Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome
Background: Blood-feeding is a common strategy among parasitizing arthropods, including the ectoparasitic salmon louse (Lepeophtheirus salmonis), feeding off its salmon host’s skin and blood. Blood is rich in nutrients, among these iron and heme. These are essential molecules for the louse, yet thei...
Published in: | Parasites & Vectors |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BioMed Central
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/11250/2764496 https://doi.org/10.1186/s13071-020-04096-0 |
id |
ftunivbergen:oai:bora.uib.no:11250/2764496 |
---|---|
record_format |
openpolar |
spelling |
ftunivbergen:oai:bora.uib.no:11250/2764496 2023-05-15T15:32:52+02:00 Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome Heggland, Erna Irene Dondrup, Michael Nilsen, Frank Eichner, Christiane 2020-05-06 application/pdf https://hdl.handle.net/11250/2764496 https://doi.org/10.1186/s13071-020-04096-0 eng eng BioMed Central urn:issn:1756-3305 https://hdl.handle.net/11250/2764496 https://doi.org/10.1186/s13071-020-04096-0 cristin:1893208 Parasites & Vectors. 2020, 13, 225. Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright the authors 225 Parasites & Vectors 13 Journal article Peer reviewed 2020 ftunivbergen https://doi.org/10.1186/s13071-020-04096-0 2023-03-14T17:43:12Z Background: Blood-feeding is a common strategy among parasitizing arthropods, including the ectoparasitic salmon louse (Lepeophtheirus salmonis), feeding off its salmon host’s skin and blood. Blood is rich in nutrients, among these iron and heme. These are essential molecules for the louse, yet their oxidative properties render them toxic to cells if not handled appropriately. Blood-feeding might therefore alter parasite gene expression. Methods: We infected Atlantic salmon with salmon louse copepodids and sampled the lice in two different experiments at day 10 and 18 post-infestation. Parasite development and presence of host blood in their intestines were determined. Lice of similar instar age sampled from body parts with differential access to blood, namely from gills versus lice from skin epidermis, were analysed for gene expression by RNA-sequencing in samples taken at day 10 for both experiments and at day 18 for one of the experiments. Results: We found that lice started feeding on blood when becoming mobile preadults if sitting on the fish body; however, they may initiate blood-feeding at the chalimus I stage if attached to gills. Lice attached to gills develop at a slower rate. By differential expression analysis, we found 355 transcripts elevated in lice sampled from gills and 202 transcripts elevated in lice sampled from skin consistent in all samplings. Genes annotated with “peptidase activity” were among the ones elevated in lice sampled from gills, while in the other group genes annotated with “phosphorylation” and “phosphatase” were pervasive. Transcripts elevated in lice sampled from gills were often genes relatively highly expressed in the louse intestine compared with other tissues, while this was not the case for transcripts elevated in lice sampled from skin. In both groups, more than half of the transcripts were from genes more highly expressed after attachment. Conclusions: Gill settlement results in an alteration in gene expression and a premature onset of blood-feeding likely causes the ... Article in Journal/Newspaper Atlantic salmon University of Bergen: Bergen Open Research Archive (BORA-UiB) The Louse ENVELOPE(-56.415,-56.415,51.700,51.700) Parasites & Vectors 13 1 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
Background: Blood-feeding is a common strategy among parasitizing arthropods, including the ectoparasitic salmon louse (Lepeophtheirus salmonis), feeding off its salmon host’s skin and blood. Blood is rich in nutrients, among these iron and heme. These are essential molecules for the louse, yet their oxidative properties render them toxic to cells if not handled appropriately. Blood-feeding might therefore alter parasite gene expression. Methods: We infected Atlantic salmon with salmon louse copepodids and sampled the lice in two different experiments at day 10 and 18 post-infestation. Parasite development and presence of host blood in their intestines were determined. Lice of similar instar age sampled from body parts with differential access to blood, namely from gills versus lice from skin epidermis, were analysed for gene expression by RNA-sequencing in samples taken at day 10 for both experiments and at day 18 for one of the experiments. Results: We found that lice started feeding on blood when becoming mobile preadults if sitting on the fish body; however, they may initiate blood-feeding at the chalimus I stage if attached to gills. Lice attached to gills develop at a slower rate. By differential expression analysis, we found 355 transcripts elevated in lice sampled from gills and 202 transcripts elevated in lice sampled from skin consistent in all samplings. Genes annotated with “peptidase activity” were among the ones elevated in lice sampled from gills, while in the other group genes annotated with “phosphorylation” and “phosphatase” were pervasive. Transcripts elevated in lice sampled from gills were often genes relatively highly expressed in the louse intestine compared with other tissues, while this was not the case for transcripts elevated in lice sampled from skin. In both groups, more than half of the transcripts were from genes more highly expressed after attachment. Conclusions: Gill settlement results in an alteration in gene expression and a premature onset of blood-feeding likely causes the ... |
format |
Article in Journal/Newspaper |
author |
Heggland, Erna Irene Dondrup, Michael Nilsen, Frank Eichner, Christiane |
spellingShingle |
Heggland, Erna Irene Dondrup, Michael Nilsen, Frank Eichner, Christiane Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome |
author_facet |
Heggland, Erna Irene Dondrup, Michael Nilsen, Frank Eichner, Christiane |
author_sort |
Heggland, Erna Irene |
title |
Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome |
title_short |
Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome |
title_full |
Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome |
title_fullStr |
Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome |
title_full_unstemmed |
Host gill attachment causes blood-feeding by the salmon louse (Lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome |
title_sort |
host gill attachment causes blood-feeding by the salmon louse (lepeophtheirus salmonis) chalimus larvae and alters parasite development and transcriptome |
publisher |
BioMed Central |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2764496 https://doi.org/10.1186/s13071-020-04096-0 |
long_lat |
ENVELOPE(-56.415,-56.415,51.700,51.700) |
geographic |
The Louse |
geographic_facet |
The Louse |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
225 Parasites & Vectors 13 |
op_relation |
urn:issn:1756-3305 https://hdl.handle.net/11250/2764496 https://doi.org/10.1186/s13071-020-04096-0 cristin:1893208 Parasites & Vectors. 2020, 13, 225. |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright the authors |
op_doi |
https://doi.org/10.1186/s13071-020-04096-0 |
container_title |
Parasites & Vectors |
container_volume |
13 |
container_issue |
1 |
_version_ |
1766363351915429888 |