Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines
Background In several fish species homozygous and heterozygous clonal lines have been produced using gynogenetic and androgenetic techniques. These lines are standardized and can be reproduced over generations. In rainbow trout such lines have existed for decades and has become important research to...
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ftunivbergen:oai:bora.uib.no:11250/2735332 2023-05-15T15:28:19+02:00 Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines Hansen, Tom Johnny Penman, David J. Glover, Kevin Fraser, Thomas Vågseth, Tone Thorsen, Anders Sørvik, Anne Grete Eide Fjelldal, Per Gunnar 2020 application/pdf https://hdl.handle.net/11250/2735332 https://doi.org/10.1186/s12863-020-00878-8 eng eng BMC urn:issn:1471-2156 https://hdl.handle.net/11250/2735332 https://doi.org/10.1186/s12863-020-00878-8 cristin:1820452 BMC Genetics. 2020, 21, 71. Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright The Author(s). 2020 71 BMC Genetics 21 Journal article Peer reviewed 2020 ftunivbergen https://doi.org/10.1186/s12863-020-00878-8 2023-03-14T17:41:05Z Background In several fish species homozygous and heterozygous clonal lines have been produced using gynogenetic and androgenetic techniques. These lines are standardized and can be reproduced over generations. In rainbow trout such lines have existed for decades and has become important research tools in genome studies as well as in studies of commercially important traits. The Atlantic salmon is one of the best studied fish species globally, but all experiments are done on fish of wild or domesticated origin and access to standardized immortal fish lines would be of great benefit. Here, we describe the protocols developed to produce mitotic gynogenes, and from these the first clonal lines in Atlantic salmon. Results Atlantic salmon eggs fertilized with UV irradiated sperm combined with a pressure shock applied at 4700–4800 minC at 8 °C gave all homozygous (doubled haploid) gynogenetic progeny with high survival. From the six first maturing females, five all homozygous clonal lines were produced by meiotic gynogenesis and were verified as clonal and identical to their mother with microsatellite markers. Conclusions We have now produced the first documented cloned Atlantic salmon lines. This work demonstrates the potential for production of further Atlantic salmon clonal lines, potentially with distinct characteristics. Such lines will provide an important resource for further elucidation of phenotypic and genetic traits in this globally important species. publishedVersion Article in Journal/Newspaper Atlantic salmon Salmo salar University of Bergen: Bergen Open Research Archive (BORA-UiB) BMC Genetics 21 1 |
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University of Bergen: Bergen Open Research Archive (BORA-UiB) |
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ftunivbergen |
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English |
description |
Background In several fish species homozygous and heterozygous clonal lines have been produced using gynogenetic and androgenetic techniques. These lines are standardized and can be reproduced over generations. In rainbow trout such lines have existed for decades and has become important research tools in genome studies as well as in studies of commercially important traits. The Atlantic salmon is one of the best studied fish species globally, but all experiments are done on fish of wild or domesticated origin and access to standardized immortal fish lines would be of great benefit. Here, we describe the protocols developed to produce mitotic gynogenes, and from these the first clonal lines in Atlantic salmon. Results Atlantic salmon eggs fertilized with UV irradiated sperm combined with a pressure shock applied at 4700–4800 minC at 8 °C gave all homozygous (doubled haploid) gynogenetic progeny with high survival. From the six first maturing females, five all homozygous clonal lines were produced by meiotic gynogenesis and were verified as clonal and identical to their mother with microsatellite markers. Conclusions We have now produced the first documented cloned Atlantic salmon lines. This work demonstrates the potential for production of further Atlantic salmon clonal lines, potentially with distinct characteristics. Such lines will provide an important resource for further elucidation of phenotypic and genetic traits in this globally important species. publishedVersion |
format |
Article in Journal/Newspaper |
author |
Hansen, Tom Johnny Penman, David J. Glover, Kevin Fraser, Thomas Vågseth, Tone Thorsen, Anders Sørvik, Anne Grete Eide Fjelldal, Per Gunnar |
spellingShingle |
Hansen, Tom Johnny Penman, David J. Glover, Kevin Fraser, Thomas Vågseth, Tone Thorsen, Anders Sørvik, Anne Grete Eide Fjelldal, Per Gunnar Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines |
author_facet |
Hansen, Tom Johnny Penman, David J. Glover, Kevin Fraser, Thomas Vågseth, Tone Thorsen, Anders Sørvik, Anne Grete Eide Fjelldal, Per Gunnar |
author_sort |
Hansen, Tom Johnny |
title |
Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines |
title_short |
Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines |
title_full |
Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines |
title_fullStr |
Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines |
title_full_unstemmed |
Production and verification of the first Atlantic salmon (Salmo salar L.) clonal lines |
title_sort |
production and verification of the first atlantic salmon (salmo salar l.) clonal lines |
publisher |
BMC |
publishDate |
2020 |
url |
https://hdl.handle.net/11250/2735332 https://doi.org/10.1186/s12863-020-00878-8 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
71 BMC Genetics 21 |
op_relation |
urn:issn:1471-2156 https://hdl.handle.net/11250/2735332 https://doi.org/10.1186/s12863-020-00878-8 cristin:1820452 BMC Genetics. 2020, 21, 71. |
op_rights |
Navngivelse 4.0 Internasjonal http://creativecommons.org/licenses/by/4.0/deed.no Copyright The Author(s). 2020 |
op_doi |
https://doi.org/10.1186/s12863-020-00878-8 |
container_title |
BMC Genetics |
container_volume |
21 |
container_issue |
1 |
_version_ |
1766358694504693760 |