Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost
Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old...
Published in: | FEMS Microbiology Ecology |
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Online Access: | https://hdl.handle.net/11250/2757061 https://doi.org/10.1093/femsec/fiaa057 |
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ftunivbergen:oai:bora.uib.no:11250/2757061 2023-05-15T17:55:23+02:00 Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost Xue, Yaxin Jonassen, Inge Øvreås, Lise Tas, Neslihan 2020 application/pdf https://hdl.handle.net/11250/2757061 https://doi.org/10.1093/femsec/fiaa057 eng eng Oxford University Press urn:issn:0168-6496 https://hdl.handle.net/11250/2757061 https://doi.org/10.1093/femsec/fiaa057 cristin:1815842 FEMS Microbiology Ecology. 2020, 96 (5), fiaa057. Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright FEMS 2020 fiaa057 FEMS Microbiology Ecology 96 5 Journal article Peer reviewed 2020 ftunivbergen https://doi.org/10.1093/femsec/fiaa057 2023-03-14T17:43:58Z Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils. publishedVersion Article in Journal/Newspaper permafrost Svalbard University of Bergen: Bergen Open Research Archive (BORA-UiB) Svalbard Norway FEMS Microbiology Ecology 96 5 |
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Open Polar |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
language |
English |
description |
Permafrost underlies a large portion of the land in the Northern Hemisphere. It is proposed to be an extreme habitat and home for cold-adaptive microbial communities. Upon thaw permafrost is predicted to exacerbate increasing global temperature trend, where awakening microbes decompose millennia old carbon stocks. Yet our knowledge on composition, functional potential and variance of permafrost microbiome remains limited. In this study, we conducted a deep comparative metagenomic analysis through a 2 m permafrost core from Svalbard, Norway to determine key permafrost microbiome in this climate sensitive island ecosystem. To do so, we developed comparative metagenomics methods on metagenomic-assembled genomes (MAG). We found that community composition in Svalbard soil horizons shifted markedly with depth: the dominant phylum switched from Acidobacteria and Proteobacteria in top soils (active layer) to Actinobacteria, Bacteroidetes, Chloroflexi and Proteobacteria in permafrost layers. Key metabolic potential propagated through permafrost depths revealed aerobic respiration and soil organic matter decomposition as key metabolic traits. We also found that Svalbard MAGs were enriched in genes involved in regulation of ammonium, sulfur and phosphate. Here, we provide a new perspective on how permafrost microbiome is shaped to acquire resources in competitive and limited resource conditions of deep Svalbard soils. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Tas, Neslihan |
spellingShingle |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Tas, Neslihan Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
author_facet |
Xue, Yaxin Jonassen, Inge Øvreås, Lise Tas, Neslihan |
author_sort |
Xue, Yaxin |
title |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_short |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_full |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_fullStr |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_full_unstemmed |
Metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in Svalbard permafrost |
title_sort |
metagenome-assembled genome distribution and key functionality highlight importance of aerobic metabolism in svalbard permafrost |
publisher |
Oxford University Press |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2757061 https://doi.org/10.1093/femsec/fiaa057 |
geographic |
Svalbard Norway |
geographic_facet |
Svalbard Norway |
genre |
permafrost Svalbard |
genre_facet |
permafrost Svalbard |
op_source |
fiaa057 FEMS Microbiology Ecology 96 5 |
op_relation |
urn:issn:0168-6496 https://hdl.handle.net/11250/2757061 https://doi.org/10.1093/femsec/fiaa057 cristin:1815842 FEMS Microbiology Ecology. 2020, 96 (5), fiaa057. |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright FEMS 2020 |
op_doi |
https://doi.org/10.1093/femsec/fiaa057 |
container_title |
FEMS Microbiology Ecology |
container_volume |
96 |
container_issue |
5 |
_version_ |
1766163309774503936 |