Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic
This study investigated concentrations of phthalates (diesters of phthalic acids) in blubber/adipose tissue of blue whales (Balaenoptera musculus), fin whales (Balaenoptera physalus), bowhead whales (Balaena mysticetus) and polar bears (Ursus maritimus) sampled in the Svalbard Archipelago (extending...
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Online Access: | https://hdl.handle.net/11250/2766783 https://doi.org/10.1016/j.envint.2021.106458 |
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ftunivbergen:oai:bora.uib.no:11250/2766783 2023-05-15T15:02:18+02:00 Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic Routti, Heli Anna Irmeli Harju, Mikael Lühmann, Katharina Aars, Jon Ask, Amalie Goksøyr, Anders Kovacs, Kit M. Lydersen, Christian 2021 application/pdf https://hdl.handle.net/11250/2766783 https://doi.org/10.1016/j.envint.2021.106458 eng eng Elsevier Norges forskningsråd: 244488 urn:issn:0160-4120 https://hdl.handle.net/11250/2766783 https://doi.org/10.1016/j.envint.2021.106458 cristin:1898715 Environment International. 2021, 152, 106458 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2021 The Authors 106458 Environment International 152 Journal article Peer reviewed 2021 ftunivbergen https://doi.org/10.1016/j.envint.2021.106458 2023-03-14T17:40:23Z This study investigated concentrations of phthalates (diesters of phthalic acids) in blubber/adipose tissue of blue whales (Balaenoptera musculus), fin whales (Balaenoptera physalus), bowhead whales (Balaena mysticetus) and polar bears (Ursus maritimus) sampled in the Svalbard Archipelago (extending westward in the case of bowhead whales). Additionally, total concentrations (free and conjugated forms) of eight phthalate monoester metabolites were analysed in plasma of polar bears. Bis(2-ethylhexyl) phthalate (DEHP) was the only phthalate quantified among the 12 phthalates investigated. This compound was present in 6/7 fin whale samples, 4/7 blue whale samples, 2/5 bowhead whale samples and 1/12 polar bear samples. DEHP concentrations ranged from <20–398 ng/g wet weight. Phthalate metabolites, mono-n-butyl phthalate and monoisobutyl phthalate, were found in low concentrations (<1.2 ng/mL) in some of the polar bear samples. In vitro reporter gene assays were used to assess transcriptional activity of fin whale peroxisome proliferator-activated receptor gamma (PPARG), glucocorticoid receptor (GR) and the thyroid hormone receptor beta (THRB) by DEHP and diisononyl phthalate (DiNP). Due to the high degree of similarity of the ligand binding domain in the THRB and PPARG among whales, polar bears and humans, the transactivation results also apply for these species. DEHP showed both agonistic and antagonistic effects towards whale THRB at considerably higher concentrations than measured in the study animals; DiNP was a weak agonist of whale THRB. No significant agonistic or antagonistic effects were detected for DEHP or DiNP for whale PPARG, whereas DEHP and DiNP decreased basal luciferase activity mediated by whale GR at several test concentrations. In conclusion, DEHP was detected in the blubber of marine mammals from the Norwegian Arctic and it appears to have potential to modulate the transcriptional activity of whale THRB, but current DEHP concentrations do not modulate the function of the studied nuclear ... Article in Journal/Newspaper Arctic Balaena mysticetus Balaenoptera musculus Balaenoptera physalus Blue whale bowhead whale Fin whale polar bear Svalbard Ursus maritimus University of Bergen: Bergen Open Research Archive (BORA-UiB) Arctic Svalbard Svalbard Archipelago Environment International 152 106458 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
