A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts
To avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology...
Published in: | Experimental Eye Research |
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Online Access: | https://hdl.handle.net/11250/2740369 https://doi.org/10.1016/j.exer.2020.108150 |
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ftunivbergen:oai:bora.uib.no:11250/2740369 2023-05-15T15:30:07+02:00 A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune 2020 application/pdf https://hdl.handle.net/11250/2740369 https://doi.org/10.1016/j.exer.2020.108150 eng eng Elsevier urn:issn:0014-4835 https://hdl.handle.net/11250/2740369 https://doi.org/10.1016/j.exer.2020.108150 cristin:1847035 Experimental Eye Research. 2020, 199:108150 Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2020 The Authors 108150 Experimental Eye Research 199:108150 199 Journal article Peer reviewed 2020 ftunivbergen https://doi.org/10.1016/j.exer.2020.108150 2023-03-14T17:41:32Z To avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology compared to diploid fish, with increased prevalence of vertebral deformities and ocular cataracts as two of the most severe disorders. Here, we investigated the mechanisms behind the higher prevalence of cataracts in triploid salmon, by comparing the transcriptional patterns in lenses of diploid and triploid Atlantic salmon, with and without cataracts. We assembled and characterized the Atlantic salmon lens transcriptome and used RNA-seq to search for the molecular basis for cataract development in triploid fish. Transcriptional screening showed only modest differences in lens mRNA levels in diploid and triploid fish, with few uniquely expressed genes. In total, there were 165 differentially expressed genes (DEGs) between the cataractous diploid and triploid lens. Of these, most were expressed at lower levels in triploid fish. Differential expression was observed for genes encoding proteins with known function in the retina (phototransduction) and proteins associated with repair and compensation mechanisms. The results suggest a higher susceptibility to oxidative stress in triploid lenses, and that mechanisms connected to the ability to handle damaged proteins are differentially affected in cataractous lenses from diploid and triploid salmon. publishedVersion Article in Journal/Newspaper Atlantic salmon University of Bergen: Bergen Open Research Archive (BORA-UiB) Experimental Eye Research 199 108150 |
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Open Polar |
collection |
University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
language |
English |
description |
To avoid negative environmental impacts of escapees and potential inter-breeding with wild populations, the Atlantic salmon farming industry has and continues to extensively test triploid fish that are sterile. However, they often show differences in performance, physiology, behavior and morphology compared to diploid fish, with increased prevalence of vertebral deformities and ocular cataracts as two of the most severe disorders. Here, we investigated the mechanisms behind the higher prevalence of cataracts in triploid salmon, by comparing the transcriptional patterns in lenses of diploid and triploid Atlantic salmon, with and without cataracts. We assembled and characterized the Atlantic salmon lens transcriptome and used RNA-seq to search for the molecular basis for cataract development in triploid fish. Transcriptional screening showed only modest differences in lens mRNA levels in diploid and triploid fish, with few uniquely expressed genes. In total, there were 165 differentially expressed genes (DEGs) between the cataractous diploid and triploid lens. Of these, most were expressed at lower levels in triploid fish. Differential expression was observed for genes encoding proteins with known function in the retina (phototransduction) and proteins associated with repair and compensation mechanisms. The results suggest a higher susceptibility to oxidative stress in triploid lenses, and that mechanisms connected to the ability to handle damaged proteins are differentially affected in cataractous lenses from diploid and triploid salmon. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune |
spellingShingle |
Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
author_facet |
Olsvik, Pål Asgeir Finn, Roderick Nigel Remø, Sofie C. Fjelldal, Per Gunnar Chauvigné, Francois Glover, Kevin Hansen, Tom Johnny Waagbø, Rune |
author_sort |
Olsvik, Pål Asgeir |
title |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_short |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_full |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_fullStr |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_full_unstemmed |
A transcriptomic analysis of diploid and triploid Atlantic salmon lenses with and without cataracts |
title_sort |
transcriptomic analysis of diploid and triploid atlantic salmon lenses with and without cataracts |
publisher |
Elsevier |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2740369 https://doi.org/10.1016/j.exer.2020.108150 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
108150 Experimental Eye Research 199:108150 199 |
op_relation |
urn:issn:0014-4835 https://hdl.handle.net/11250/2740369 https://doi.org/10.1016/j.exer.2020.108150 cristin:1847035 Experimental Eye Research. 2020, 199:108150 |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright 2020 The Authors |
op_doi |
https://doi.org/10.1016/j.exer.2020.108150 |
container_title |
Experimental Eye Research |
container_volume |
199 |
container_start_page |
108150 |
_version_ |
1766360572333391872 |