Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state
The lumpfish, Cyclopterus lumpus, holds significant promise as a candidate for large-scale aquaculture production, particularly in its role as a cleaner fish used to manage sea lice infestations in Atlantic salmon Salmo salar farming. Melanomacrophage centers (MMCs) represent polymorphic structures...
Published in: | Aquaculture |
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2024
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Online Access: | https://hdl.handle.net/11586/459720 https://doi.org/10.1016/j.aquaculture.2023.740417 https://www.sciencedirect.com/science/article/pii/S0044848623011912?pes=vor |
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ftunivbari:oai:ricerca.uniba.it:11586/459720 2024-04-21T07:57:28+00:00 Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state Passantino L. Corriero A. Pousis C. Zupa R. Perillo A. Superio J. Kumari Swain J. Foss A. Galindo-Villegas J. Ventriglia G. Passantino, L. Corriero, A. Pousis, C. Zupa, R. Perillo, A. Superio, J. Kumari Swain, J. Foss, A. Galindo-Villegas, J. Ventriglia, G. 2024 https://hdl.handle.net/11586/459720 https://doi.org/10.1016/j.aquaculture.2023.740417 https://www.sciencedirect.com/science/article/pii/S0044848623011912?pes=vor eng eng volume:581 journal:AQUACULTURE https://hdl.handle.net/11586/459720 doi:10.1016/j.aquaculture.2023.740417 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85178336159 https://www.sciencedirect.com/science/article/pii/S0044848623011912?pes=vor CYP1A Health markers immunity Necrosi Non-model vertebrate info:eu-repo/semantics/article 2024 ftunivbari https://doi.org/10.1016/j.aquaculture.2023.740417 2024-03-28T01:22:48Z The lumpfish, Cyclopterus lumpus, holds significant promise as a candidate for large-scale aquaculture production, particularly in its role as a cleaner fish used to manage sea lice infestations in Atlantic salmon Salmo salar farming. Melanomacrophage centers (MMCs) represent polymorphic structures present in the hemolymphopoietic organs of various vertebrates, serving as a widely applicable histological indicator of the fish immune and health status. This study aims to investigate the histochemical characteristics of MMCs within lumpfish livers and to compare MMC density between hatchery-produced (farmed) and wild individuals. Liver samples were collected from 34 lumpfish and subjected to a range of staining techniques, including haematoxylin-eosin, Azan-Mallory's trichrome, Masson-Fontana, Perls-Van Geison, Mallory's hemofuscin, immunohistochemical detection of cytochrome P450 monooxygenase 1 A (CYP1A), and the terminal deoxynucleotidyl transferase-mediated d'UTP nick-end labelling (TUNEL) method. Hepatocytes from hatchery-produced males exhibited notably high lipid content. Additionally, cells showing positive staining with Masson-Fontana, likely associated with the monocyte/macrophage lineage, were identified. Furthermore, small MMCs containing melanin, lipofuscin-ceroids, and ferric ions were detected. While the density of single monocytes/macrophages was markedly higher in hatchery-produced males, no significant discrepancies in MMCs density were observed between wild and hatchery-produced fish, or between males and females of the same origin. The study also revealed the presence of necrotic foci, characterized by hypertrophic hepatocytes positive for both TUNEL and CYP1A staining. These hypertrophic hepatocytes displayed large lipid droplets and pycnotic nuclei, with hatchery-produced males showing a higher numerical density of such foci. In contrast to findings in other fish species, the study found that MMCs did not appear to serve as reliable markers of health status in lumpfish. This conclusion was ... Article in Journal/Newspaper Atlantic salmon Salmo salar Università degli Studi di Bari Aldo Moro: CINECA IRIS Aquaculture 581 740417 |
institution |
Open Polar |
collection |
Università degli Studi di Bari Aldo Moro: CINECA IRIS |
op_collection_id |
ftunivbari |
language |
English |
topic |
CYP1A Health markers immunity Necrosi Non-model vertebrate |
