A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations
Abstract: Three sister species of rough periwinkles, viz. Littorina saxatilis (Olivi 1792), L. arcana (Hannaford Ellis 1978) and L. compressa (Jeffreys 1865) from the Barents Sea (Russia), the White Sea (Russia) and the Norwegian Sea (Norway) were studied. The identification of two sibling species L...
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ftunivantwerpen:c:irua:78911 2023-07-16T03:57:42+02:00 A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations Mikhailova, Natalia A. Gracheva, Yulia A. Backeljau, Thierry Granovitch, Andrey I. 2009 pdf https://hdl.handle.net/10067/789110151162165141 eng eng info:eu-repo/semantics/altIdentifier/isi/000271307300010 info:eu-repo/semantics/altIdentifier/doi/10.1007/S10709-009-9397-4 info:eu-repo/semantics/closedAccess 0016-6707 Genetica Biology info:eu-repo/semantics/article 2009 ftunivantwerpen https://doi.org/10.1007/S10709-009-9397-4 2023-06-26T22:14:06Z Abstract: Three sister species of rough periwinkles, viz. Littorina saxatilis (Olivi 1792), L. arcana (Hannaford Ellis 1978) and L. compressa (Jeffreys 1865) from the Barents Sea (Russia), the White Sea (Russia) and the Norwegian Sea (Norway) were studied. The identification of two sibling species L. saxatilis and L. arcana is often difficult as both species have extremely similar shell morphology and reproductive systems. Only mature females can be unambiguously distinguished, with a jelly gland present in female L. arcana, but which is replaced by a brood pouch containing developing embryos in L. saxatilis. No clear-cut diagnostic features have been found to discriminate between males or juveniles of the two species. The very first diagnostic DNA marker (DNA fragment A2.8, 271 bp length) for L. arcana and L. saxatilis separation was developed. The marker was derived from apparently species-specific L. arcana DNA fragments obtained via Random Amplified Polymorphic DNA (RAPD) analysis. This fragment was cloned and sequenced, whereupon specific primers were designed and the amplification was surveyed in a large number of morphologically well-identified females of both species. Subsequently, the specific DNA marker was used for the identification of male L. arcana and partners in copulating pairs. In this way, we obtained evidence of possible interspecific hybridization between the sibling species L. arcana and L. saxatilis living in sympatry in natural populations: the presence of A2.8 fragment in 12% of morphologically well identified L. saxatilis females and its absence in 14% of morphologically well identified L. arcana females. The A2.8 fragment never amplified in L. saxatilis from sites without L. arcana. The A2.8 fragment did not amplify in L. compressa, not even in microsympatric populations, and we did not observe interspecific copulations between L. arcana and L. compressa. Article in Journal/Newspaper Barents Sea Norwegian Sea White Sea IRUA - Institutional Repository van de Universiteit Antwerpen Barents Sea Norway Norwegian Sea White Sea Genetica 137 3 333 340 |
institution |
Open Polar |
collection |
IRUA - Institutional Repository van de Universiteit Antwerpen |
op_collection_id |
ftunivantwerpen |
language |
English |
topic |
Biology |
spellingShingle |
Biology Mikhailova, Natalia A. Gracheva, Yulia A. Backeljau, Thierry Granovitch, Andrey I. A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations |
topic_facet |
Biology |
description |
Abstract: Three sister species of rough periwinkles, viz. Littorina saxatilis (Olivi 1792), L. arcana (Hannaford Ellis 1978) and L. compressa (Jeffreys 1865) from the Barents Sea (Russia), the White Sea (Russia) and the Norwegian Sea (Norway) were studied. The identification of two sibling species L. saxatilis and L. arcana is often difficult as both species have extremely similar shell morphology and reproductive systems. Only mature females can be unambiguously distinguished, with a jelly gland present in female L. arcana, but which is replaced by a brood pouch containing developing embryos in L. saxatilis. No clear-cut diagnostic features have been found to discriminate between males or juveniles of the two species. The very first diagnostic DNA marker (DNA fragment A2.8, 271 bp length) for L. arcana and L. saxatilis separation was developed. The marker was derived from apparently species-specific L. arcana DNA fragments obtained via Random Amplified Polymorphic DNA (RAPD) analysis. This fragment was cloned and sequenced, whereupon specific primers were designed and the amplification was surveyed in a large number of morphologically well-identified females of both species. Subsequently, the specific DNA marker was used for the identification of male L. arcana and partners in copulating pairs. In this way, we obtained evidence of possible interspecific hybridization between the sibling species L. arcana and L. saxatilis living in sympatry in natural populations: the presence of A2.8 fragment in 12% of morphologically well identified L. saxatilis females and its absence in 14% of morphologically well identified L. arcana females. The A2.8 fragment never amplified in L. saxatilis from sites without L. arcana. The A2.8 fragment did not amplify in L. compressa, not even in microsympatric populations, and we did not observe interspecific copulations between L. arcana and L. compressa. |
format |
Article in Journal/Newspaper |
author |
Mikhailova, Natalia A. Gracheva, Yulia A. Backeljau, Thierry Granovitch, Andrey I. |
author_facet |
Mikhailova, Natalia A. Gracheva, Yulia A. Backeljau, Thierry Granovitch, Andrey I. |
author_sort |
Mikhailova, Natalia A. |
title |
A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations |
title_short |
A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations |
title_full |
A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations |
title_fullStr |
A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations |
title_full_unstemmed |
A potential species-specific molecular marker suggests interspecific hybridization between sibling species **Littorina arcana** and **L. saxatilis** (Mollusca, Caenogastropoda) in natural populations |
title_sort |
potential species-specific molecular marker suggests interspecific hybridization between sibling species **littorina arcana** and **l. saxatilis** (mollusca, caenogastropoda) in natural populations |
publishDate |
2009 |
url |
https://hdl.handle.net/10067/789110151162165141 |
geographic |
Barents Sea Norway Norwegian Sea White Sea |
geographic_facet |
Barents Sea Norway Norwegian Sea White Sea |
genre |
Barents Sea Norwegian Sea White Sea |
genre_facet |
Barents Sea Norwegian Sea White Sea |
op_source |
0016-6707 Genetica |
op_relation |
info:eu-repo/semantics/altIdentifier/isi/000271307300010 info:eu-repo/semantics/altIdentifier/doi/10.1007/S10709-009-9397-4 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1007/S10709-009-9397-4 |
container_title |
Genetica |
container_volume |
137 |
container_issue |
3 |
container_start_page |
333 |
op_container_end_page |
340 |
_version_ |
1771544367379513344 |