Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102
L-Ascorbyl oleate is recognized as efficient liposoluble antioxidant and biosurfactant which can be synthesized by using immobilized lipases as catalysts in organic media. However, development of economical production process is still a challenge, particularly in (semi)continuous systems. Hereby, we...
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Elsevier, Amsterdam
2017
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ftunivbelgradftm:oai:TechnoRep.tmf.bg.ac.rs:123456789/3650 2024-02-11T09:56:56+01:00 Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 Ćorović, Marija Milivojević, Ana Carević, Milica Banjanac, Katarina Jakovetić Tanasković, Sonja Bezbradica, Dejan 2017 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3650 https://doi.org/10.1016/j.cherd.2017.08.021 unknown Elsevier, Amsterdam info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// 0263-8762 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3650 doi:10.1016/j.cherd.2017.08.021 2-s2.0-85031115544 000413387600015 restrictedAccess ARR Chemical Engineering Research & Design Immobilized lipase Ascorbyl oleate Esterification Fluidized bed bioreactor Kinetics Hydrodynamics article publishedVersion 2017 ftunivbelgradftm https://doi.org/10.1016/j.cherd.2017.08.021 2024-01-16T17:19:47Z L-Ascorbyl oleate is recognized as efficient liposoluble antioxidant and biosurfactant which can be synthesized by using immobilized lipases as catalysts in organic media. However, development of economical production process is still a challenge, particularly in (semi)continuous systems. Hereby, we present application of novel immobilized lipase preparation, lipase B from Candida antarctica hydrophobically adsorbed onto styrene divinylbenzene based support, for the synthesis of L-ascorbyl oleate in batch bioreactor (BB) and fluidized bed bioreactor (FBB) with recirculation of reaction medium. Reaction kinetics was described by Ping Pong bi bi mechanism with low acyl donor inhibition. Considerably higher reaction rates (similar to 40%) were accomplished in FBB. Hydrodynamic study revealed that reaction is kinetically controlled and unhindered external and internal mass transfer was enabled in both systems. However, much higher shear stress occurred in BB (10.46 Pa) comparing to FBB (5 x 10(-2) Pa). After 12 consecutive reaction cycles lasting 24h, 23.13 g of product per gram of biocatalyst was synthesized in a FBB. Within this study, usage of new immobilized enzyme in semicontinuous system enabled high reaction rates and satisfying operational stability in the synthesis of L-ascorbyl oleate, offering good prospect for further process development. Article in Journal/Newspaper Antarc* Antarctica TechnoRep - Faculty of Technology and Metallurgy Repository Chemical Engineering Research and Design 126 161 171 |
institution |
Open Polar |
collection |
TechnoRep - Faculty of Technology and Metallurgy Repository |
op_collection_id |
ftunivbelgradftm |
language |
unknown |
topic |
Immobilized lipase Ascorbyl oleate Esterification Fluidized bed bioreactor Kinetics Hydrodynamics |
spellingShingle |
Immobilized lipase Ascorbyl oleate Esterification Fluidized bed bioreactor Kinetics Hydrodynamics Ćorović, Marija Milivojević, Ana Carević, Milica Banjanac, Katarina Jakovetić Tanasković, Sonja Bezbradica, Dejan Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 |
topic_facet |
Immobilized lipase Ascorbyl oleate Esterification Fluidized bed bioreactor Kinetics Hydrodynamics |
description |
L-Ascorbyl oleate is recognized as efficient liposoluble antioxidant and biosurfactant which can be synthesized by using immobilized lipases as catalysts in organic media. However, development of economical production process is still a challenge, particularly in (semi)continuous systems. Hereby, we present application of novel immobilized lipase preparation, lipase B from Candida antarctica hydrophobically adsorbed onto styrene divinylbenzene based support, for the synthesis of L-ascorbyl oleate in batch bioreactor (BB) and fluidized bed bioreactor (FBB) with recirculation of reaction medium. Reaction kinetics was described by Ping Pong bi bi mechanism with low acyl donor inhibition. Considerably higher reaction rates (similar to 40%) were accomplished in FBB. Hydrodynamic study revealed that reaction is kinetically controlled and unhindered external and internal mass transfer was enabled in both systems. However, much higher shear stress occurred in BB (10.46 Pa) comparing to FBB (5 x 10(-2) Pa). After 12 consecutive reaction cycles lasting 24h, 23.13 g of product per gram of biocatalyst was synthesized in a FBB. Within this study, usage of new immobilized enzyme in semicontinuous system enabled high reaction rates and satisfying operational stability in the synthesis of L-ascorbyl oleate, offering good prospect for further process development. |
format |
Article in Journal/Newspaper |
author |
Ćorović, Marija Milivojević, Ana Carević, Milica Banjanac, Katarina Jakovetić Tanasković, Sonja Bezbradica, Dejan |
author_facet |
Ćorović, Marija Milivojević, Ana Carević, Milica Banjanac, Katarina Jakovetić Tanasković, Sonja Bezbradica, Dejan |
author_sort |
Ćorović, Marija |
title |
Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 |
title_short |
Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 |
title_full |
Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 |
title_fullStr |
Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 |
title_full_unstemmed |
Batch and semicontinuous production of L-ascorbyl oleate catalyzed by CALB immobilized onto Purolite (R) MN102 |
title_sort |
batch and semicontinuous production of l-ascorbyl oleate catalyzed by calb immobilized onto purolite (r) mn102 |
publisher |
Elsevier, Amsterdam |
publishDate |
2017 |
url |
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3650 https://doi.org/10.1016/j.cherd.2017.08.021 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Chemical Engineering Research & Design |
op_relation |
info:eu-repo/grantAgreement/MESTD/Integrated and Interdisciplinary Research (IIR or III)/46010/RS// 0263-8762 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/3650 doi:10.1016/j.cherd.2017.08.021 2-s2.0-85031115544 000413387600015 |
op_rights |
restrictedAccess ARR |
op_doi |
https://doi.org/10.1016/j.cherd.2017.08.021 |
container_title |
Chemical Engineering Research and Design |
container_volume |
126 |
container_start_page |
161 |
op_container_end_page |
171 |
_version_ |
1790607061923397632 |