Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil
Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10degreesC and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further charac...
Published in: | Applied and Environmental Microbiology |
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Language: | English |
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2003
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Online Access: | https://researchportal.bath.ac.uk/en/publications/f457f8f9-4596-452a-a879-5fb2ac215cbd https://doi.org/10.1128/aem.69.3.1457-1464.2003 |
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ftunivbathcris:oai:purehost.bath.ac.uk:publications/f457f8f9-4596-452a-a879-5fb2ac215cbd 2024-04-28T08:00:06+00:00 Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. 2003 https://researchportal.bath.ac.uk/en/publications/f457f8f9-4596-452a-a879-5fb2ac215cbd https://doi.org/10.1128/aem.69.3.1457-1464.2003 eng eng https://researchportal.bath.ac.uk/en/publications/f457f8f9-4596-452a-a879-5fb2ac215cbd info:eu-repo/semantics/restrictedAccess Wery , N , Gerike , U , Sharman , A , Chaudhuri , J B , Hough , D W & Danson , M J 2003 , ' Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil ' , Applied and Environmental Microbiology , vol. 69 , no. 3 , pp. 1457-1464 . https://doi.org/10.1128/aem.69.3.1457-1464.2003 article 2003 ftunivbathcris https://doi.org/10.1128/aem.69.3.1457-1464.2003 2024-04-09T02:36:48Z Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10degreesC and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. Phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological and metabolical diversity. The extracellular protease activities tested were optimal at neutral pH and between 30 and 45degreesC. 16S ribosomal DNA sequence analyses showed that the bioreactor was colonized by a wide variety of taxons, belonging to various bacterial divisions: alpha-, beta-, and gamma-Proteobacteria; the Flexibacter-Cytophaga-Bacteroides group; and high G+C gram-positive bacteria and low G+C gram-positive bacteria. Some strains represent candidates for new species of the genera Chryseobacterium and Massilia. This diversity demonstrates that the bioreactor is an efficient enrichment tool compared to traditional isolation strategies. Article in Journal/Newspaper Antarc* Antarctic Antarctica University of Bath's research portal Applied and Environmental Microbiology 69 3 1457 1464 |
institution |
Open Polar |
collection |
University of Bath's research portal |
op_collection_id |
ftunivbathcris |
language |
English |
description |
Seventy-five aerobic heterotrophs have been isolated from a packed-column bioreactor inoculated with soil from Antarctica. The column was maintained at 10degreesC and continuously fed with a casein-containing medium to enrich protease producers. Twenty-eight isolates were selected for further characterization on the basis of morphology and production of clearing zones on skim milk plates. Phenotypic tests indicated that the strains were mainly psychrotrophs and presented a high morphological and metabolical diversity. The extracellular protease activities tested were optimal at neutral pH and between 30 and 45degreesC. 16S ribosomal DNA sequence analyses showed that the bioreactor was colonized by a wide variety of taxons, belonging to various bacterial divisions: alpha-, beta-, and gamma-Proteobacteria; the Flexibacter-Cytophaga-Bacteroides group; and high G+C gram-positive bacteria and low G+C gram-positive bacteria. Some strains represent candidates for new species of the genera Chryseobacterium and Massilia. This diversity demonstrates that the bioreactor is an efficient enrichment tool compared to traditional isolation strategies. |
format |
Article in Journal/Newspaper |
author |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. |
spellingShingle |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
author_facet |
Wery, Nathalie Gerike, Ursula Sharman, Ajay Chaudhuri, Julian B. Hough, David W. Danson, Michael J. |
author_sort |
Wery, Nathalie |
title |
Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
title_short |
Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
title_full |
Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
title_fullStr |
Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
title_full_unstemmed |
Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
title_sort |
use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil |
publishDate |
2003 |
url |
https://researchportal.bath.ac.uk/en/publications/f457f8f9-4596-452a-a879-5fb2ac215cbd https://doi.org/10.1128/aem.69.3.1457-1464.2003 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Wery , N , Gerike , U , Sharman , A , Chaudhuri , J B , Hough , D W & Danson , M J 2003 , ' Use of a packed-column bioreactor for isolation of diverse protease-producing bacteria from antarctic soil ' , Applied and Environmental Microbiology , vol. 69 , no. 3 , pp. 1457-1464 . https://doi.org/10.1128/aem.69.3.1457-1464.2003 |
op_relation |
https://researchportal.bath.ac.uk/en/publications/f457f8f9-4596-452a-a879-5fb2ac215cbd |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1128/aem.69.3.1457-1464.2003 |
container_title |
Applied and Environmental Microbiology |
container_volume |
69 |
container_issue |
3 |
container_start_page |
1457 |
op_container_end_page |
1464 |
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1797572481377107968 |