Host and Water Microbiota Are Differentially Linked to Potential Human Pathogen Accumulation in Oysters

• Published in: Applied and Environmental Microbiology
• Main Authors: Diner, R.E., Zimmer-Faust, A., Cooksey, E., Allard, S., Kodera, S.M., Kunselman, E., Garodia, Y., Verhougstraete, M.P., Allen, A.E., Griffith, J., Gilbert, J.A.
• Other Authors: Environment Exposure Science and Risk Assessment Center, University of Arizona, Mel Enid Zuckerman College of Public Health, University of Arizona
• Format: Article in Journal/Newspaper
• Language: English
• Published: American Society for Microbiology 2023
• Subjects: aquaculture; environmental microbiology; fecal organisms; oyster microbiome; Pacific oyster; shellfish; Vibrio parahaemolyticus; Vibrio vulnificus
• Online Access: http://hdl.handle.net/10150/674556; https://doi.org/10.1128/aem.00318-23

Oysters play an important role in coastal ecology and are a globally popular seafood source. However, their filter-feeding lifestyle enables coastal pathogens, toxins, and pollutants to accumulate in their tissues, potentially endangering human health. While pathogen concentrations in coastal waters are often linked to environmental conditions and runoff events, these do not always correlate with pathogen concentrations in oysters. Additional factors related to the microbial ecology of pathogenic bacteria and their relationship with oyster hosts likely play a role in accumulation but are poorly understood. In this study, we investigated whether microbial communities in water and oysters were linked to accumulation of Vibrio parahaemolyticus, Vibrio vulnificus, or fecal indicator bacteria. Site-specific environmental conditions significantly influenced microbial communities and potential pathogen concentrations in water. Oyster microbial communities, however, exhibited less variability in microbial community diversity and accumulation of target bacteria overall and were less impacted by environmental differences between sites. Instead, changes in specific microbial taxa in oyster and water samples, particularly in oyster digestive glands, were linked to elevated levels of potential pathogens. For example, increased levels of V. parahaemolyticus were associated with higher relative abundances of cyanobacteria, which could represent an environmental vector for Vibrio spp. transport, and with decreased relative abundance of Mycoplasma and other key members of the oyster digestive gland microbiota. These findings suggest that host and microbial factors, in addition to environmental variables, may influence pathogen accumulation in oysters. © 2023 Diner et al. Open access article This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at [e-mail].