Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a
Polycyclic aromatic hydrocarbons (PAHs) are among the most toxic and bioavailable components found in petroleum and represent a high risk to aquatic organisms. The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that mediates the toxicity of 2,3,7,8-tetrachlorodibenzo-p-di...
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Online Access: | https://hdl.handle.net/11250/2832035 https://doi.org/10.1021/acs.est.1c02946 |
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ftunivbergen:oai:bora.uib.no:11250/2832035 2023-05-15T15:26:59+02:00 Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a Lille-Langøy, Roger Jørgensen, Kåre Bredeli Goksøyr, Anders Pampanin, Daniela Maria Sydnes, Magne Olav Karlsen, Odd André 2021 application/pdf https://hdl.handle.net/11250/2832035 https://doi.org/10.1021/acs.est.1c02946 eng eng American Chemical Society urn:issn:0013-936X https://hdl.handle.net/11250/2832035 https://doi.org/10.1021/acs.est.1c02946 cristin:1960036 Environmental Science and Technology. 2021, 55 (22), 15123-15135. Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2021 the authors Environmental Science and Technology 15123-15135 55 22 Journal article Peer reviewed 2021 ftunivbergen https://doi.org/10.1021/acs.est.1c02946 2023-03-14T17:42:20Z Polycyclic aromatic hydrocarbons (PAHs) are among the most toxic and bioavailable components found in petroleum and represent a high risk to aquatic organisms. The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that mediates the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other planar aromatic hydrocarbons, including certain PAHs. Ahr acts as a xenosensor and modulates the transcription of biotransformation genes in vertebrates, such as cytochrome P450 1A (cyp1a). Atlantic cod (Gadus morhua) possesses two Ahr proteins, Ahr1a and Ahr2a, which diverge in their primary structure, tissue-specific expression, ligand affinities, and transactivation profiles. Here, a luciferase reporter gene assay was used to assess the sensitivity of the Atlantic cod Ahrs to 31 polycyclic aromatic compounds (PACs), including two- to five-ring native PAHs, a sulfur-containing heterocyclic PAC, as well as several methylated, methoxylated, and hydroxylated congeners. Notably, most parent compounds, including naphthalene, phenanthrene, and partly, chrysene, did not act as agonists for the Ahrs, while hydroxylated and/or alkylated versions of these PAHs were potent agonists. Importantly, the greater potencies of substituted PAH derivatives and their ubiquitous occurrence in nature emphasize that more knowledge on the toxicity of these environmentally and toxicologically relevant compounds is imperative. publishedVersion Article in Journal/Newspaper atlantic cod Gadus morhua University of Bergen: Bergen Open Research Archive (BORA-UiB) Environmental Science & Technology 55 22 15123 15135 |
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Open Polar |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
Polycyclic aromatic hydrocarbons (PAHs) are among the most toxic and bioavailable components found in petroleum and represent a high risk to aquatic organisms. The aryl hydrocarbon receptor (Ahr) is a ligand-activated transcription factor that mediates the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and other planar aromatic hydrocarbons, including certain PAHs. Ahr acts as a xenosensor and modulates the transcription of biotransformation genes in vertebrates, such as cytochrome P450 1A (cyp1a). Atlantic cod (Gadus morhua) possesses two Ahr proteins, Ahr1a and Ahr2a, which diverge in their primary structure, tissue-specific expression, ligand affinities, and transactivation profiles. Here, a luciferase reporter gene assay was used to assess the sensitivity of the Atlantic cod Ahrs to 31 polycyclic aromatic compounds (PACs), including two- to five-ring native PAHs, a sulfur-containing heterocyclic PAC, as well as several methylated, methoxylated, and hydroxylated congeners. Notably, most parent compounds, including naphthalene, phenanthrene, and partly, chrysene, did not act as agonists for the Ahrs, while hydroxylated and/or alkylated versions of these PAHs were potent agonists. Importantly, the greater potencies of substituted PAH derivatives and their ubiquitous occurrence in nature emphasize that more knowledge on the toxicity of these environmentally and toxicologically relevant compounds is imperative. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Lille-Langøy, Roger Jørgensen, Kåre Bredeli Goksøyr, Anders Pampanin, Daniela Maria Sydnes, Magne Olav Karlsen, Odd André |
spellingShingle |
Lille-Langøy, Roger Jørgensen, Kåre Bredeli Goksøyr, Anders Pampanin, Daniela Maria Sydnes, Magne Olav Karlsen, Odd André Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a |
author_facet |
Lille-Langøy, Roger Jørgensen, Kåre Bredeli Goksøyr, Anders Pampanin, Daniela Maria Sydnes, Magne Olav Karlsen, Odd André |
author_sort |
Lille-Langøy, Roger |
title |
Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a |
title_short |
Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a |
title_full |
Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a |
title_fullStr |
Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a |
title_full_unstemmed |
Substituted Two- To Five-Ring Polycyclic Aromatic Compounds Are Potent Agonists of Atlantic Cod (Gadus morhua) Aryl Hydrocarbon Receptors Ahr1a and Ahr2a |
title_sort |
substituted two- to five-ring polycyclic aromatic compounds are potent agonists of atlantic cod (gadus morhua) aryl hydrocarbon receptors ahr1a and ahr2a |
publisher |
American Chemical Society |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2832035 https://doi.org/10.1021/acs.est.1c02946 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Environmental Science and Technology 15123-15135 55 22 |
op_relation |
urn:issn:0013-936X https://hdl.handle.net/11250/2832035 https://doi.org/10.1021/acs.est.1c02946 cristin:1960036 Environmental Science and Technology. 2021, 55 (22), 15123-15135. |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2021 the authors |
op_doi |
https://doi.org/10.1021/acs.est.1c02946 |
container_title |
Environmental Science & Technology |
container_volume |
55 |
container_issue |
22 |
container_start_page |
15123 |
op_container_end_page |
15135 |
_version_ |
1766357444520312832 |