Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site
In the present study, a previously capped waste disposal site at Kollevåg (Norway) was selected to study the effects of contaminant leakage on biomarkers associated with Atlantic cod (Gadus morhua) reproductive endocrinology and development. Immature cod were caged for 6 weeks at 3 locations, select...
Published in: | Environmental Research |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Elsevier
2020
|
Subjects: | |
Online Access: | https://hdl.handle.net/11250/2763108 https://doi.org/10.1016/j.envres.2020.109906 |
id |
ftunivbergen:oai:bora.uib.no:11250/2763108 |
---|---|
record_format |
openpolar |
spelling |
ftunivbergen:oai:bora.uib.no:11250/2763108 2023-05-15T15:27:21+02:00 Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site Khan, Essa Ahsan Zhang, Xiaokang Hanna, Eileen Marie Bartosova, Zdenka Yadetie, Fekadu Jonassen, Inge Goksøyr, Anders Arukwe, Augustine 2020 application/pdf https://hdl.handle.net/11250/2763108 https://doi.org/10.1016/j.envres.2020.109906 eng eng Elsevier urn:issn:0013-9351 https://hdl.handle.net/11250/2763108 https://doi.org/10.1016/j.envres.2020.109906 cristin:1827932 Environmental Research. 2020, 189, 109906 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2020 The Authors 109906 Environmental Research 189:109906 189 Journal article Peer reviewed 2020 ftunivbergen https://doi.org/10.1016/j.envres.2020.109906 2023-03-14T17:38:49Z In the present study, a previously capped waste disposal site at Kollevåg (Norway) was selected to study the effects of contaminant leakage on biomarkers associated with Atlantic cod (Gadus morhua) reproductive endocrinology and development. Immature cod were caged for 6 weeks at 3 locations, selected to achieve a spatial gradient of contamination, and compared to a reference station. Quantitative transcriptomic, and lipidomic analysis was used to evaluate the effects of the potential complex contaminant mixture on ovarian developmental and endocrine physiology. The number of expressed transcripts, with 0.75 log2-fold differential expression or more, varied among stations and paralleled the severity of contamination. Particularly, significant bioaccumulation of ∑PCB-7, ∑DDTs and ∑PBDEs were observed at station 1, compared to the other station, including the reference station. Respectively 1416, 698 and 719 differentially expressed genes (DEGs), were observed at stations 1, 2 and 3, compared to the reference station, with transcripts belonging to steroid hormone synthesis pathway being significantly upregulation. Transcription factors such as esr2 and ahr2 were increased at all three stations, with highest fold-change at Station 1. MetaCore pathway maps identified affected pathways that are involved in ovarian physiology, where some unique pathways were significantly affected at each station. For the lipidomics, sphingolipid metabolism was particularly affected at station 1, and these effects paralleled the high contaminant burden at this station. Overall, our findings showed a novel and direct association between contaminant burden and ovarian toxicological and endocrine physiological responses in cod caged at the capped Kollevåg waste disposal site. publishedVersion Article in Journal/Newspaper atlantic cod Gadus morhua University of Bergen: Bergen Open Research Archive (BORA-UiB) Norway Environmental Research 189 109906 |
institution |
Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
op_collection_id |
ftunivbergen |
language |
English |
description |
In the present study, a previously capped waste disposal site at Kollevåg (Norway) was selected to study the effects of contaminant leakage on biomarkers associated with Atlantic cod (Gadus morhua) reproductive endocrinology and development. Immature cod were caged for 6 weeks at 3 locations, selected to achieve a spatial gradient of contamination, and compared to a reference station. Quantitative transcriptomic, and lipidomic analysis was used to evaluate the effects of the potential complex contaminant mixture on ovarian developmental and endocrine physiology. The number of expressed transcripts, with 0.75 log2-fold differential expression or more, varied among stations and paralleled the severity of contamination. Particularly, significant bioaccumulation of ∑PCB-7, ∑DDTs and ∑PBDEs were observed at station 1, compared to the other station, including the reference station. Respectively 1416, 698 and 719 differentially expressed genes (DEGs), were observed at stations 1, 2 and 3, compared to the reference station, with transcripts belonging to steroid hormone synthesis pathway being significantly upregulation. Transcription factors such as esr2 and ahr2 were increased at all three stations, with highest fold-change at Station 1. MetaCore pathway maps identified affected pathways that are involved in ovarian physiology, where some unique pathways were significantly affected at each station. For the lipidomics, sphingolipid metabolism was particularly affected at station 1, and these effects paralleled the high contaminant burden at this station. Overall, our findings showed a novel and direct association between contaminant burden and ovarian toxicological and endocrine physiological responses in cod caged at the capped Kollevåg waste disposal site. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Khan, Essa Ahsan Zhang, Xiaokang Hanna, Eileen Marie Bartosova, Zdenka Yadetie, Fekadu Jonassen, Inge Goksøyr, Anders Arukwe, Augustine |
spellingShingle |
Khan, Essa Ahsan Zhang, Xiaokang Hanna, Eileen Marie Bartosova, Zdenka Yadetie, Fekadu Jonassen, Inge Goksøyr, Anders Arukwe, Augustine Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site |
author_facet |
Khan, Essa Ahsan Zhang, Xiaokang Hanna, Eileen Marie Bartosova, Zdenka Yadetie, Fekadu Jonassen, Inge Goksøyr, Anders Arukwe, Augustine |
author_sort |
Khan, Essa Ahsan |
title |
Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site |
title_short |
Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site |
title_full |
Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site |
title_fullStr |
Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site |
title_full_unstemmed |
Quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in Atlantic cod (Gadus morhua) caged at a capped marine waste disposal site |
title_sort |
quantitative transcriptomics, and lipidomics in evaluating ovarian developmental effects in atlantic cod (gadus morhua) caged at a capped marine waste disposal site |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2763108 https://doi.org/10.1016/j.envres.2020.109906 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
109906 Environmental Research 189:109906 189 |
op_relation |
urn:issn:0013-9351 https://hdl.handle.net/11250/2763108 https://doi.org/10.1016/j.envres.2020.109906 cristin:1827932 Environmental Research. 2020, 189, 109906 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2020 The Authors |
op_doi |
https://doi.org/10.1016/j.envres.2020.109906 |
container_title |
Environmental Research |
container_volume |
189 |
container_start_page |
109906 |
_version_ |
1766357794159591424 |