Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy
Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIPHLL) and Candida antarctica lipase A (LIPCA) within a transparent latex coating. Addit...
Published in: | Colloids and Surfaces B: Biointerfaces |
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Language: | English |
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Online Access: | http://hdl.handle.net/2292/8558 https://doi.org/10.1016/j.colsurfb.2010.08.042 |
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ftunivauckland:oai:researchspace.auckland.ac.nz:2292/8558 2023-05-15T13:59:29+02:00 Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy Jayawardena, MB Yee, LH Rainbow, IJ Bergquist, P Such, C Steinberg, PD Kjelleberg, SJ 2011 http://hdl.handle.net/2292/8558 https://doi.org/10.1016/j.colsurfb.2010.08.042 EN eng ELSEVIER SCIENCE BV COLLOID SURFACE B Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0927-7765/ https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm Copyright: ELSEVIER SCIENCE BV http://purl.org/eprint/accessRights/RestrictedAccess http://dx.doi.org/10.1016/j.colsurfb.2010.08.042 Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA Journal Article 2011 ftunivauckland https://doi.org/10.1016/j.colsurfb.2010.08.042 2013-01-22T00:21:27Z Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIPHLL) and Candida antarctica lipase A (LIPCA) within a transparent latex coating. Addition of Teric Surfactants (C16 non-ionic Teric 475, 1.8% (w/w) or C10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIPHLL and LIPCA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. Article in Journal/Newspaper Antarc* Antarctica University of Auckland Research Repository - ResearchSpace Colloids and Surfaces B: Biointerfaces 82 2 291 296 |
institution |
Open Polar |
collection |
University of Auckland Research Repository - ResearchSpace |
op_collection_id |
ftunivauckland |
language |
English |
topic |
Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA |
spellingShingle |
Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA Jayawardena, MB Yee, LH Rainbow, IJ Bergquist, P Such, C Steinberg, PD Kjelleberg, SJ Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
topic_facet |
Confocal laser scanning microscopy Lipase Catalytic coating Surface enzyme activity Surfactant Latex COATINGS EPICOCCONONE IMMOBILIZATION MECHANISM SILICA |
description |
Confocal laser scanning microscopy (CLSM) in combination with a fluorescently labeling enzyme dye, LavaPurple™, was demonstrated as a technique for the visualization of Thermomyces (Humicola) lanuginosa lipase (LIPHLL) and Candida antarctica lipase A (LIPCA) within a transparent latex coating. Addition of Teric Surfactants (C16 non-ionic Teric 475, 1.8% (w/w) or C10 non-ionic Teric 460, 2.0% (w/w)) significantly increased the accumulation of both LIPHLL and LIPCA to the surface of a latex coating. An α-naphthyl acetate substrate assay was used to quantify the accumulated lipase. The results derived from the acetate assay correlated with the enzyme accumulation (at the surface) observed in the CLSM images of the latex coating. This correlation demonstrated that the increased enzyme accumulation within the top 2 μm of the latex film was responsible for the increase in surface enzymatic activity. The combination of CLSM imagery and quantifiable image analysis provided a valuable tool for the optimization of surfactant concentrations for maximizing the activity of an enzyme (and potentially other additives) within a latex coating. |
format |
Article in Journal/Newspaper |
author |
Jayawardena, MB Yee, LH Rainbow, IJ Bergquist, P Such, C Steinberg, PD Kjelleberg, SJ |
author_facet |
Jayawardena, MB Yee, LH Rainbow, IJ Bergquist, P Such, C Steinberg, PD Kjelleberg, SJ |
author_sort |
Jayawardena, MB |
title |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_short |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_full |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_fullStr |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_full_unstemmed |
Surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
title_sort |
surfactant enhanced lipase containing films characterized by confocal laser scanning microscopy |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2011 |
url |
http://hdl.handle.net/2292/8558 https://doi.org/10.1016/j.colsurfb.2010.08.042 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
http://dx.doi.org/10.1016/j.colsurfb.2010.08.042 |
op_relation |
COLLOID SURFACE B |
op_rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0927-7765/ https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm Copyright: ELSEVIER SCIENCE BV http://purl.org/eprint/accessRights/RestrictedAccess |
op_doi |
https://doi.org/10.1016/j.colsurfb.2010.08.042 |
container_title |
Colloids and Surfaces B: Biointerfaces |
container_volume |
82 |
container_issue |
2 |
container_start_page |
291 |
op_container_end_page |
296 |
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1766268059321892864 |