This study investigated concentrations of phthalates (diesters of phthalic acids) in blubber/adipose tissue of blue whales (Balaenoptera musculus), fin whales (Balaenoptera physalus), bowhead whales (Balaena mysticetus) and polar bears (Ursus maritimus) sampled in the Svalbard Archipelago (extending westward in the case of bowhead whales). Additionally, total concentrations (free and conjugated forms) of eight phthalate monoester metabolites were analysed in plasma of polar bears. Bis(2-ethylhexyl) phthalate (DEHP) was the only phthalate quantified among the 12 phthalates investigated. This compound was present in 6/7 fin whale samples, 4/7 blue whale samples, 2/5 bowhead whale samples and 1/12 polar bear samples. DEHP concentrations ranged from <20–398 ng/g wet weight. Phthalate metabolites, mono-n-butyl phthalate and monoisobutyl phthalate, were found in low concentrations (<1.2 ng/mL) in some of the polar bear samples. In vitro reporter gene assays were used to assess transcriptional activity of fin whale peroxisome proliferator-activated receptor gamma (PPARG), glucocorticoid receptor (GR) and the thyroid hormone receptor beta (THRB) by DEHP and diisononyl phthalate (DiNP). Due to the high degree of similarity of the ligand binding domain in the THRB and PPARG among whales, polar bears and humans, the transactivation results also apply for these species. DEHP showed both agonistic and antagonistic effects towards whale THRB at considerably higher concentrations than measured in the study animals; DiNP was a weak agonist of whale THRB. No significant agonistic or antagonistic effects were detected for DEHP or DiNP for whale PPARG, whereas DEHP and DiNP decreased basal luciferase activity mediated by whale GR at several test concentrations. In conclusion, DEHP was detected in the blubber of marine mammals from the Norwegian Arctic and it appears to have potential to modulate the transcriptional activity of whale THRB, but current DEHP concentrations do not modulate the function of the studied nuclear ... |
format |
Article in Journal/Newspaper |
author |
Routti, Heli Anna Irmeli Harju, Mikael Lühmann, Katharina Aars, Jon Ask, Amalie Goksøyr, Anders Kovacs, Kit M. Lydersen, Christian |
spellingShingle |
Routti, Heli Anna Irmeli Harju, Mikael Lühmann, Katharina Aars, Jon Ask, Amalie Goksøyr, Anders Kovacs, Kit M. Lydersen, Christian Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic |
author_facet |
Routti, Heli Anna Irmeli Harju, Mikael Lühmann, Katharina Aars, Jon Ask, Amalie Goksøyr, Anders Kovacs, Kit M. Lydersen, Christian |
author_sort |
Routti, Heli Anna Irmeli |
title |
Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic |
title_short |
Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic |
title_full |
Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic |
title_fullStr |
Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic |
title_full_unstemmed |
Concentrations and endocrine disruptive potential of phthalates in marine mammals from the Norwegian Arctic |
title_sort |
concentrations and endocrine disruptive potential of phthalates in marine mammals from the norwegian arctic |
publisher |
Elsevier |
publishDate |
2021 |
url |
https://hdl.handle.net/11250/2766783 https://doi.org/10.1016/j.envint.2021.106458 |
geographic |
Arctic Svalbard Svalbard Archipelago |
geographic_facet |
Arctic Svalbard Svalbard Archipelago |
genre |
Arctic Balaena mysticetus Balaenoptera musculus Balaenoptera physalus Blue whale bowhead whale Fin whale polar bear Svalbard Ursus maritimus |
genre_facet |
Arctic Balaena mysticetus Balaenoptera musculus Balaenoptera physalus Blue whale bowhead whale Fin whale polar bear Svalbard Ursus maritimus |
op_source |
106458 Environment International 152 |
op_relation |
Norges forskningsråd: 244488 urn:issn:0160-4120 https://hdl.handle.net/11250/2766783 https://doi.org/10.1016/j.envint.2021.106458 cristin:1898715 Environment International. 2021, 152, 106458 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2021 The Authors |
op_doi |
https://doi.org/10.1016/j.envint.2021.106458 |
container_title |
Environment International |
container_volume |
152 |
container_start_page |
106458 |
_version_ |
1766334267005075456 |