Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar
International audience The leaves of Dilobeia thouarsii (Roemer and Schulte), a tree that is endemic to Madagascar (Proteaceae), are used in traditional Malagasy medicine to treat bacterial skin infections and wounds. This study investigated the in vitro antibacterial activities of D. thouarsii leaf...
Published in: | South African Journal of Botany |
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ftunivavignon:oai:HAL:hal-01188116v1 2024-02-11T09:58:28+01:00 Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar Razafintsalama, Vahinalahaja Sarter, Samira Mambu, Lengo, Angèle Randrianarivo, Ranjana Petit, Thomas Rajaonarison, Jean F. Mertz, Christian Rakoto, Danielle Jeannoda, Victor Molécules de Communication et Adaptation des Micro-Organismes (MCAM) Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) Université d'Antananarivo Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA) Université de La Réunion (UR) Département de Biochimie Fondamentale et Appliquée 2013 https://hal.science/hal-01188116 https://hal.science/hal-01188116/document https://hal.science/hal-01188116/file/1-s2.0-S025462991300207X-main.pdf https://doi.org/10.1016/j.sajb.2013.02.171 en eng HAL CCSD Elsevier info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sajb.2013.02.171 hal-01188116 https://hal.science/hal-01188116 https://hal.science/hal-01188116/document https://hal.science/hal-01188116/file/1-s2.0-S025462991300207X-main.pdf doi:10.1016/j.sajb.2013.02.171 http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 0254-6299 South African Journal of Botany https://hal.science/hal-01188116 South African Journal of Botany, 2013, 87, pp.1--3. ⟨10.1016/j.sajb.2013.02.171⟩ [CHIM]Chemical Sciences info:eu-repo/semantics/article Journal articles 2013 ftunivavignon https://doi.org/10.1016/j.sajb.2013.02.171 2024-01-23T23:41:12Z International audience The leaves of Dilobeia thouarsii (Roemer and Schulte), a tree that is endemic to Madagascar (Proteaceae), are used in traditional Malagasy medicine to treat bacterial skin infections and wounds. This study investigated the in vitro antibacterial activities of D. thouarsii leaf extracts and identified the bioactive compounds with the aim of providing a scientific basis for its use against skin diseases. Using broth microdilution method for leaf crude extract and its compounds, we investigated inhibition of the growth of Bacillus cereus, Bacillus megaterium, Staphylococcus aureus, Enterococcus faecalis, Vibrio harveyi, Vibrio fisheri, Salmonella Typhimurium, Salmonella antarctica, Escherichia coli, and Klebsiella pneumoniae. The two purified phenolic compounds from leaf ethyl acetate extracts (1, 2) were found to be more active than the crude extract itself. The structure of the two compounds was elucidated by NMR and mass spectrometry: compound 1 was identified as 4-aminophenol and compound 2 as 4-hydroxybenzaldehyde. A marked inhibitory effect (MIC < 0.1 mg/ml) was found against S. aureus, which is a major agent in skin infections. We observed moderate activities (MIC values of between 0.1 and 0.5 mg/ml) for E. faecalis, Vibrio spp., and Bacillus spp. Neither compound was active against Salmonella spp., E. coli and K. pneumoniae (MICs > 1 mg/ml). To conclude, the high antimicrobial activity of D. thouarsii leaf extracts against S. aureus supports its traditional use to treat skin infections. Article in Journal/Newspaper Antarc* Antarctica Université d'Avignon et des Pays de Vaucluse: HAL South African Journal of Botany 87 1 3 |
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Université d'Avignon et des Pays de Vaucluse: HAL |
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[CHIM]Chemical Sciences |
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[CHIM]Chemical Sciences Razafintsalama, Vahinalahaja Sarter, Samira Mambu, Lengo, Angèle Randrianarivo, Ranjana Petit, Thomas Rajaonarison, Jean F. Mertz, Christian Rakoto, Danielle Jeannoda, Victor Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar |
topic_facet |
[CHIM]Chemical Sciences |
description |
International audience The leaves of Dilobeia thouarsii (Roemer and Schulte), a tree that is endemic to Madagascar (Proteaceae), are used in traditional Malagasy medicine to treat bacterial skin infections and wounds. This study investigated the in vitro antibacterial activities of D. thouarsii leaf extracts and identified the bioactive compounds with the aim of providing a scientific basis for its use against skin diseases. Using broth microdilution method for leaf crude extract and its compounds, we investigated inhibition of the growth of Bacillus cereus, Bacillus megaterium, Staphylococcus aureus, Enterococcus faecalis, Vibrio harveyi, Vibrio fisheri, Salmonella Typhimurium, Salmonella antarctica, Escherichia coli, and Klebsiella pneumoniae. The two purified phenolic compounds from leaf ethyl acetate extracts (1, 2) were found to be more active than the crude extract itself. The structure of the two compounds was elucidated by NMR and mass spectrometry: compound 1 was identified as 4-aminophenol and compound 2 as 4-hydroxybenzaldehyde. A marked inhibitory effect (MIC < 0.1 mg/ml) was found against S. aureus, which is a major agent in skin infections. We observed moderate activities (MIC values of between 0.1 and 0.5 mg/ml) for E. faecalis, Vibrio spp., and Bacillus spp. Neither compound was active against Salmonella spp., E. coli and K. pneumoniae (MICs > 1 mg/ml). To conclude, the high antimicrobial activity of D. thouarsii leaf extracts against S. aureus supports its traditional use to treat skin infections. |
author2 |
Molécules de Communication et Adaptation des Micro-Organismes (MCAM) Muséum national d'Histoire naturelle (MNHN)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS) Université d'Antananarivo Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments (LCSNSA) Université de La Réunion (UR) Département de Biochimie Fondamentale et Appliquée |
format |
Article in Journal/Newspaper |
author |
Razafintsalama, Vahinalahaja Sarter, Samira Mambu, Lengo, Angèle Randrianarivo, Ranjana Petit, Thomas Rajaonarison, Jean F. Mertz, Christian Rakoto, Danielle Jeannoda, Victor |
author_facet |
Razafintsalama, Vahinalahaja Sarter, Samira Mambu, Lengo, Angèle Randrianarivo, Ranjana Petit, Thomas Rajaonarison, Jean F. Mertz, Christian Rakoto, Danielle Jeannoda, Victor |
author_sort |
Razafintsalama, Vahinalahaja |
title |
Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar |
title_short |
Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar |
title_full |
Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar |
title_fullStr |
Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar |
title_full_unstemmed |
Antimicrobial activities of Dilobeia thouarsii Roemer and Schulte, a traditional medicinal plant from Madagascar |
title_sort |
antimicrobial activities of dilobeia thouarsii roemer and schulte, a traditional medicinal plant from madagascar |
publisher |
HAL CCSD |
publishDate |
2013 |
url |
https://hal.science/hal-01188116 https://hal.science/hal-01188116/document https://hal.science/hal-01188116/file/1-s2.0-S025462991300207X-main.pdf https://doi.org/10.1016/j.sajb.2013.02.171 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
ISSN: 0254-6299 South African Journal of Botany https://hal.science/hal-01188116 South African Journal of Botany, 2013, 87, pp.1--3. ⟨10.1016/j.sajb.2013.02.171⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.sajb.2013.02.171 hal-01188116 https://hal.science/hal-01188116 https://hal.science/hal-01188116/document https://hal.science/hal-01188116/file/1-s2.0-S025462991300207X-main.pdf doi:10.1016/j.sajb.2013.02.171 |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1016/j.sajb.2013.02.171 |
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South African Journal of Botany |
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87 |
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1790594128530112512 |