Detection and genotyping of Toxoplasma gondii in wild canids in Serbia
To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus)...
Published in: | Parasitology International |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
Elsevier Ireland Ltd, Clare
2019
|
Subjects: | |
Online Access: | http://rimi.imi.bg.ac.rs/handle/123456789/906 https://doi.org/10.1016/j.parint.2019.101973 |
id |
ftunivbelgradimi:oai:rimi.imi.bg.ac.rs:123456789/906 |
---|---|
record_format |
openpolar |
spelling |
ftunivbelgradimi:oai:rimi.imi.bg.ac.rs:123456789/906 2024-05-12T08:02:15+00:00 Detection and genotyping of Toxoplasma gondii in wild canids in Serbia Uzelac, Aleksandra Klun, Ivana Ćirović, Duško Penezić, Aleksandra Ćirković, Vladimir Đurković-Đaković, Olgica 2019 http://rimi.imi.bg.ac.rs/handle/123456789/906 https://doi.org/10.1016/j.parint.2019.101973 unknown Elsevier Ireland Ltd, Clare info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41019/RS// info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31009/RS// 1383-5769 http://rimi.imi.bg.ac.rs/handle/123456789/906 doi:10.1016/j.parint.2019.101973 31404655 2-s2.0-85070808415 000487571300006 restrictedAccess ARR Parasitology International Toxoplasma gondii Genotyping Strains Golden jackal Red fox Grey wolf article publishedVersion 2019 ftunivbelgradimi https://doi.org/10.1016/j.parint.2019.101973 2024-04-15T16:55:04Z To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%) were positive for T. gondii DNA, of which 24 were genotyped (nine red foxes, 13 golden jackals and two wolves). The results showed a large dominance of lineage II (n = 20, 83.4%) over lineage III (n = 4, 16.7%) strains. Lineage II comprised four archetypes, 14 variants and one which remained undetermined, while within lineage III there were three archetypes and one variant strain. However, a notable difference in diversity was observed among the examined canids, with eight (88.9%) of the nine strains detected in red foxes distinct, vs. six (46.1%) distinct strains of the 13 detected in golden jackals. Since anthropogenic food sources are a major reservoir of archetypal lineage II and III T. gondii strains, the results suggest that the golden jackal may be outcompeting the red fox, forcing it to hunt wild mammals and birds for sustenance, thereby increasing its chances of infection with variant strains. In addition to presenting the first data on the T. gondii population structure in wild canids in Serbia, this is the first report ever on T. gondii genotypes in golden jackals, a species with an increasing presence in Europe and another important reservoir of domestically circulating T. gondii strains. Article in Journal/Newspaper Canis lupus RIMI - Repository of the Institute for Medical Research Parasitology International 73 101973 |
institution |
Open Polar |
collection |
RIMI - Repository of the Institute for Medical Research |
op_collection_id |
ftunivbelgradimi |
language |
unknown |
topic |
Toxoplasma gondii Genotyping Strains Golden jackal Red fox Grey wolf |
spellingShingle |
Toxoplasma gondii Genotyping Strains Golden jackal Red fox Grey wolf Uzelac, Aleksandra Klun, Ivana Ćirović, Duško Penezić, Aleksandra Ćirković, Vladimir Đurković-Đaković, Olgica Detection and genotyping of Toxoplasma gondii in wild canids in Serbia |
topic_facet |
Toxoplasma gondii Genotyping Strains Golden jackal Red fox Grey wolf |
description |
To gain insight into the population structure of the protozoan parasite Toxoplasma gondii in wildlife in Serbia, multiplex nested PCR- RFLP was performed on T. gondii DNA positive samples of heart tissue of three wild canid species, including red foxes (Vulpes vulpes), golden jackals (Canis aureus) and grey wolves (Canis lupus). Out of 110 samples (28 red foxes, 72 golden jackals, 10 wolves), 30 (27.3%) were positive for T. gondii DNA, of which 24 were genotyped (nine red foxes, 13 golden jackals and two wolves). The results showed a large dominance of lineage II (n = 20, 83.4%) over lineage III (n = 4, 16.7%) strains. Lineage II comprised four archetypes, 14 variants and one which remained undetermined, while within lineage III there were three archetypes and one variant strain. However, a notable difference in diversity was observed among the examined canids, with eight (88.9%) of the nine strains detected in red foxes distinct, vs. six (46.1%) distinct strains of the 13 detected in golden jackals. Since anthropogenic food sources are a major reservoir of archetypal lineage II and III T. gondii strains, the results suggest that the golden jackal may be outcompeting the red fox, forcing it to hunt wild mammals and birds for sustenance, thereby increasing its chances of infection with variant strains. In addition to presenting the first data on the T. gondii population structure in wild canids in Serbia, this is the first report ever on T. gondii genotypes in golden jackals, a species with an increasing presence in Europe and another important reservoir of domestically circulating T. gondii strains. |
format |
Article in Journal/Newspaper |
author |
Uzelac, Aleksandra Klun, Ivana Ćirović, Duško Penezić, Aleksandra Ćirković, Vladimir Đurković-Đaković, Olgica |
author_facet |
Uzelac, Aleksandra Klun, Ivana Ćirović, Duško Penezić, Aleksandra Ćirković, Vladimir Đurković-Đaković, Olgica |
author_sort |
Uzelac, Aleksandra |
title |
Detection and genotyping of Toxoplasma gondii in wild canids in Serbia |
title_short |
Detection and genotyping of Toxoplasma gondii in wild canids in Serbia |
title_full |
Detection and genotyping of Toxoplasma gondii in wild canids in Serbia |
title_fullStr |
Detection and genotyping of Toxoplasma gondii in wild canids in Serbia |
title_full_unstemmed |
Detection and genotyping of Toxoplasma gondii in wild canids in Serbia |
title_sort |
detection and genotyping of toxoplasma gondii in wild canids in serbia |
publisher |
Elsevier Ireland Ltd, Clare |
publishDate |
2019 |
url |
http://rimi.imi.bg.ac.rs/handle/123456789/906 https://doi.org/10.1016/j.parint.2019.101973 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Parasitology International |
op_relation |
info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/41019/RS// info:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31009/RS// 1383-5769 http://rimi.imi.bg.ac.rs/handle/123456789/906 doi:10.1016/j.parint.2019.101973 31404655 2-s2.0-85070808415 000487571300006 |
op_rights |
restrictedAccess ARR |
op_doi |
https://doi.org/10.1016/j.parint.2019.101973 |
container_title |
Parasitology International |
container_volume |
73 |
container_start_page |
101973 |
_version_ |
1798844346055786496 |