New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis
Here is an updated report on the genomic organization of T cell receptor beta (TRB) locus in the domestic dog (Canis lupus familiaris) as inferred from comparative genomics and expression analysis. The most interesting results we found were a second TRBD–J–C cluster, which is absent from the referen...
Published in: | Developmental & Comparative Immunology |
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ftunivbari:oai:ricerca.uniba.it:11586/126658 2023-12-03T10:20:52+01:00 New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis Mineccia, M Massari, S. Linguiti, G. CECI, Luigi CICCARESE, Salvatrice Maria ANTONACCI, Rachele Mineccia, M Massari, S. Linguiti, G. Ceci, Luigi Ciccarese, Salvatrice Maria Antonacci, Rachele 2012 http://hdl.handle.net/11586/126658 https://doi.org/10.1016/j.dci.2012.03.010 unknown info:eu-repo/semantics/altIdentifier/pmid/22465586 info:eu-repo/semantics/altIdentifier/wos/WOS:000306891400007 volume:37 issue:2 firstpage:279 lastpage:293 numberofpages:15 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11586/126658 doi:10.1016/j.dci.2012.03.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84862146557 info:eu-repo/semantics/openAccess info:eu-repo/semantics/article 2012 ftunivbari https://doi.org/10.1016/j.dci.2012.03.010 2023-11-08T17:51:01Z Here is an updated report on the genomic organization of T cell receptor beta (TRB) locus in the domestic dog (Canis lupus familiaris) as inferred from comparative genomics and expression analysis. The most interesting results we found were a second TRBD–J–C cluster, which is absent from the reference genome sequence, and the annotation of two additional TRBV genes. In dogs, TRB locus consists of a library of 37 TRBV genes positioned at the 50 end of two in tandem aligned D–J–C gene clusters, each composed of a single TRBD, 6 TRBJ and one TRBC genes, followed by a single TRBV gene with an inverted transcriptional orientation. The TRB genes are distributed in less than 300 kb, making the canine locus, one of the smaller mammalian TRB locus studied so far. The small size may be ascribed to reduced gene duplication occurrences and a lower density of total interspersed repeats compared to humans and mice. Despite the low TRBV gene content, a large and diversified beta chain repertoire is displayed in the dog peripheral blood. A full usage of TRBV and TRBJ genes, including pseudogenes, and a high level of allelic polymorphism contribute to generate diversity. Finally, this study suggests that the overall TRB locus organization is evolutionarily conserved supporting the dog as a highly suited model system for immune development and diseases. Article in Journal/Newspaper Canis lupus Università degli Studi di Bari Aldo Moro: CINECA IRIS Developmental & Comparative Immunology 37 2 279 293 |
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Università degli Studi di Bari Aldo Moro: CINECA IRIS |
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Here is an updated report on the genomic organization of T cell receptor beta (TRB) locus in the domestic dog (Canis lupus familiaris) as inferred from comparative genomics and expression analysis. The most interesting results we found were a second TRBD–J–C cluster, which is absent from the reference genome sequence, and the annotation of two additional TRBV genes. In dogs, TRB locus consists of a library of 37 TRBV genes positioned at the 50 end of two in tandem aligned D–J–C gene clusters, each composed of a single TRBD, 6 TRBJ and one TRBC genes, followed by a single TRBV gene with an inverted transcriptional orientation. The TRB genes are distributed in less than 300 kb, making the canine locus, one of the smaller mammalian TRB locus studied so far. The small size may be ascribed to reduced gene duplication occurrences and a lower density of total interspersed repeats compared to humans and mice. Despite the low TRBV gene content, a large and diversified beta chain repertoire is displayed in the dog peripheral blood. A full usage of TRBV and TRBJ genes, including pseudogenes, and a high level of allelic polymorphism contribute to generate diversity. Finally, this study suggests that the overall TRB locus organization is evolutionarily conserved supporting the dog as a highly suited model system for immune development and diseases. |
author2 |
Mineccia, M Massari, S. Linguiti, G. Ceci, Luigi Ciccarese, Salvatrice Maria Antonacci, Rachele |
format |
Article in Journal/Newspaper |
author |
Mineccia, M Massari, S. Linguiti, G. CECI, Luigi CICCARESE, Salvatrice Maria ANTONACCI, Rachele |
spellingShingle |
Mineccia, M Massari, S. Linguiti, G. CECI, Luigi CICCARESE, Salvatrice Maria ANTONACCI, Rachele New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis |
author_facet |
Mineccia, M Massari, S. Linguiti, G. CECI, Luigi CICCARESE, Salvatrice Maria ANTONACCI, Rachele |
author_sort |
Mineccia, M |
title |
New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis |
title_short |
New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis |
title_full |
New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis |
title_fullStr |
New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis |
title_full_unstemmed |
New insight into the genomic structure of dog T cell receptor beta (TRB) locus inferred from expression analysis |
title_sort |
new insight into the genomic structure of dog t cell receptor beta (trb) locus inferred from expression analysis |
publishDate |
2012 |
url |
http://hdl.handle.net/11586/126658 https://doi.org/10.1016/j.dci.2012.03.010 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
info:eu-repo/semantics/altIdentifier/pmid/22465586 info:eu-repo/semantics/altIdentifier/wos/WOS:000306891400007 volume:37 issue:2 firstpage:279 lastpage:293 numberofpages:15 journal:DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY http://hdl.handle.net/11586/126658 doi:10.1016/j.dci.2012.03.010 info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-84862146557 |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1016/j.dci.2012.03.010 |
container_title |
Developmental & Comparative Immunology |
container_volume |
37 |
container_issue |
2 |
container_start_page |
279 |
op_container_end_page |
293 |
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1784268369018486784 |