spellingShingle |
CYP1A Health markers immunity Necrosi Non-model vertebrate Passantino L. Corriero A. Pousis C. Zupa R. Perillo A. Superio J. Kumari Swain J. Foss A. Galindo-Villegas J. Ventriglia G. Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state |
topic_facet |
CYP1A Health markers immunity Necrosi Non-model vertebrate |
description |
The lumpfish, Cyclopterus lumpus, holds significant promise as a candidate for large-scale aquaculture production, particularly in its role as a cleaner fish used to manage sea lice infestations in Atlantic salmon Salmo salar farming. Melanomacrophage centers (MMCs) represent polymorphic structures present in the hemolymphopoietic organs of various vertebrates, serving as a widely applicable histological indicator of the fish immune and health status. This study aims to investigate the histochemical characteristics of MMCs within lumpfish livers and to compare MMC density between hatchery-produced (farmed) and wild individuals. Liver samples were collected from 34 lumpfish and subjected to a range of staining techniques, including haematoxylin-eosin, Azan-Mallory's trichrome, Masson-Fontana, Perls-Van Geison, Mallory's hemofuscin, immunohistochemical detection of cytochrome P450 monooxygenase 1 A (CYP1A), and the terminal deoxynucleotidyl transferase-mediated d'UTP nick-end labelling (TUNEL) method. Hepatocytes from hatchery-produced males exhibited notably high lipid content. Additionally, cells showing positive staining with Masson-Fontana, likely associated with the monocyte/macrophage lineage, were identified. Furthermore, small MMCs containing melanin, lipofuscin-ceroids, and ferric ions were detected. While the density of single monocytes/macrophages was markedly higher in hatchery-produced males, no significant discrepancies in MMCs density were observed between wild and hatchery-produced fish, or between males and females of the same origin. The study also revealed the presence of necrotic foci, characterized by hypertrophic hepatocytes positive for both TUNEL and CYP1A staining. These hypertrophic hepatocytes displayed large lipid droplets and pycnotic nuclei, with hatchery-produced males showing a higher numerical density of such foci. In contrast to findings in other fish species, the study found that MMCs did not appear to serve as reliable markers of health status in lumpfish. This conclusion was ... |
author2 |
Passantino, L. Corriero, A. Pousis, C. Zupa, R. Perillo, A. Superio, J. Kumari Swain, J. Foss, A. Galindo-Villegas, J. Ventriglia, G. |
format |
Article in Journal/Newspaper |
author |
Passantino L. Corriero A. Pousis C. Zupa R. Perillo A. Superio J. Kumari Swain J. Foss A. Galindo-Villegas J. Ventriglia G. |
author_facet |
Passantino L. Corriero A. Pousis C. Zupa R. Perillo A. Superio J. Kumari Swain J. Foss A. Galindo-Villegas J. Ventriglia G. |
author_sort |
Passantino L. |
title |
Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state |
title_short |
Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state |
title_full |
Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state |
title_fullStr |
Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state |
title_full_unstemmed |
Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state |
title_sort |
hepatic melanomacrophage centers in the arctic cultured fish cyclopterus lumpus are not indicative of its health state |
publishDate |
2024 |
url |
https://hdl.handle.net/11586/459720 https://doi.org/10.1016/j.aquaculture.2023.740417 https://www.sciencedirect.com/science/article/pii/S0044848623011912?pes=vor |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
volume:581 journal:AQUACULTURE https://hdl.handle.net/11586/459720 doi:10.1016/j.aquaculture.2023.740417 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85178336159 https://www.sciencedirect.com/science/article/pii/S0044848623011912?pes=vor |
op_doi |
https://doi.org/10.1016/j.aquaculture.2023.740417 |
container_title |
Aquaculture |
container_volume |
581 |
container_start_page |
740417 |
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1796939091519995904 |