MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming
A Multi-Locus Variable number of tandem repeat Analysis (MLVA) genotyping scheme was developed for the epidemiological study of Moritella viscosa, which causes ‘winter ulcer’ predominantly in sea-reared Atlantic salmon (Salmo salar L.). The assay involves multiplex PCR amplification of six Variable...
Published in: | Journal of Fish Diseases |
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Language: | English |
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Online Access: | https://hdl.handle.net/11250/3083300 https://doi.org/10.1111/jfd.13766 |
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ftunivbergen:oai:bora.uib.no:11250/3083300 2023-09-05T13:18:13+02:00 MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming Sørgaard, Martin Sveinsson, Karoline Overn Patel, Sonal Nilsen, Hanne Katrine Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan John Gulla, Snorre 2023 application/pdf https://hdl.handle.net/11250/3083300 https://doi.org/10.1111/jfd.13766 eng eng Wiley urn:issn:0140-7775 https://hdl.handle.net/11250/3083300 https://doi.org/10.1111/jfd.13766 cristin:2145160 Journal of Fish Diseases. 2023, 46 (5), 535-543. Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2023 The Authors. Journal of Fish Diseases 535-543 46 5 Journal article Peer reviewed 2023 ftunivbergen https://doi.org/10.1111/jfd.13766 2023-08-16T23:07:22Z A Multi-Locus Variable number of tandem repeat Analysis (MLVA) genotyping scheme was developed for the epidemiological study of Moritella viscosa, which causes ‘winter ulcer’ predominantly in sea-reared Atlantic salmon (Salmo salar L.). The assay involves multiplex PCR amplification of six Variable Number of Tandem Repeat (VNTR) loci, followed by capillary electrophoresis and data interpretation. A collection of 747 spatiotemporally diverse M. viscosa isolates from nine fish species was analysed, the majority from farmed Norwegian salmon. MLVA distributed 76% of the isolates across three major clonal complexes (CC1, CC2 and CC3), with the remaining forming minor clusters and singletons. While 90% of the salmon isolates belong to either CC1, CC2 or CC3, only 20% of the isolates recovered from other fish species do so, indicating a considerable degree of host specificity. We further highlight a series of ‘clonal shifts’ amongst Norwegian salmon isolates over the 35-year sampling period, with CC1 showing exclusive predominance prior to the emergence of CC2, which was later supplanted by CC3, before the recent re-emergence of CC1. Apparently, these shifts have rapidly swept the entire Norwegian coastline and conceivably, as suggested by typing of a small number of non-Norwegian isolates, the Northeast Atlantic region as a whole. publishedVersion Article in Journal/Newspaper Atlantic salmon Northeast Atlantic Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) Journal of Fish Diseases 46 5 535 543 |
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Open Polar |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
language |
English |
description |
A Multi-Locus Variable number of tandem repeat Analysis (MLVA) genotyping scheme was developed for the epidemiological study of Moritella viscosa, which causes ‘winter ulcer’ predominantly in sea-reared Atlantic salmon (Salmo salar L.). The assay involves multiplex PCR amplification of six Variable Number of Tandem Repeat (VNTR) loci, followed by capillary electrophoresis and data interpretation. A collection of 747 spatiotemporally diverse M. viscosa isolates from nine fish species was analysed, the majority from farmed Norwegian salmon. MLVA distributed 76% of the isolates across three major clonal complexes (CC1, CC2 and CC3), with the remaining forming minor clusters and singletons. While 90% of the salmon isolates belong to either CC1, CC2 or CC3, only 20% of the isolates recovered from other fish species do so, indicating a considerable degree of host specificity. We further highlight a series of ‘clonal shifts’ amongst Norwegian salmon isolates over the 35-year sampling period, with CC1 showing exclusive predominance prior to the emergence of CC2, which was later supplanted by CC3, before the recent re-emergence of CC1. Apparently, these shifts have rapidly swept the entire Norwegian coastline and conceivably, as suggested by typing of a small number of non-Norwegian isolates, the Northeast Atlantic region as a whole. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Sørgaard, Martin Sveinsson, Karoline Overn Patel, Sonal Nilsen, Hanne Katrine Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan John Gulla, Snorre |
spellingShingle |
Sørgaard, Martin Sveinsson, Karoline Overn Patel, Sonal Nilsen, Hanne Katrine Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan John Gulla, Snorre MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming |
author_facet |
Sørgaard, Martin Sveinsson, Karoline Overn Patel, Sonal Nilsen, Hanne Katrine Olsen, Anne Berit Vaagnes, Øyvind Colquhoun, Duncan John Gulla, Snorre |
author_sort |
Sørgaard, Martin |
title |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming |
title_short |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming |
title_full |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming |
title_fullStr |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming |
title_full_unstemmed |
MLVA genotyping of Moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in Norwegian salmon farming |
title_sort |
mlva genotyping of moritella viscosa reveals serial emergence of novel, host-specific clonal complexes in norwegian salmon farming |
publisher |
Wiley |
publishDate |
2023 |
url |
https://hdl.handle.net/11250/3083300 https://doi.org/10.1111/jfd.13766 |
genre |
Atlantic salmon Northeast Atlantic Salmo salar |
genre_facet |
Atlantic salmon Northeast Atlantic Salmo salar |
op_source |
Journal of Fish Diseases 535-543 46 5 |
op_relation |
urn:issn:0140-7775 https://hdl.handle.net/11250/3083300 https://doi.org/10.1111/jfd.13766 cristin:2145160 Journal of Fish Diseases. 2023, 46 (5), 535-543. |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2023 The Authors. |
op_doi |
https://doi.org/10.1111/jfd.13766 |
container_title |
Journal of Fish Diseases |
container_volume |
46 |
container_issue |
5 |
container_start_page |
535 |
op_container_end_page |
543 |
_version_ |
1776199229015851008 |