Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters

The high-affinity/low-capacity system Slc15a2 (PepT2) is responsible for the reuptake of di/tripeptides from the renal proximal tubule, but it also operates in many other tissues and organs. Information regarding PepT2 in teleost fish is limited and, to date, functional data are available from the z...

Full description

Bibliographic Details
Published in:The Journal of Physiology
Main Authors: Vacca, Francesca, Gomes, Ana S., Murashita, Koji, Cinquetti, Raffella, Roseti, Cristina, Barca, Amilcare, Rønnestad, Ivar, Verri, Tiziano, Bossi, Elena
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2022
Subjects:
Online Access:https://hdl.handle.net/11250/3037854
https://doi.org/10.1113/JP282781
id ftunivbergen:oai:bora.uib.no:11250/3037854
record_format openpolar
spelling ftunivbergen:oai:bora.uib.no:11250/3037854 2023-05-15T15:30:24+02:00 Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters Vacca, Francesca Gomes, Ana S. Murashita, Koji Cinquetti, Raffella Roseti, Cristina Barca, Amilcare Rønnestad, Ivar Verri, Tiziano Bossi, Elena 2022 application/pdf https://hdl.handle.net/11250/3037854 https://doi.org/10.1113/JP282781 eng eng Wiley urn:issn:0022-3751 https://hdl.handle.net/11250/3037854 https://doi.org/10.1113/JP282781 cristin:2030029 Journal of Physiology. 2022, 600 (10), 2377-2400. Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no Copyright 2022 the authors Journal of Physiology 2377-2400 600 10 Journal article Peer reviewed 2022 ftunivbergen https://doi.org/10.1113/JP282781 2023-03-14T17:42:28Z The high-affinity/low-capacity system Slc15a2 (PepT2) is responsible for the reuptake of di/tripeptides from the renal proximal tubule, but it also operates in many other tissues and organs. Information regarding PepT2 in teleost fish is limited and, to date, functional data are available from the zebrafish (Danio rerio) only. Here, we report the identification of two slc15a2 genes in the Atlantic salmon (Salmo salar) genome, namely slc15a2a and slc15a2b. The two encoded PepT2 proteins share 87% identity and resemble both structurally and functionally the canonical vertebrate PepT2 system. The mRNA tissue distribution analyses reveal a widespread distribution of slc15a2a transcripts, being more abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and the distal part of the gastrointestinal tract. The function of the two transporters was investigated by heterologous expression in Xenopus laevis oocytes and two-electrode voltage-clamp recordings of transport and presteady-state currents. Both PepT2a and PepT2b in the presence of Gly-Gln elicit pH-dependent and Na+ independent inward currents. The biophysical and kinetic analysis of the recorded currents defined the transport properties, confirming that the two Atlantic salmon PepT2 proteins behave as high-affinity/low-capacity transporters. The recent structures and the previous kinetic schemes of rat and human PepT2 qualitatively account for the characteristics of the two Atlantic salmon proteins. This study is the first to report on the functional expression of two PepT2-type transporters that operate in the same vertebrate organism as a result of (a) gene duplication process(es). publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) The Journal of Physiology 600 10 2377 2400
institution Open Polar
collection University of Bergen: Bergen Open Research Archive (BORA-UiB)
op_collection_id ftunivbergen
language English
description The high-affinity/low-capacity system Slc15a2 (PepT2) is responsible for the reuptake of di/tripeptides from the renal proximal tubule, but it also operates in many other tissues and organs. Information regarding PepT2 in teleost fish is limited and, to date, functional data are available from the zebrafish (Danio rerio) only. Here, we report the identification of two slc15a2 genes in the Atlantic salmon (Salmo salar) genome, namely slc15a2a and slc15a2b. The two encoded PepT2 proteins share 87% identity and resemble both structurally and functionally the canonical vertebrate PepT2 system. The mRNA tissue distribution analyses reveal a widespread distribution of slc15a2a transcripts, being more abundant in the brain and gills, while slc15a2b transcripts are mainly expressed in the kidney and the distal part of the gastrointestinal tract. The function of the two transporters was investigated by heterologous expression in Xenopus laevis oocytes and two-electrode voltage-clamp recordings of transport and presteady-state currents. Both PepT2a and PepT2b in the presence of Gly-Gln elicit pH-dependent and Na+ independent inward currents. The biophysical and kinetic analysis of the recorded currents defined the transport properties, confirming that the two Atlantic salmon PepT2 proteins behave as high-affinity/low-capacity transporters. The recent structures and the previous kinetic schemes of rat and human PepT2 qualitatively account for the characteristics of the two Atlantic salmon proteins. This study is the first to report on the functional expression of two PepT2-type transporters that operate in the same vertebrate organism as a result of (a) gene duplication process(es). publishedVersion
format Article in Journal/Newspaper
author Vacca, Francesca
Gomes, Ana S.
Murashita, Koji
Cinquetti, Raffella
Roseti, Cristina
Barca, Amilcare
Rønnestad, Ivar
Verri, Tiziano
Bossi, Elena
spellingShingle Vacca, Francesca
Gomes, Ana S.
Murashita, Koji
Cinquetti, Raffella
Roseti, Cristina
Barca, Amilcare
Rønnestad, Ivar
Verri, Tiziano
Bossi, Elena
Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters
author_facet Vacca, Francesca
Gomes, Ana S.
Murashita, Koji
Cinquetti, Raffella
Roseti, Cristina
Barca, Amilcare
Rønnestad, Ivar
Verri, Tiziano
Bossi, Elena
author_sort Vacca, Francesca
title Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters
title_short Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters
title_full Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters
title_fullStr Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters
title_full_unstemmed Functional characterization of Atlantic salmon (Salmo salar L.) PepT2 transporters
title_sort functional characterization of atlantic salmon (salmo salar l.) pept2 transporters
publisher Wiley
publishDate 2022
url https://hdl.handle.net/11250/3037854
https://doi.org/10.1113/JP282781
genre Atlantic salmon
Salmo salar
genre_facet Atlantic salmon
Salmo salar
op_source Journal of Physiology
2377-2400
600
10
op_relation urn:issn:0022-3751
https://hdl.handle.net/11250/3037854
https://doi.org/10.1113/JP282781
cristin:2030029
Journal of Physiology. 2022, 600 (10), 2377-2400.
op_rights Attribution-NonCommercial-NoDerivatives 4.0 Internasjonal
http://creativecommons.org/licenses/by-nc-nd/4.0/deed.no
Copyright 2022 the authors
op_doi https://doi.org/10.1113/JP282781
container_title The Journal of Physiology
container_volume 600
container_issue 10
container_start_page 2377
op_container_end_page 2400
_version_ 1766360849575837696