Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment
Immobilization of lipase B from Candida antarctica was carried out using three different immobilization methods, and two epoxy activated supports, comercial Eupergit C and synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The synthesized pol...
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ftunivbelgradftm:oai:TechnoRep.tmf.bg.ac.rs:123456789/6096 2023-12-24T10:11:24+01:00 Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment Jakovetić, Sonja Picazo-Espinosa, Rafael Manzanera, Maximino Stojanović, Željko Prodanović, Radivoje Miladinović, Ružica Knežević-Jugović, Zorica 2012 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6096 https://hdl.handle.net/21.15107/rcub_technorep_6096 en eng info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// 978-86-7132-049-8 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6096 https://hdl.handle.net/21.15107/rcub_technorep_6096 restrictedAccess ARR conferenceObject publishedVersion 2012 ftunivbelgradftm 2023-11-28T17:19:59Z Immobilization of lipase B from Candida antarctica was carried out using three different immobilization methods, and two epoxy activated supports, comercial Eupergit C and synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of the effect of immobilization pH on the activity of immobilized enzymes, showed that the highest activities were obtained when immobilization process was conducted in acidic enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was achieved when periodate method was employed. Glutaraldehyde activation of support did not improve activity of obtained immobilized enzymes. Conference Object Antarc* Antarctica TechnoRep - Faculty of Technology and Metallurgy Repository |
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Open Polar |
collection |
TechnoRep - Faculty of Technology and Metallurgy Repository |
op_collection_id |
ftunivbelgradftm |
language |
English |
description |
Immobilization of lipase B from Candida antarctica was carried out using three different immobilization methods, and two epoxy activated supports, comercial Eupergit C and synthesized macroporous copolymer of glycidyl methacrylate (GMA) and ethylene glycol dimethacrylate (EGDMA). The synthesized polymer was proven to be better for CALB immobilization at both ionic strenghts that wrere applied, 0.1M and 1.25M. Investigation of the effect of immobilization pH on the activity of immobilized enzymes, showed that the highest activities were obtained when immobilization process was conducted in acidic enviorments. The highest immobilized enzyme activity, 30.5 IU/ g of dry support, was achieved when periodate method was employed. Glutaraldehyde activation of support did not improve activity of obtained immobilized enzymes. |
format |
Conference Object |
author |
Jakovetić, Sonja Picazo-Espinosa, Rafael Manzanera, Maximino Stojanović, Željko Prodanović, Radivoje Miladinović, Ružica Knežević-Jugović, Zorica |
spellingShingle |
Jakovetić, Sonja Picazo-Espinosa, Rafael Manzanera, Maximino Stojanović, Željko Prodanović, Radivoje Miladinović, Ružica Knežević-Jugović, Zorica Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment |
author_facet |
Jakovetić, Sonja Picazo-Espinosa, Rafael Manzanera, Maximino Stojanović, Željko Prodanović, Radivoje Miladinović, Ružica Knežević-Jugović, Zorica |
author_sort |
Jakovetić, Sonja |
title |
Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment |
title_short |
Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment |
title_full |
Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment |
title_fullStr |
Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment |
title_full_unstemmed |
Immobilization of Candida antarctica lipase B on supports with epoxy groups via covalent attachment |
title_sort |
immobilization of candida antarctica lipase b on supports with epoxy groups via covalent attachment |
publishDate |
2012 |
url |
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6096 https://hdl.handle.net/21.15107/rcub_technorep_6096 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// 978-86-7132-049-8 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/6096 https://hdl.handle.net/21.15107/rcub_technorep_6096 |
op_rights |
restrictedAccess ARR |
_version_ |
1786164311918379008 |