Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar)
Sensing of amino acids in fish brain, especially branched-chain amino acids (BCAA) like leucine, is involved in regulation of feed intake through different mechanisms. However, there is limited information regarding the possible involvement of mechanisms dependent on amino acid carriers of the solut...
Published in: | Frontiers in Marine Science |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media
2021
|
Subjects: | |
Online Access: | https://hdl.handle.net/11250/2825318 https://doi.org/10.3389/fmars.2021.711508 |
id |
ftunivbergen:oai:bora.uib.no:11250/2825318 |
---|---|
record_format |
openpolar |
spelling |
ftunivbergen:oai:bora.uib.no:11250/2825318 2023-05-15T15:30:55+02:00 Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) Comesaña, Sara Lai, Floriana Jordal, Ann-Elise Olderbakk Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar 2021 application/pdf https://hdl.handle.net/11250/2825318 https://doi.org/10.3389/fmars.2021.711508 eng eng Frontiers Media Norges forskningsråd: 267626 urn:issn:2296-7745 https://hdl.handle.net/11250/2825318 https://doi.org/10.3389/fmars.2021.711508 cristin:1947392 Frontiers in Marine Science. 2021, 8, 711508. Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2021 Comesaña, Lai, Olderbakk Jordal, Verri, Espe, Soengas and Rønnestad. 711508 Frontiers in Marine Science 8 Journal article Peer reviewed 2021 ftunivbergen https://doi.org/10.3389/fmars.2021.711508 2023-03-14T17:42:27Z Sensing of amino acids in fish brain, especially branched-chain amino acids (BCAA) like leucine, is involved in regulation of feed intake through different mechanisms. However, there is limited information regarding the possible involvement of mechanisms dependent on amino acid carriers of the solute carrier families (SLC) known to be key regulators of intracellular leucine concentration, namely L-type amino acid transporter 1 (LAT1), and sodium-dependent neutral amino acid transporter 2 (SNAT2) and 9,(SNAT9), for which evidence of their participation is available in mammals. Comparative analysis amongst sequences revealed a complex pattern of paralogues in Atlantic salmon, for LAT1 (slc7a5aa, slc7a5ab, slc7a5ba, slc7a5bb, slc7a5ca, and slc7a5cb), SNAT2 (slc38a2a and slc38a2b) and SNAT9 (slc38a9). After establishing phylogenetic relationships of the different paralogues evaluated, samples of the selected brain areas were taken from Atlantic salmon to assess tissue distribution of transcripts. In an additional experiment, fish were fed two diets with different levels of leucine (high leucine: 35 g/kg vs. control leucine: 27.3 g/kg). The high leucine diet resulted in lower feed intake and increased mRNA abundance of specific paralogues of LAT1 (slc7a5aa, slc7a5ab, and slc7a5bb) and SNAT2 (slc38a2a and slc38a2b) though apparently not for SNAT9 in brain areas like hypothalamus and telencephalon involved in food intake regulation. The results obtained suggest a role for members of the SLC family in the anorectic effect of leucine and thus their involvement as additional amino acid sensing mechanism not characterised so far in fish regulation of feed intake. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) Frontiers in Marine Science 8 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
Sensing of amino acids in fish brain, especially branched-chain amino acids (BCAA) like leucine, is involved in regulation of feed intake through different mechanisms. However, there is limited information regarding the possible involvement of mechanisms dependent on amino acid carriers of the solute carrier families (SLC) known to be key regulators of intracellular leucine concentration, namely L-type amino acid transporter 1 (LAT1), and sodium-dependent neutral amino acid transporter 2 (SNAT2) and 9,(SNAT9), for which evidence of their participation is available in mammals. Comparative analysis amongst sequences revealed a complex pattern of paralogues in Atlantic salmon, for LAT1 (slc7a5aa, slc7a5ab, slc7a5ba, slc7a5bb, slc7a5ca, and slc7a5cb), SNAT2 (slc38a2a and slc38a2b) and SNAT9 (slc38a9). After establishing phylogenetic relationships of the different paralogues evaluated, samples of the selected brain areas were taken from Atlantic salmon to assess tissue distribution of transcripts. In an additional experiment, fish were fed two diets with different levels of leucine (high leucine: 35 g/kg vs. control leucine: 27.3 g/kg). The high leucine diet resulted in lower feed intake and increased mRNA abundance of specific paralogues of LAT1 (slc7a5aa, slc7a5ab, and slc7a5bb) and SNAT2 (slc38a2a and slc38a2b) though apparently not for SNAT9 in brain areas like hypothalamus and telencephalon involved in food intake regulation. The results obtained suggest a role for members of the SLC family in the anorectic effect of leucine and thus their involvement as additional amino acid sensing mechanism not characterised so far in fish regulation of feed intake. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Comesaña, Sara Lai, Floriana Jordal, Ann-Elise Olderbakk Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar |
spellingShingle |
Comesaña, Sara Lai, Floriana Jordal, Ann-Elise Olderbakk Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
author_facet |
Comesaña, Sara Lai, Floriana Jordal, Ann-Elise Olderbakk Verri, Tiziano Espe, Marit Soengas, José L. Rønnestad, Ivar |
author_sort |
Comesaña, Sara |
title |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_short |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_full |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_fullStr |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_full_unstemmed |
Amino Acid Carriers of the Solute Carrier Families 7 (SLC7) and 38 (SLC38) Are Involved in Leucine Sensing in the Brain of Atlantic Salmon (Salmo salar) |
title_sort |
amino acid carriers of the solute carrier families 7 (slc7) and 38 (slc38) are involved in leucine sensing in the brain of atlantic salmon (salmo salar) |
publisher |
Frontiers Media |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2825318 https://doi.org/10.3389/fmars.2021.711508 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
711508 Frontiers in Marine Science 8 |
op_relation |
Norges forskningsråd: 267626 urn:issn:2296-7745 https://hdl.handle.net/11250/2825318 https://doi.org/10.3389/fmars.2021.711508 cristin:1947392 Frontiers in Marine Science. 2021, 8, 711508. |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2021 Comesaña, Lai, Olderbakk Jordal, Verri, Espe, Soengas and Rønnestad. |
op_doi |
https://doi.org/10.3389/fmars.2021.711508 |
container_title |
Frontiers in Marine Science |
container_volume |
8 |
_version_ |
1766361403036270592 |