Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations
The effect of altered oxygen transport potential on behavioural responses to environmental hypoxia was tested experimentally in snapper, Pagrus auratus, treated with a haemolytic agent (phenylhydrazine) or a sham protocol. Standard metabolic rate was not different between anaemic and normocythaemic...
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The Company of Biologists Ltd
2011
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Online Access: | http://hdl.handle.net/2292/16288 https://doi.org/10.1242/jeb.057091 |
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ftunivauckland:oai:researchspace.auckland.ac.nz:2292/16288 2023-05-15T15:27:46+02:00 Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations Cook, DG Wells, RMG Herbert, NA 2011 http://hdl.handle.net/2292/16288 https://doi.org/10.1242/jeb.057091 EN eng The Company of Biologists Ltd Journal of Experimental Biology Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0022-0949/ https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm Copyright: The Company of Biologists Ltd http://purl.org/eprint/accessRights/RestrictedAccess http://dx.doi.org/10.1242/jeb.057091 avoidance hypoxia anaemia metabolic scope haemoglobin P-crit COD GADUS-MORHUA EUROPEAN SEA BASS ATLANTIC COD PROGRESSIVE HYPOXIA DISSOLVED-OXYGEN SWIMMING SPEED ONCORHYNCHUS-MYKISS RAINBOW-TROUT RESPONSES TOLERANCE Journal Article 2011 ftunivauckland https://doi.org/10.1242/jeb.057091 2013-12-07T09:24:15Z The effect of altered oxygen transport potential on behavioural responses to environmental hypoxia was tested experimentally in snapper, Pagrus auratus, treated with a haemolytic agent (phenylhydrazine) or a sham protocol. Standard metabolic rate was not different between anaemic and normocythaemic snapper (Hct=6.7 and 25.7 g dl^{-1}, respectively), whereas maximum metabolic rate, and hence aerobic scope (AS), was consistently reduced in anaemic groups at all levels of water PO_{2} investigated (P<0.01). This reduction of AS conferred a higher critical oxygen limit (P_{crit}) to anaemic fish (8.6±0.6 kPa) compared with normocythaemic fish (5.3±0.4 kPa), thus demonstrating reduced hypoxic tolerance in anaemic groups. In behavioural choice experiments, the critical avoidance PO_{2} in anaemic fish was 6.6±2.5 kPa compared with 2.9±0.5 kPa for controls (P<0.01). Behavioural avoidance was not associated with modulation of swimming speed. Despite differences in physiological and behavioural parameters, both groups avoided low PO_{2} just below their P_{crit}, indicating that avoidance was triggered consistently when AS limits were reached and anaerobic metabolism was unavoidable. This was confirmed by high levels of plasma lactate in both treatments at the point of avoidance. This is the first experimental demonstration of avoidance behaviour being modulated by internal physiological state. From an ecological perspective, fish with disturbed oxygen delivery potential arising from anaemia, pollution or stress are likely to avoid environmental hypoxia at a higher PO_{2} than normal fish. Article in Journal/Newspaper atlantic cod Gadus morhua University of Auckland Research Repository - ResearchSpace Journal of Experimental Biology 214 17 2927 2934 |
institution |
Open Polar |
collection |
University of Auckland Research Repository - ResearchSpace |
op_collection_id |
ftunivauckland |
language |
English |
topic |
avoidance hypoxia anaemia metabolic scope haemoglobin P-crit COD GADUS-MORHUA EUROPEAN SEA BASS ATLANTIC COD PROGRESSIVE HYPOXIA DISSOLVED-OXYGEN SWIMMING SPEED ONCORHYNCHUS-MYKISS RAINBOW-TROUT RESPONSES TOLERANCE |
spellingShingle |
avoidance hypoxia anaemia metabolic scope haemoglobin P-crit COD GADUS-MORHUA EUROPEAN SEA BASS ATLANTIC COD PROGRESSIVE HYPOXIA DISSOLVED-OXYGEN SWIMMING SPEED ONCORHYNCHUS-MYKISS RAINBOW-TROUT RESPONSES TOLERANCE Cook, DG Wells, RMG Herbert, NA Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations |
topic_facet |
avoidance hypoxia anaemia metabolic scope haemoglobin P-crit COD GADUS-MORHUA EUROPEAN SEA BASS ATLANTIC COD PROGRESSIVE HYPOXIA DISSOLVED-OXYGEN SWIMMING SPEED ONCORHYNCHUS-MYKISS RAINBOW-TROUT RESPONSES TOLERANCE |
description |
The effect of altered oxygen transport potential on behavioural responses to environmental hypoxia was tested experimentally in snapper, Pagrus auratus, treated with a haemolytic agent (phenylhydrazine) or a sham protocol. Standard metabolic rate was not different between anaemic and normocythaemic snapper (Hct=6.7 and 25.7 g dl^{-1}, respectively), whereas maximum metabolic rate, and hence aerobic scope (AS), was consistently reduced in anaemic groups at all levels of water PO_{2} investigated (P<0.01). This reduction of AS conferred a higher critical oxygen limit (P_{crit}) to anaemic fish (8.6±0.6 kPa) compared with normocythaemic fish (5.3±0.4 kPa), thus demonstrating reduced hypoxic tolerance in anaemic groups. In behavioural choice experiments, the critical avoidance PO_{2} in anaemic fish was 6.6±2.5 kPa compared with 2.9±0.5 kPa for controls (P<0.01). Behavioural avoidance was not associated with modulation of swimming speed. Despite differences in physiological and behavioural parameters, both groups avoided low PO_{2} just below their P_{crit}, indicating that avoidance was triggered consistently when AS limits were reached and anaerobic metabolism was unavoidable. This was confirmed by high levels of plasma lactate in both treatments at the point of avoidance. This is the first experimental demonstration of avoidance behaviour being modulated by internal physiological state. From an ecological perspective, fish with disturbed oxygen delivery potential arising from anaemia, pollution or stress are likely to avoid environmental hypoxia at a higher PO_{2} than normal fish. |
format |
Article in Journal/Newspaper |
author |
Cook, DG Wells, RMG Herbert, NA |
author_facet |
Cook, DG Wells, RMG Herbert, NA |
author_sort |
Cook, DG |
title |
Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations |
title_short |
Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations |
title_full |
Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations |
title_fullStr |
Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations |
title_full_unstemmed |
Anaemia adjusts the aerobic physiology of snapper (Pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations |
title_sort |
anaemia adjusts the aerobic physiology of snapper (pagrus auratus) and modulates hypoxia avoidance behaviour during oxygen choice presentations |
publisher |
The Company of Biologists Ltd |
publishDate |
2011 |
url |
http://hdl.handle.net/2292/16288 https://doi.org/10.1242/jeb.057091 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
http://dx.doi.org/10.1242/jeb.057091 |
op_relation |
Journal of Experimental Biology |
op_rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0022-0949/ https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm Copyright: The Company of Biologists Ltd http://purl.org/eprint/accessRights/RestrictedAccess |
op_doi |
https://doi.org/10.1242/jeb.057091 |
container_title |
Journal of Experimental Biology |
container_volume |
214 |
container_issue |
17 |
container_start_page |
2927 |
op_container_end_page |
2934 |
_version_ |
1766358175238324224 |