Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica
Cinnamic acid and its esters are widespread throughout plant kingdom and therefore, they are common components of our daily diet. Ethyl cinnamate has been approved by FDA for direct addition to food for human consumption. Enzyme synthesis is strongly preferred compared to chemically catalyzed esters...
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6th Central European Congress on Food, CEFood 2012
2012
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ftunivbelgradftm:oai:TechnoRep.tmf.bg.ac.rs:123456789/1973 2023-12-24T10:10:10+01:00 Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica Jakovetić, Sonja Knežević-Jugović, Zorica Jugović, Branimir Gvozdenović, Milica Bezbradica, Dejan 2012 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1973 https://hdl.handle.net/21.15107/rcub_technorep_1973 unknown 6th Central European Congress on Food, CEFood 2012 978-867994027-8 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1973 https://hdl.handle.net/21.15107/rcub_technorep_1973 2-s2.0-84889260718 restrictedAccess ARR CEFood 2012 - Proceedings of 6th Central European Congress on Food Antioxidant activity Cinnamic acid Cyclic voltammetry Enzymatic esterification Ethyl cinnamate Radical-scavenging activity conferenceObject publishedVersion 2012 ftunivbelgradftm 2023-11-28T17:19:52Z Cinnamic acid and its esters are widespread throughout plant kingdom and therefore, they are common components of our daily diet. Ethyl cinnamate has been approved by FDA for direct addition to food for human consumption. Enzyme synthesis is strongly preferred compared to chemically catalyzed esters synthesis when product quality is a main issue, as is the case for food production. The aim of this study was to examine possibilities for enzyme catalyzed synthesis of cinnamic acid esters and to optimize the synthesis of ethyl cinnamate in terms of selected parameters, including the type of the organic solvent and substrate initial molar ratio. All reactions were performed batchwise under pH and temperature control in vessels containing 5 mL of reaction medium, using cinnamic acid as limiting substrate (0.167 M). Each reaction mixture was supplemented with 75 mg of commercial immobilized lipase B from Candida antarctica, Novozyme 435. The antioxidant activity of obtained ester was measured by using chemical and electrochemical techniques. We have proven that ethyl cinnamate can be synthesized using lipase B from Candida antarctica with very high reaction yields in the simple bioreactor system.The best reaction yield (89%) was obtained in isooctane when substrate molar ratio of 1:3 was used. Both, DPPH assay and cyclic voltammetry measurement has shown that ethyl cinnamate has better antioxidant properties than cinnamic acid itself. Conference Object Antarc* Antarctica TechnoRep - Faculty of Technology and Metallurgy Repository |
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TechnoRep - Faculty of Technology and Metallurgy Repository |
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topic |
Antioxidant activity Cinnamic acid Cyclic voltammetry Enzymatic esterification Ethyl cinnamate Radical-scavenging activity |
spellingShingle |
Antioxidant activity Cinnamic acid Cyclic voltammetry Enzymatic esterification Ethyl cinnamate Radical-scavenging activity Jakovetić, Sonja Knežević-Jugović, Zorica Jugović, Branimir Gvozdenović, Milica Bezbradica, Dejan Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica |
topic_facet |
Antioxidant activity Cinnamic acid Cyclic voltammetry Enzymatic esterification Ethyl cinnamate Radical-scavenging activity |
description |
Cinnamic acid and its esters are widespread throughout plant kingdom and therefore, they are common components of our daily diet. Ethyl cinnamate has been approved by FDA for direct addition to food for human consumption. Enzyme synthesis is strongly preferred compared to chemically catalyzed esters synthesis when product quality is a main issue, as is the case for food production. The aim of this study was to examine possibilities for enzyme catalyzed synthesis of cinnamic acid esters and to optimize the synthesis of ethyl cinnamate in terms of selected parameters, including the type of the organic solvent and substrate initial molar ratio. All reactions were performed batchwise under pH and temperature control in vessels containing 5 mL of reaction medium, using cinnamic acid as limiting substrate (0.167 M). Each reaction mixture was supplemented with 75 mg of commercial immobilized lipase B from Candida antarctica, Novozyme 435. The antioxidant activity of obtained ester was measured by using chemical and electrochemical techniques. We have proven that ethyl cinnamate can be synthesized using lipase B from Candida antarctica with very high reaction yields in the simple bioreactor system.The best reaction yield (89%) was obtained in isooctane when substrate molar ratio of 1:3 was used. Both, DPPH assay and cyclic voltammetry measurement has shown that ethyl cinnamate has better antioxidant properties than cinnamic acid itself. |
format |
Conference Object |
author |
Jakovetić, Sonja Knežević-Jugović, Zorica Jugović, Branimir Gvozdenović, Milica Bezbradica, Dejan |
author_facet |
Jakovetić, Sonja Knežević-Jugović, Zorica Jugović, Branimir Gvozdenović, Milica Bezbradica, Dejan |
author_sort |
Jakovetić, Sonja |
title |
Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica |
title_short |
Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica |
title_full |
Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica |
title_fullStr |
Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica |
title_full_unstemmed |
Synthesis of ethyl cinnamate catalyzed by lipase B from Candida antarctica |
title_sort |
synthesis of ethyl cinnamate catalyzed by lipase b from candida antarctica |
publisher |
6th Central European Congress on Food, CEFood 2012 |
publishDate |
2012 |
url |
http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1973 https://hdl.handle.net/21.15107/rcub_technorep_1973 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
CEFood 2012 - Proceedings of 6th Central European Congress on Food |
op_relation |
978-867994027-8 http://TechnoRep.tmf.bg.ac.rs/handle/123456789/1973 https://hdl.handle.net/21.15107/rcub_technorep_1973 2-s2.0-84889260718 |
op_rights |
restrictedAccess ARR |
_version_ |
1786213757886660608 |