qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture
Although a number of genetically diverse Yersinia ruckeri strains are present in Norwegian aquaculture environments, most if not all outbreaks of yersiniosis in Atlantic salmon in Norway are associated with a single specific genetic lineage of serotype O1, termed clonal complex 1. To investigate the...
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Online Access: | https://hdl.handle.net/11250/3061366 https://doi.org/10.1111/jfd.13656 |
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ftunivbergen:oai:bora.uib.no:11250/3061366 2023-05-15T15:32:24+02:00 qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture Riborg, Andreas Ekroll Gulla, Snorre Strand, David Wiik-Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan John 2022 application/pdf https://hdl.handle.net/11250/3061366 https://doi.org/10.1111/jfd.13656 eng eng Wiley urn:issn:0140-7775 https://hdl.handle.net/11250/3061366 https://doi.org/10.1111/jfd.13656 cristin:2048333 Journal of Fish Diseases. 2022, 45 (8), 1211-1224. Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no Copyright 2022 The Author(s) Journal of Fish Diseases 1211-1224 45 8 Journal article Peer reviewed 2022 ftunivbergen https://doi.org/10.1111/jfd.13656 2023-04-05T23:05:49Z Although a number of genetically diverse Yersinia ruckeri strains are present in Norwegian aquaculture environments, most if not all outbreaks of yersiniosis in Atlantic salmon in Norway are associated with a single specific genetic lineage of serotype O1, termed clonal complex 1. To investigate the presence and spread of virulent and putatively avirulent strains in Norwegian salmon farms, PCR assays specific for Y. ruckeri (species level) and Y. ruckeri clonal complex 1 were developed. Following extensive screening of water and biofilm, the widespread prevalence of putatively avirulent Y. ruckeri strains was confirmed in freshwater salmon hatcheries, while Y. ruckeri clonal complex 1 was found in fewer farms. The formalin-killed bacterin yersiniosis vaccine was detected in environmental samples by both PCR assays for several weeks post-vaccination. It is thus important to interpret results from recently vaccinated fish with great care. Moreover, field studies and laboratory trials confirmed that stressful management procedures may result in increased shedding of Y. ruckeri by sub-clinically infected fish. Analysis of sea water sampled throughout thermal delousing procedures proved effective for detection of Y. ruckeri in sub-clinically infected populations. publishedVersion Article in Journal/Newspaper Atlantic salmon University of Bergen: Bergen Open Research Archive (BORA-UiB) Norway Journal of Fish Diseases 45 8 1211 1224 |
institution |
Open Polar |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
language |
English |
description |
Although a number of genetically diverse Yersinia ruckeri strains are present in Norwegian aquaculture environments, most if not all outbreaks of yersiniosis in Atlantic salmon in Norway are associated with a single specific genetic lineage of serotype O1, termed clonal complex 1. To investigate the presence and spread of virulent and putatively avirulent strains in Norwegian salmon farms, PCR assays specific for Y. ruckeri (species level) and Y. ruckeri clonal complex 1 were developed. Following extensive screening of water and biofilm, the widespread prevalence of putatively avirulent Y. ruckeri strains was confirmed in freshwater salmon hatcheries, while Y. ruckeri clonal complex 1 was found in fewer farms. The formalin-killed bacterin yersiniosis vaccine was detected in environmental samples by both PCR assays for several weeks post-vaccination. It is thus important to interpret results from recently vaccinated fish with great care. Moreover, field studies and laboratory trials confirmed that stressful management procedures may result in increased shedding of Y. ruckeri by sub-clinically infected fish. Analysis of sea water sampled throughout thermal delousing procedures proved effective for detection of Y. ruckeri in sub-clinically infected populations. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Riborg, Andreas Ekroll Gulla, Snorre Strand, David Wiik-Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan John |
spellingShingle |
Riborg, Andreas Ekroll Gulla, Snorre Strand, David Wiik-Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan John qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
author_facet |
Riborg, Andreas Ekroll Gulla, Snorre Strand, David Wiik-Nielsen, Jannicke Rønneseth, Anita Welch, Timothy J. Spilsberg, Bjørn Colquhoun, Duncan John |
author_sort |
Riborg, Andreas Ekroll |
title |
qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_short |
qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_full |
qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_fullStr |
qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_full_unstemmed |
qPCR screening for Yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in Norwegian aquaculture |
title_sort |
qpcr screening for yersinia ruckeri clonal complex 1 against a background of putatively avirulent strains in norwegian aquaculture |
publisher |
Wiley |
publishDate |
2022 |
url |
https://hdl.handle.net/11250/3061366 https://doi.org/10.1111/jfd.13656 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Journal of Fish Diseases 1211-1224 45 8 |
op_relation |
urn:issn:0140-7775 https://hdl.handle.net/11250/3061366 https://doi.org/10.1111/jfd.13656 cristin:2048333 Journal of Fish Diseases. 2022, 45 (8), 1211-1224. |
op_rights |
Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no Copyright 2022 The Author(s) |
op_doi |
https://doi.org/10.1111/jfd.13656 |
container_title |
Journal of Fish Diseases |
container_volume |
45 |
container_issue |
8 |
container_start_page |
1211 |
op_container_end_page |
1224 |
_version_ |
1766362903055695872 |