrometry is proposed. A three-level factorial design was proposed to select the optimal concentrations of internal standards that were used in the evaluation of the performance parameters, such as linear range (0.1–50 ng mL−1), limits of detection and quantification (0.05 and 0.1 ng mL−1, respectively), and recovery values ranging from 96.9 to 99.8%. The optimized method was used to determine the stimulated production of resolvins by head kidney cells exposed to docosahexaenoic acid, and the results indicated that it is possible that the production was controlled by circadian responses. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth |
spellingShingle |
Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
author_facet |
Araujo, Pedro Iqbal, Sarah Arnø, Aleksander Espe, Marit Holen, Elisabeth |
author_sort |
Araujo, Pedro |
title |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_short |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_full |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_fullStr |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_full_unstemmed |
Validation of a Liquid–Liquid Extraction Method to Study the Temporal Production of D-Series Resolvins by Head Kidney Cells from Atlantic Salmon (Salmon salar) Exposed to Docosahexaenoic Acid |
title_sort |
validation of a liquid–liquid extraction method to study the temporal production of d-series resolvins by head kidney cells from atlantic salmon (salmon salar) exposed to docosahexaenoic acid |
publisher |
MDPI |
publishDate |
2023 |
url |
https://hdl.handle.net/11250/3112081 https://doi.org/10.3390/molecules28124728 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
4728 Molecules 28 12 |
op_relation |
urn:issn:1431-5157 https://hdl.handle.net/11250/3112081 https://doi.org/10.3390/molecules28124728 cristin:2171072 Molecules. 2023, 28 (12), 4728. |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2023 The Author(s) |
op_doi |
https://doi.org/10.3390/molecules28124728 |
container_title |
Molecules |
container_volume |
28 |
container_issue |
12 |
container_start_page |
4728 |
_version_ |
1790597986658549760 |