Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains
Abstract: Although polar regions are characterised by extreme environment conditions with overall low temperatures and often pronounced diurnal to seasonal temperature fluctuations, diatoms (Bacillariophyceae) thrive in a wide range of nonmarine polar habitats, suggesting that they are well adapted...
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ftunivantwerpen:c:irua:161416 2023-07-16T03:53:07+02:00 Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains Hejdukova, Eva Pinseel, Eveline Vanormelingen, Pieter Nedbalova, Linda Elster, Josef Vyverman, Wim Sabbe, Koen 2019 https://hdl.handle.net/10067/1614160151162165141 eng eng info:eu-repo/semantics/altIdentifier/doi/10.1080/00318884.2019.1591835 info:eu-repo/semantics/altIdentifier/isi/000475669100005 info:eu-repo/semantics/closedAccess 0031-8884 Phycologia Biology info:eu-repo/semantics/article 2019 ftunivantwerpen https://doi.org/10.1080/00318884.2019.1591835 2023-06-26T22:29:01Z Abstract: Although polar regions are characterised by extreme environment conditions with overall low temperatures and often pronounced diurnal to seasonal temperature fluctuations, diatoms (Bacillariophyceae) thrive in a wide range of nonmarine polar habitats, suggesting that they are well adapted to these harsh conditions. In this study, tolerance to freezing of vegetative and resting cells of 17 polar and nine temperate diatom strains, belonging to different morphospecies and originating from both freshwater and terrestrial habitats, was experimentally tested. Most strains were newly isolated from sites in Maritime Antarctica (James Ross Island and Vega Island), the High Arctic (Spitsbergen) and Europe. Cultures were exposed to five freezing treatments differing in temperature (-4, -20, -40 and -180 degrees C), freezing rate (gradual, abrupt), time (1 h, 12 h) and thawing rate (slow, fast). Results indicated that diatoms were sensitive to experimental freezing. Freezing temperatures had a significant effect on strain survival: all strains survived -4 degrees C; most survived -20 degrees C; five survived -40 and four of these (all belonging to the Pinnularia borealis complex) survived freezing in liquid nitrogen (-180 degrees C). The cooling and thawing rate had a significant impact on survival: abrupt cooling and slow thawing resulted in much lower survival rates than gradual cooling and fast thawing. Resting cells showed better growth than vegetative cells after freezing treatment but only in the -4 degrees C treatment. Surprisingly, no striking differences in growth recovery were observed between polar and temperate strains. Article in Journal/Newspaper Antarc* Antarctica Arctic James Ross Island Ross Island Vega Island Spitsbergen IRUA - Institutional Repository van de Universiteit Antwerpen Arctic Ross Island Vega Island ENVELOPE(-57.500,-57.500,-63.833,-63.833) Phycologia 58 4 382 392 |
institution |
Open Polar |
collection |
IRUA - Institutional Repository van de Universiteit Antwerpen |
op_collection_id |
ftunivantwerpen |
language |
English |
topic |
Biology |
spellingShingle |
Biology Hejdukova, Eva Pinseel, Eveline Vanormelingen, Pieter Nedbalova, Linda Elster, Josef Vyverman, Wim Sabbe, Koen Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains |
topic_facet |
Biology |
description |
Abstract: Although polar regions are characterised by extreme environment conditions with overall low temperatures and often pronounced diurnal to seasonal temperature fluctuations, diatoms (Bacillariophyceae) thrive in a wide range of nonmarine polar habitats, suggesting that they are well adapted to these harsh conditions. In this study, tolerance to freezing of vegetative and resting cells of 17 polar and nine temperate diatom strains, belonging to different morphospecies and originating from both freshwater and terrestrial habitats, was experimentally tested. Most strains were newly isolated from sites in Maritime Antarctica (James Ross Island and Vega Island), the High Arctic (Spitsbergen) and Europe. Cultures were exposed to five freezing treatments differing in temperature (-4, -20, -40 and -180 degrees C), freezing rate (gradual, abrupt), time (1 h, 12 h) and thawing rate (slow, fast). Results indicated that diatoms were sensitive to experimental freezing. Freezing temperatures had a significant effect on strain survival: all strains survived -4 degrees C; most survived -20 degrees C; five survived -40 and four of these (all belonging to the Pinnularia borealis complex) survived freezing in liquid nitrogen (-180 degrees C). The cooling and thawing rate had a significant impact on survival: abrupt cooling and slow thawing resulted in much lower survival rates than gradual cooling and fast thawing. Resting cells showed better growth than vegetative cells after freezing treatment but only in the -4 degrees C treatment. Surprisingly, no striking differences in growth recovery were observed between polar and temperate strains. |
format |
Article in Journal/Newspaper |
author |
Hejdukova, Eva Pinseel, Eveline Vanormelingen, Pieter Nedbalova, Linda Elster, Josef Vyverman, Wim Sabbe, Koen |
author_facet |
Hejdukova, Eva Pinseel, Eveline Vanormelingen, Pieter Nedbalova, Linda Elster, Josef Vyverman, Wim Sabbe, Koen |
author_sort |
Hejdukova, Eva |
title |
Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains |
title_short |
Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains |
title_full |
Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains |
title_fullStr |
Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains |
title_full_unstemmed |
Tolerance of pennate diatoms (Bacillariophyceae) to experimental freezing: comparison of polar and temperate strains |
title_sort |
tolerance of pennate diatoms (bacillariophyceae) to experimental freezing: comparison of polar and temperate strains |
publishDate |
2019 |
url |
https://hdl.handle.net/10067/1614160151162165141 |
long_lat |
ENVELOPE(-57.500,-57.500,-63.833,-63.833) |
geographic |
Arctic Ross Island Vega Island |
geographic_facet |
Arctic Ross Island Vega Island |
genre |
Antarc* Antarctica Arctic James Ross Island Ross Island Vega Island Spitsbergen |
genre_facet |
Antarc* Antarctica Arctic James Ross Island Ross Island Vega Island Spitsbergen |
op_source |
0031-8884 Phycologia |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1080/00318884.2019.1591835 info:eu-repo/semantics/altIdentifier/isi/000475669100005 |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1080/00318884.2019.1591835 |
container_title |
Phycologia |
container_volume |
58 |
container_issue |
4 |
container_start_page |
382 |
op_container_end_page |
392 |
_version_ |
1771548686785970176 |